Phospho-EIF6 (Ser235) Antibody - #AF8474
| 製品: | Phospho-EIF6 (Ser235) Antibody |
| カタログ: | AF8474 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-EIF6 (Ser235) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 27kDa; 27kD(Calculated). |
| ユニプロット: | P56537 |
| RRID: | AB_2840528 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8474, RRID:AB_2840528.
折りたたみ／展開
b(2)gcn; B(2)GCN homolog; B4 integrin interactor; Binding protein of beta-4 integrin; CAB; eIF-6; EIF3A; EIF6; Eukaryotic translation initiation factor 3A; Eukaryotic translation initiation factor 6; IF6_HUMAN; ITGB4BP; OK/SW-cl.27; p27 beta 4 integrin binding protein; p27(BBP); p27BBP; RP4-614O4.1;
免疫原
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
- P56537 IF6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P56537 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| C11 | S-Nitrosylation | Uniprot | |
| C15 | S-Nitrosylation | Uniprot | |
| R85 | Methylation | Uniprot | |
| R95 | Methylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S175 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| T185 | Phosphorylation | Uniprot | |
| K223 | Acetylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T231 | Phosphorylation | Uniprot | |
| T234 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | P17252 (PRKCA) , P05771 (PRKCB) | Uniprot |
| S239 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| T245 | Phosphorylation | Uniprot |
研究背景
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).
Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.
Cytoplasm. Nucleus>Nucleolus.
Note: Shuttles between cytoplasm and nucleus/nucleolus.
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
Monomer. Associates with the 60S ribosomal subunit. Interacts with RACK1. Interacts with DICER1, AGO2, TARBP2, MOV10 and RPL7A; they form a large RNA-induced silencing complex (RISC).
Belongs to the eIF-6 family.
研究領域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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