Phospho-NDEL1 (Thr219) Antibody - #AF8492
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8492, RRID:AB_2840546.
折りたたみ／展開
A. nidulans; DKFZp451M0318; ENDOOLIGOPEPTIDASE A; EOPA; MITAP 1; MITAP1; Mitosin associated protein 1; Mitosin associated protein MITAP1; Mitosin-associated protein 1; Ndel 1; NDEL1; NDEL1_HUMAN; Nuclear distribution gene E like homolog 1; Nuclear distribution protein nudE like 1; Nuclear distribution protein nudE-like 1; NUDE like protein; NudE nuclear distribution gene E homolog like 1 A. nidulans; NudE nuclear distribution gene E homolog like 1; NUDEL; Protein Nudel;
免疫原
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
- Q9GZM8 NDEL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFEQRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAVQASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGAVNGFDPAPPPPGLGSSRPSSAPGMLPLSV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9GZM8 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S44 | Phosphorylation | Uniprot | |
| Y87 | Phosphorylation | Uniprot | |
| T132 | Phosphorylation | Uniprot | |
| S135 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | P06493 (CDK1) , P28482 (MAPK1) | Uniprot |
| S231 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T245 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) | Uniprot |
| S251 | Phosphorylation | O14965 (AURKA) | Uniprot |
| K269 | Ubiquitination | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot |
研究背景
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity).
Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE.
Palmitoylation at Cys-273 reduces affinity for dynein.
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle.
Note: Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner.
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Interacts with PLEKHM1 (via N- and C-terminus) (By similarity). Interacts with YWHAE. Interacts directly with NEFL and indirectly with NEFH. Interacts with microtubules (By similarity). Self-associates. Interacts with DISC1, dynein, dynactin, tubulin gamma, KATNA1, KATNB1, PAFAH1B1, PCM1 and PCNT. Interacts (via C-terminus) with CENPF. Interacts with ZNF365.
Belongs to the nudE family.
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