Phospho-PNKP (Ser114) Antibody - #AF8503
| 製品: | Phospho-PNKP (Ser114) Antibody |
| カタログ: | AF8503 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-PNKP (Ser114) |
| アプリケーション: | WB |
| 反応性: | Human |
| 分子量: | 60 kDa; 57kD(Calculated). |
| ユニプロット: | Q96T60 |
| RRID: | AB_2840557 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF8503, RRID:AB_2840557.
折りたたみ／展開
2''(3'')-polynucleotidase; 2'(3')-polynucleotidase; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; DNA 5''-kinase/3''-phosphatase; EIEE10; Homo sapiens polynucleotide kinase 3' phosphatase (PNKP); MCSZ; PNK 1; PNK1; Pnkp; PNKP DNA kinase; PNKP_HUMAN; Polynucleotide 3'-phosphatase; Polynucleotide 5' hydroxyl kinase; Polynucleotide 5''-hydroxyl-kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; Polynucleotide kinase-3''-phosphatase;
免疫原
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine (PubMed:10446192). Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver (PubMed:10446193).
- Q96T60 PNKP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVNPSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRMRKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEGYKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPISIGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSGPLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPTLAEGFSAILEIPFRLWVEPRLGRLYCQFSEG
PTMs - Q96T60 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| T111 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Q13315 (ATM) , P78527 (PRKDC) | Uniprot |
| T118 | Phosphorylation | Uniprot | |
| T122 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | P78527 (PRKDC) , Q13315 (ATM) | Uniprot |
| K142 | Ubiquitination | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| K183 | Acetylation | Uniprot | |
| K183 | Sumoylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| R224 | Methylation | Uniprot | |
| K226 | Acetylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S379 | Phosphorylation | Uniprot | |
| K484 | Ubiquitination | Uniprot |
研究背景
Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.
Nucleus.
Expressed in many tissues with highest expression in spleen and testis, and lowest expression in small intestine. Expressed in higher amount in pancreas, heart and kidney and at lower levels in brain, lung and liver.
Monomer.
The FHA domain binds threonine-phosphorylated peptides from XRCC1/4, and is responsible for the recruitment of PNKP to the sites of DNA repair. The affinity is ten times greater if peptides are also phosphorylated on the serine preceeding the phosphothreonine.
In the N-terminal section; belongs to the DNA 3' phosphatase family.
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