Phospho-TRPV1(Ser502) Antibody - #AF8520

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製品: Phospho-TRPV1(Ser502) Antibody
カタログ: AF8520
タンパク質の説明: Rabbit polyclonal antibody to Phospho-TRPV1(Ser502)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 95 kDa; 95kD(Calculated).
ユニプロット: Q8NER1
RRID: AB_2840574

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Bovine(91%), Sheep(91%), Rabbit(82%), Dog(91%), Chicken(82%), Xenopus(82%)
クローナリティ:
Polyclonal
特異性:
Phospho-VR1 (Ser502) Antibody detects endogenous levels of VR1 only when phosphorylated at Ser502.
RRID:
AB_2840574
引用形式: Affinity Biosciences Cat# AF8520, RRID:AB_2840574.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Capsaicin receptor; DKFZp434K0220; osm 9 like TRP channel 1; Osm-9-like TRP channel 1; OTRPC1; Transient receptor potential cation channel subfamily V member 1; TRPV 1; Trpv1; TRPV1_HUMAN; Vanilloid receptor 1; Vanilloid receptor subtype 1; VR 1; VR1;

免疫原

免疫原:
Uniprot:

Q8NER1(TRPV1_HUMAN) >>Visit HPA database.

遺伝子(ID):
TRPV1(7442)
発現特異性:
Q8NER1 TRPV1_HUMAN:

Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).

タンパク質配列:
MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFPVDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLVENGADVQAAAHGDFFKKTKGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISARDSVGNTVLHALVEVADNTADNTKFVTSMYNEILMLGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVLAYILQREIQEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLNRLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEKTGDYFRVTGEILSVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVASMVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYIVFLFGFSTAVVTLIEDGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRKAFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLRSSRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
91
Xenopus
82
Chicken
82
Rabbit
82
Zebrafish
67
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NER1 基板として

Site PTM Type Enzyme
S5 Phosphorylation
S6 Phosphorylation
T7 Phosphorylation
S117 Phosphorylation Q15139 (PRKD1)
S132 Phosphorylation
S140 Phosphorylation
K161 Acetylation
T196 Phosphorylation
Y200 Phosphorylation P12931 (SRC)
T336 Phosphorylation
T407 Phosphorylation
S502 Phosphorylation Q02156 (PRKCE) , P17612 (PRKACA) , P17252 (PRKCA)
T705 Phosphorylation P05771-2 (PRKCB)
T773 Phosphorylation
S775 Phosphorylation
S780 Phosphorylation
S801 Phosphorylation Q02156 (PRKCE) , P17252 (PRKCA)
S807 Phosphorylation
Y814 Phosphorylation

研究背景

機能:

Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.

PTMs:

Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.

細胞の位置付け:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell projection>Dendritic spine membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Mostly, but not exclusively expressed in postsynaptic dendritic spines.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).

サブユニット構造:

Interacts with PIRT (By similarity). Homotetramer (By similarity). Interacts with TRPV3 and may also form a heteromeric channel with TRPV3. Interacts with CALM, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity). Interacts with the Scolopendra mutilans RhTx toxin (By similarity). Interacts with TMEM100 (By similarity). Interacts with PACS2.

タンパク質ファミリー:

The association domain (AD) is necessary for self-association.

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

参考文献

1). Selective activation of AKAP150/TRPV1 in ventrolateral periaqueductal gray GABAergic neurons facilitates conditioned place aversion in male mice. Communications Biology, 2023 (PubMed: 37460788) [IF=5.9]

Application: WB    Species: Mouse    Sample:

Fig. 5 TRPV1 in GABAergic neurons of the vlPAG in CPA mice. Expression of TRPV1 in the pinch pain CPA model (a) and morphine withdrawal CPA model (b). (two sample t test, n = 6). p-TRPV1 was upregulated in the pinch pain CPA model (c, d) and morphine withdrawal CPA model (e, f) [immunofluorescence image (c, e), scale bar, 50 μm (n = 3); western blotting (d, f), ***P 

Application: IF/ICC    Species: Mouse    Sample:

Fig. 5 TRPV1 in GABAergic neurons of the vlPAG in CPA mice. Expression of TRPV1 in the pinch pain CPA model (a) and morphine withdrawal CPA model (b). (two sample t test, n = 6). p-TRPV1 was upregulated in the pinch pain CPA model (c, d) and morphine withdrawal CPA model (e, f) [immunofluorescence image (c, e), scale bar, 50 μm (n = 3); western blotting (d, f), ***P 
2). Pingchong Jiangni recipe through nerve growth factor/transient receptor potential vanilloid 1 signaling pathway to relieve pain in endometriosis model rats. Journal of Ethnopharmacology, 2024 (PubMed: 37479067) [IF=5.4]
3). Esketamine alleviates hypoxia/reoxygenation injury of cardiomyocytes by regulating TRPV1 expression and inhibiting intracellular Ca2+ concentration. Clinics (Sao Paulo, Brazil), 2024 (PubMed: 38692008) [IF=2.7]
4). Activation of Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) with Lidocaine Provokes Pyroptosis of Glioblastoma Cells. BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE, 2021 (PubMed: 34302204) [IF=0.7]

Application: WB    Species: Human    Sample: U87-MG cells

Fig. 1. LA activated TRPV1 through CaMKII to induce Ca 2+ overload. a) CaMKII content in U87-MG cells (Western blotting): 1) negative control (NC); 2) CaMKII-silencing (CaMKIIA); b) phosphorylation of TRPV1 in U87-MG cells (Western blotting): 1) NC; 2) NC+lidocaine; 3) CaMKIIA+lidocaine. c) Effect of different concentrations of lidocaine on intracellular Ca 2+ concentration in control U87-MG cells and cells transfected with si-CaMKII (CaMKIIA) (flow cytometry). p<0.05 in comparison with *NC, + CaMKIIA+lidocaine.

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