VCAN Antibody - #DF10007
(3)
(5)
| 製品: | VCAN Antibody |
| カタログ: | DF10007 |
| タンパク質の説明: | Rabbit polyclonal antibody to VCAN |
| アプリケーション: | WB IHC |
| 反応性: | Human |
| 予測: | Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 373 kDa; 373kD(Calculated). |
| ユニプロット: | P13611 |
| RRID: | AB_2840587 |
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human
予測:
Bovine(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
VCAN Antibody detects endogenous levels of total VCAN.
RRID:
AB_2840587
引用形式: Affinity Biosciences Cat# DF10007, RRID:AB_2840587.
引用形式: Affinity Biosciences Cat# DF10007, RRID:AB_2840587.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
Chondroitin sulfate proteoglycan 2; Chondroitin sulfate proteoglycan core protein 2; Chondroitin sulfate proteoglycan core protein, cartilage; CSPG2; CSPG2_HUMAN; ERVR; GHAP; Glial hyaluronate binding protein; Glial hyaluronate-binding protein; Large fibroblast proteoglycan; PG-M; PGM; VCAN; Versican; Versican core protein; Versican proteoglycan; WGN 1; WGN; WGN1;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P13611 CSPG2_HUMAN:
Expressed in the retina (at protein level) (PubMed:29777959). Cerebral white matter and plasma (PubMed:2469524). Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas (PubMed:8627343). Isoform V2: Restricted to normal brain and gliomas (PubMed:8627343). Isoform V3: Found in all these tissues except medulloblastomas (PubMed:8627343).
タンパク質配列:
- P13611 CSPG2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFINIKSILWMCSTLIVTHALHKVKVGKSPPVRGSLSGKVSLPCHFSTMPTLPPSYNTSEFLRIKWSKIEVDKNGKDLKETTVLVAQNGNIKIGQDYKGRVSVPTHPEAVGDASLTVVKLLASDAGLYRCDVMYGIEDTQDTVSLTVDGVVFHYRAATSRYTLNFEAAQKACLDVGAVIATPEQLFAAYEDGFEQCDAGWLADQTVRYPIRAPRVGCYGDKMGKAGVRTYGFRSPQETYDVYCYVDHLDGDVFHLTVPSKFTFEEAAKECENQDARLATVGELQAAWRNGFDQCDYGWLSDASVRHPVTVARAQCGGGLLGVRTLYRFENQTGFPPPDSRFDAYCFKPKEATTIDLSILAETASPSLSKEPQMVSDRTTPIIPLVDELPVIPTEFPPVGNIVSFEQKATVQPQAITDSLATKLPTPTGSTKKPWDMDDYSPSASGPLGKLDISEIKEEVLQSTTGVSHYATDSWDGVVEDKQTQESVTQIEQIEVGPLVTSMEILKHIPSKEFPVTETPLVTARMILESKTEKKMVSTVSELVTTGHYGFTLGEEDDEDRTLTVGSDESTLIFDQIPEVITVSKTSEDTIHTHLEDLESVSASTTVSPLIMPDNNGSSMDDWEERQTSGRITEEFLGKYLSTTPFPSQHRTEIELFPYSGDKILVEGISTVIYPSLQTEMTHRRERTETLIPEMRTDTYTDEIQEEITKSPFMGKTEEEVFSGMKLSTSLSEPIHVTESSVEMTKSFDFPTLITKLSAEPTEVRDMEEDFTATPGTTKYDENITTVLLAHGTLSVEAATVSKWSWDEDNTTSKPLESTEPSASSKLPPALLTTVGMNGKDKDIPSFTEDGADEFTLIPDSTQKQLEEVTDEDIAAHGKFTIRFQPTTSTGIAEKSTLRDSTTEEKVPPITSTEGQVYATMEGSALGEVEDVDLSKPVSTVPQFAHTSEVEGLAFVSYSSTQEPTTYVDSSHTIPLSVIPKTDWGVLVPSVPSEDEVLGEPSQDILVIDQTRLEATISPETMRTTKITEGTTQEEFPWKEQTAEKPVPALSSTAWTPKEAVTPLDEQEGDGSAYTVSEDELLTGSERVPVLETTPVGKIDHSVSYPPGAVTEHKVKTDEVVTLTPRIGPKVSLSPGPEQKYETEGSSTTGFTSSLSPFSTHITQLMEETTTEKTSLEDIDLGSGLFEKPKATELIEFSTIKVTVPSDITTAFSSVDRLHTTSAFKPSSAITKKPPLIDREPGEETTSDMVIIGESTSHVPPTTLEDIVAKETETDIDREYFTTSSPPATQPTRPPTVEDKEAFGPQALSTPQPPASTKFHPDINVYIIEVRENKTGRMSDLSVIGHPIDSESKEDEPCSEETDPVHDLMAEILPEFPDIIEIDLYHSEENEEEEEECANATDVTTTPSVQYINGKHLVTTVPKDPEAAEARRGQFESVAPSQNFSDSSESDTHPFVIAKTELSTAVQPNESTETTESLEVTWKPETYPETSEHFSGGEPDVFPTVPFHEEFESGTAKKGAESVTERDTEVGHQAHEHTEPVSLFPEESSGEIAIDQESQKIAFARATEVTFGEEVEKSTSVTYTPTIVPSSASAYVSEEEAVTLIGNPWPDDLLSTKESWVEATPRQVVELSGSSSIPITEGSGEAEEDEDTMFTMVTDLSQRNTTDTLITLDTSRIITESFFEVPATTIYPVSEQPSAKVVPTKFVSETDTSEWISSTTVEEKKRKEEEGTTGTASTFEVYSSTQRSDQLILPFELESPNVATSSDSGTRKSFMSLTTPTQSEREMTDSTPVFTETNTLENLGAQTTEHSSIHQPGVQEGLTTLPRSPASVFMEQGSGEAAADPETTTVSSFSLNVEYAIQAEKEVAGTLSPHVETTFSTEPTGLVLSTVMDRVVAENITQTSREIVISERLGEPNYGAEIRGFSTGFPLEEDFSGDFREYSTVSHPIAKEETVMMEGSGDAAFRDTQTSPSTVPTSVHISHISDSEGPSSTMVSTSAFPWEEFTSSAEGSGEQLVTVSSSVVPVLPSAVQKFSGTASSIIDEGLGEVGTVNEIDRRSTILPTAEVEGTKAPVEKEEVKVSGTVSTNFPQTIEPAKLWSRQEVNPVRQEIESETTSEEQIQEEKSFESPQNSPATEQTIFDSQTFTETELKTTDYSVLTTKKTYSDDKEMKEEDTSLVNMSTPDPDANGLESYTTLPEATEKSHFFLATALVTESIPAEHVVTDSPIKKEESTKHFPKGMRPTIQESDTELLFSGLGSGEEVLPTLPTESVNFTEVEQINNTLYPHTSQVESTSSDKIEDFNRMENVAKEVGPLVSQTDIFEGSGSVTSTTLIEILSDTGAEGPTVAPLPFSTDIGHPQNQTVRWAEEIQTSRPQTITEQDSNKNSSTAEINETTTSSTDFLARAYGFEMAKEFVTSAPKPSDLYYEPSGEGSGEVDIVDSFHTSATTQATRQESSTTFVSDGSLEKHPEVPSAKAVTADGFPTVSVMLPLHSEQNKSSPDPTSTLSNTVSYERSTDGSFQDRFREFEDSTLKPNRKKPTENIIIDLDKEDKDLILTITESTILEILPELTSDKNTIIDIDHTKPVYEDILGMQTDIDTEVPSEPHDSNDESNDDSTQVQEIYEAAVNLSLTEETFEGSADVLASYTQATHDESMTYEDRSQLDHMGFHFTTGIPAPSTETELDVLLPTATSLPIPRKSATVIPEIEGIKAEAKALDDMFESSTLSDGQAIADQSEIIPTLGQFERTQEEYEDKKHAGPSFQPEFSSGAEEALVDHTPYLSIATTHLMDQSVTEVPDVMEGSNPPYYTDTTLAVSTFAKLSSQTPSSPLTIYSGSEASGHTEIPQPSALPGIDVGSSVMSPQDSFKEIHVNIEATFKPSSEEYLHITEPPSLSPDTKLEPSEDDGKPELLEEMEASPTELIAVEGTEILQDFQNKTDGQVSGEAIKMFPTIKTPEAGTVITTADEIELEGATQWPHSTSASATYGVEAGVVPWLSPQTSERPTLSSSPEINPETQAALIRGQDSTIAASEQQVAARILDSNDQATVNPVEFNTEVATPPFSLLETSNETDFLIGINEESVEGTAIYLPGPDRCKMNPCLNGGTCYPTETSYVCTCVPGYSGDQCELDFDECHSNPCRNGATCVDGFNTFRCLCLPSYVGALCEQDTETCDYGWHKFQGQCYKYFAHRRTWDAAERECRLQGAHLTSILSHEEQMFVNRVGHDYQWIGLNDKMFEHDFRWTDGSTLQYENWRPNQPDSFFSAGEDCVVIIWHENGQWNDVPCNYHLTYTCKKGTVACGQPPVVENAKTFGKMKPRYEINSLIRYHCKDGFIQRHLPTIRCLGNGRWAIPKITCMNPSAYQRTYSMKYFKNSSSAKDNSINTSKHDHRWSRRWQESRR
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Bovine
91
Sheep
91
Pig
0
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P13611 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K68 | Acetylation | Uniprot | |
| K79 | Acetylation | Uniprot | |
| K98 | Acetylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S510 | O-Glycosylation | Uniprot | |
| T516 | O-Glycosylation | Uniprot | |
| T518 | O-Glycosylation | Uniprot | |
| T522 | O-Glycosylation | Uniprot | |
| T627 | O-Glycosylation | Uniprot | |
| T632 | O-Glycosylation | Uniprot | |
| S641 | O-Glycosylation | Uniprot | |
| T642 | O-Glycosylation | Uniprot | |
| T642 | Phosphorylation | Uniprot | |
| T643 | O-Glycosylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| T687 | O-Glycosylation | Uniprot | |
| T689 | O-Glycosylation | Uniprot | |
| T716 | O-Glycosylation | Uniprot | |
| T716 | Phosphorylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| T751 | O-Glycosylation | Uniprot | |
| T754 | O-Glycosylation | Uniprot | |
| T792 | O-Glycosylation | Uniprot | |
| T799 | O-Glycosylation | Uniprot | |
| S801 | O-Glycosylation | Uniprot | |
| T869 | O-Glycosylation | Uniprot | |
| T886 | O-Glycosylation | Uniprot | |
| T887 | O-Glycosylation | Uniprot | |
| S888 | O-Glycosylation | Uniprot | |
| T889 | O-Glycosylation | Uniprot | |
| S976 | O-Glycosylation | Uniprot | |
| T981 | O-Glycosylation | Uniprot | |
| T1092 | O-Glycosylation | Uniprot | |
| T1093 | O-Glycosylation | Uniprot | |
| S1101 | O-Glycosylation | Uniprot | |
| S1103 | O-Glycosylation | Uniprot | |
| T1110 | O-Glycosylation | Uniprot | |
| T1116 | O-Glycosylation | Uniprot | |
| T1121 | O-Glycosylation | Uniprot | |
| T1123 | O-Glycosylation | Uniprot | |
| S1131 | O-Glycosylation | Uniprot | |
| S1133 | O-Glycosylation | Uniprot | |
| T1191 | O-Glycosylation | Uniprot | |
| S1212 | O-Glycosylation | Uniprot | |
| T1219 | O-Glycosylation | Uniprot | |
| T1220 | O-Glycosylation | Uniprot | |
| S1221 | O-Glycosylation | Uniprot | |
| S1349 | Phosphorylation | Uniprot | |
| S1351 | Phosphorylation | Uniprot | |
| T1418 | O-Glycosylation | Uniprot | |
| T1419 | O-Glycosylation | Uniprot | |
| T1566 | O-Glycosylation | Uniprot | |
| T1569 | O-Glycosylation | Uniprot | |
| S1716 | O-Glycosylation | Uniprot | |
| T1719 | O-Glycosylation | Uniprot | |
| T1731 | O-Glycosylation | Uniprot | |
| T1734 | O-Glycosylation | Uniprot | |
| S1742 | O-Glycosylation | Uniprot | |
| S1743 | O-Glycosylation | Uniprot | |
| T1744 | O-Glycosylation | Uniprot | |
| T1763 | O-Glycosylation | Uniprot | |
| S1765 | O-Glycosylation | Uniprot | |
| T1769 | O-Glycosylation | Uniprot | |
| S1772 | O-Glycosylation | Uniprot | |
| S1775 | O-Glycosylation | Uniprot | |
| T1777 | O-Glycosylation | Uniprot | |
| T1778 | O-Glycosylation | Uniprot | |
| T1780 | O-Glycosylation | Uniprot | |
| S1909 | O-Glycosylation | Uniprot | |
| S1925 | O-Glycosylation | Uniprot | |
| T1926 | O-Glycosylation | Uniprot | |
| S1935 | O-Glycosylation | Uniprot | |
| S1942 | O-Glycosylation | Uniprot | |
| T1943 | O-Glycosylation | Uniprot | |
| S1945 | O-Glycosylation | Uniprot | |
| T2036 | O-Glycosylation | Uniprot | |
| T2050 | Phosphorylation | Uniprot | |
| S2058 | O-Glycosylation | Uniprot | |
| S2058 | Phosphorylation | Uniprot | |
| T2059 | O-Glycosylation | Uniprot | |
| T2059 | Phosphorylation | Uniprot | |
| T2063 | O-Glycosylation | Uniprot | |
| T2069 | O-Glycosylation | Uniprot | |
| T2083 | O-Glycosylation | Uniprot | |
| S2085 | O-Glycosylation | Uniprot | |
| T2086 | O-Glycosylation | Uniprot | |
| T2086 | Phosphorylation | Uniprot | |
| T2091 | O-Glycosylation | Uniprot | |
| S2099 | Phosphorylation | Uniprot | |
| T2115 | Phosphorylation | Uniprot | |
| S2116 | Phosphorylation | Uniprot | |
| T2152 | O-Glycosylation | Uniprot | |
| T2153 | O-Glycosylation | Uniprot | |
| T2159 | O-Glycosylation | Uniprot | |
| T2160 | O-Glycosylation | Uniprot | |
| T2163 | Phosphorylation | Uniprot | |
| T2194 | O-Glycosylation | Uniprot | |
| T2200 | O-Glycosylation | Uniprot | |
| S2203 | O-Glycosylation | Uniprot | |
| K2238 | Methylation | Uniprot | |
| T2331 | Phosphorylation | Uniprot | |
| S2337 | Phosphorylation | Uniprot | |
| T2416 | O-Glycosylation | Uniprot | |
| S2417 | O-Glycosylation | Uniprot | |
| S2422 | O-Glycosylation | Uniprot | |
| T2448 | O-Glycosylation | Uniprot | |
| S2455 | O-Glycosylation | Uniprot | |
| S2456 | O-Glycosylation | Uniprot | |
| T2457 | O-Glycosylation | Uniprot | |
| T2458 | O-Glycosylation | Uniprot | |
| S2473 | O-Glycosylation | Uniprot | |
| T2503 | O-Glycosylation | Uniprot | |
| T2505 | O-Glycosylation | Uniprot | |
| T2509 | O-Glycosylation | Uniprot | |
| S2515 | O-Glycosylation | Uniprot | |
| T2516 | O-Glycosylation | Uniprot | |
| S2530 | O-Glycosylation | Uniprot | |
| T2531 | O-Glycosylation | Uniprot | |
| T2540 | O-Glycosylation | Uniprot | |
| T2617 | Phosphorylation | Uniprot | |
| T2656 | O-Glycosylation | Uniprot | |
| S2661 | O-Glycosylation | Uniprot | |
| S2699 | O-Glycosylation | Uniprot | |
| T2701 | O-Glycosylation | Uniprot | |
| S2781 | O-Glycosylation | Uniprot | |
| T2784 | O-Glycosylation | Uniprot | |
| T2785 | O-Glycosylation | Uniprot | |
| S2941 | Phosphorylation | Uniprot | |
| T2950 | O-Glycosylation | Uniprot | |
| S3024 | O-Glycosylation | Uniprot | |
| T3025 | O-Glycosylation | Uniprot | |
| S3029 | O-Glycosylation | Uniprot | |
| T3205 | Phosphorylation | Uniprot | |
| S3206 | Phosphorylation | Uniprot | |
| S3209 | Phosphorylation | Uniprot | |
| K3312 | Acetylation | Uniprot | |
| S3363 | Phosphorylation | Uniprot |
研究背景
機能:
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
PTMs:
Phosphorylated by FAM20C in the extracellular medium.
細胞の位置付け:
Secreted>Extracellular space>Extracellular matrix. Cell projection>Cilium>Photoreceptor outer segment. Secreted>Extracellular space>Extracellular matrix>Interphotoreceptor matrix.
組織特異性:
Expressed in the retina (at protein level). Cerebral white matter and plasma. Isoform V0: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V1: Expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2: Restricted to normal brain and gliomas. Isoform V3: Found in all these tissues except medulloblastomas.
サブユニット構造:
Interacts with FBLN1.
タンパク質ファミリー:
Belongs to the aggrecan/versican proteoglycan family.
研究領域
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
参考文献
1). Establishment and functional characterization of immortalized rabbit dermal papilla cell lines. Animal biotechnology, 2023
(PubMed: 37652434)
[IF=3.7]
Application: IF/ICC Species: rabbit Sample: DPCs and R-7 cell
Figure 2. Indirect immunofluorescence staining of primary DPCs and R-7. All the DPCs were incubated with antibodies against α-SMA, SV40LT and Versican (Scale Bar: 50 µm). Passage 5 of DPCs were used.
2). miR-543 impairs cell proliferation, migration, and invasion in breast cancer by suppressing VCAN. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2021
(PubMed: 34293593)
[IF=3.1]
Application: WB Species: Human Sample: BC cell
Fig. 4. The low expression of VCAN blocked BC cell malignancy caused by miR-543 inhibitor. (A) VCAN protein levels in MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-
543 inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by western blotting. (B) Cell viability of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543
inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by CCK-8 assay. (C) Cell proliferation of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543
inhibitor, miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC were detected by BrdU assay. (D) Cell migration of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor,
miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC was detected by wound healing assay. (E) Cell invasion of MDA-MB-231 and MCF-7 treated with si-VCAN þ miR-543 inhibitor,
miR-543 inhibitor, si-VCAN, inhibitor-NC and si-NC was detected by transwell assay. *P < 0.05, **P < 0.001 versus negative control group. #P < 0.05, ##P < 0.001 versus si-
VCAN þ miR-543 inhibitor. Data are presented as the mean ± SD.
3). Exosomes Derived from Dermal Papilla Cells Mediate Hair Follicle Stem Cell Proliferation through the Wnt3a/β-Catenin Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2022
(PubMed: 36388171)
Application: IF/ICC Species: Mouse Sample:
Figure 1 Morphology and indirect immunofluorescence staining of HFSCs and DPCs. (a) α-SMA and Versican antibodies were incubated with DPCs (passage 2) (scale bar: 50 μm). (b) Alkaline phosphatase staining was examined with DPCs (passage 8) (scale bar: 100 μm, 50 μm). (c) KRT14, KRT15, and KRT19 antibodies were incubated with HFSCs (passage 2) (scale bar: 50 μm).
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