CYFIP1 Antibody - #DF10059
| 製品: | CYFIP1 Antibody |
| カタログ: | DF10059 |
| タンパク質の説明: | Rabbit polyclonal antibody to CYFIP1 |
| アプリケーション: | WB |
| 反応性: | Human |
| 予測: | Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 145 kDa; 145kD(Calculated). |
| ユニプロット: | Q7L576 |
| RRID: | AB_2840639 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF10059, RRID:AB_2840639.
折りたたみ／展開
CY; CYFIP 1; CYFIP1; CYFP1_HUMAN; Cytoplasmic FMR1 interacting protein 1; Cytoplasmic FMR1-interacting protein 1; FLJ45151; KIAA0068; p140sra 1; p140sra-1; P140SRA1; Selective hybridizing clone; SHYC; Specifically Rac1 associated protein 1; Specifically Rac1-associated protein 1; SRA 1; Sra-1; SRA1;
免疫原
- Q7L576 CYFP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAQVTLEDALSNVDLLEELPLPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMNFMYFQRNAIERFCGEVRRLCHAERRKDFVSEAYLITLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNKITQSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGLYLMDGSVSNIYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCTSSGSSPQYNICEQMIQIREDHMRFISELARYSNSEVVTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPTDKYSNKDCPDSAEEYERATRYNYTSEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTVYAALQDFSQVTLREPLRQAIKKKKNVIQSVLQAIRKTVCDWETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTRFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQRVSAAMYKSLELAIGRFESEDLTSIVELDGLLEINRMTHKLLSRYLTLDGFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAILFCLLIEQSLSLEEVCDLLHAAPFQNILPRVHVKEGERLDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRSFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGEGTPVEHVRCFQPPIHQSLASS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q7L576 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K97 | Ubiquitination | Uniprot | |
| Y108 | Phosphorylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| K182 | Acetylation | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K189 | Acetylation | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| Y254 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| Y366 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| T420 | Phosphorylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K540 | Ubiquitination | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| Y560 | Phosphorylation | Uniprot | |
| S568 | Phosphorylation | Uniprot | |
| K573 | Ubiquitination | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| T579 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S583 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| K751 | Ubiquitination | Uniprot | |
| T769 | Phosphorylation | Uniprot | |
| K778 | Ubiquitination | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| K879 | Ubiquitination | Uniprot | |
| Y887 | Phosphorylation | Uniprot | |
| S900 | Phosphorylation | Uniprot | |
| K935 | Ubiquitination | Uniprot | |
| Y967 | Phosphorylation | Uniprot | |
| Y986 | Phosphorylation | Uniprot | |
| K1038 | Ubiquitination | Uniprot | |
| S1052 | Phosphorylation | Uniprot | |
| K1053 | Ubiquitination | Uniprot | |
| Y1054 | Phosphorylation | Uniprot | |
| T1068 | Phosphorylation | Uniprot | |
| K1083 | Ubiquitination | Uniprot | |
| K1188 | Ubiquitination | Uniprot | |
| K1197 | Acetylation | Uniprot | |
| K1197 | Ubiquitination | Uniprot | |
| K1210 | Ubiquitination | Uniprot | |
| K1227 | Ubiquitination | Uniprot | |
| T1234 | Phosphorylation | Uniprot | |
| S1253 | Phosphorylation | Uniprot |
研究背景
Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). May act as an invasion suppressor in cancers.
Cytoplasm. Cytoplasm>Perinuclear region. Cell projection>Lamellipodium. Cell projection>Ruffle. Cell junction>Synapse>Synaptosome.
Note: Highly expressed in the perinuclear region (By similarity). Enriched in synaptosomes (By similarity). Also enriched in membrane ruffles and at the tips of lamellipodia (By similarity).
Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with FMR1 but does not bind to related proteins FXR1 or FXR2. Interaction with EIF4E stimulates FMR1 binding. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Interacts with the active GTP-bound form of RAC1. Interacts through its C-terminus with the C-terminus of DPYSL2/CRMP2 which is necessary for DPYSL2-induced axon outgrowth. Interacts with NYAP1, NYAP2 and MYO16. Interacts with TMEM108 (via N-terminus); the interaction associates TMEM108 with the WAVE1 complex (By similarity).
Belongs to the CYFIP family.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
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