HIPK3 Antibody - #DF10079
| 製品: | HIPK3 Antibody |
| カタログ: | DF10079 |
| タンパク質の説明: | Rabbit polyclonal antibody to HIPK3 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 134 kDa; 134kD(Calculated). |
| ユニプロット: | Q9H422 |
| RRID: | AB_2840659 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF10079, RRID:AB_2840659.
折りたたみ／展開
Androgen receptor interacting nuclear protein kinase; Androgen receptor-interacting nuclear protein kinase; ANPK; DYRK6; Fas interacting serine/threonine protein kinase; Fas-interacting serine/threonine-protein kinase; FIST; FIST3; Hipk3; HIPK3_HUMAN; homeodomain interacting protein kinase 3; Homeodomain-interacting protein kinase 3; Homolog of protein kinase YAK1; PKY; RP24 297H17.5; YAK1;
免疫原
Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
- Q9H422 HIPK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASQVLVYPPYVYQTQSSAFCSVKKLKVEPSSCVFQERNYPRTYVNGRNFGNSHPPTKGSAFQTKIPFNRPRGHNFSLQTSAVVLKNTAGATKVIAAQAQQAHVQAPQIGAWRNRLHFLEGPQRCGLKRKSEELDNHSSAMQIVDELSILPAMLQTNMGNPVTVVTATTGSKQNCTTGEGDYQLVQHEVLCSMKNTYEVLDFLGRGTFGQVVKCWKRGTNEIVAIKILKNHPSYARQGQIEVSILARLSTENADEYNFVRAYECFQHRNHTCLVFEMLEQNLYDFLKQNKFSPLPLKVIRPILQQVATALKKLKSLGLIHADLKPENIMLVDPVRQPYRVKVIDFGSASHVSKTVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGALEYDQIRYISQTQGLPGEQLLNVGTKSTRFFCKETDMSHSGWRLKTLEEHEAETGMKSKEARKYIFNSLDDVAHVNTVMDLEGSDLLAEKADRREFVSLLKKMLLIDADLRITPAETLNHPFVNMKHLLDFPHSNHVKSCFHIMDICKSHLNSCDTNNHNKTSLLRPVASSSTATLTANFTKIGTLRSQALTTSAHSVVHHGIPLQAGTAQFGCGDAFQQTLIICPPAIQGIPATHGKPTSYSIRVDNTVPLVTQAPAVQPLQIRPGVLSQTWSGRTQQMLVPAWQQVTPLAPATTTLTSESVAGSHRLGDWGKMISCSNHYNSVMPQPLLTNQITLSAPQPVSVGIAHVVWPQPATTKKNKQCQNRGILVKLMEWEPGREEINAFSWSNSLQNTNIPHSAFISPKIINGKDVEEVSCIETQDNQNSEGEARNCCETSIRQDSDSSVSDKQRQTIIIADSPSPAVSVITISSDTDEEETSQRHSLRECKGSLDCEACQSTLNIDRMCSLSSPDSTLSTSSSGQSSPSPCKRPNSMSDEEQESSCDTVDGSPTSDSSGHDSPFAESTFVEDTHENTELVSSADTETKPAVCSVVVPPVELENGLNADEHMANTDSICQPLIKGRSAPGRLNQPSAVGTRQQKLTSAFQQQHLNFSQVQHFGSGHQEWNGNFGHRRQQAYIPTSVTSNPFTLSHGSPNHTAVHAHLAGNTHLGGQPTLLPYPSSATLSSAAPVAHLLASPCTSRPMLQHPTYNISHPSGIVHQVPVGLNPRLLPSPTIHQTQYKPIFPPHSYIAASPAYTGFPLSPTKLSQYPYM
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H422 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y8 | Phosphorylation | Uniprot | |
| K27 | Sumoylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| T219 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K229 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K459 | Ubiquitination | Uniprot | |
| Y466 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| T515 | Phosphorylation | Uniprot | |
| T519 | Phosphorylation | Uniprot | |
| K528 | Methylation | Uniprot | |
| K528 | Ubiquitination | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| K813 | Ubiquitination | Uniprot | |
| K852 | Ubiquitination | Uniprot | |
| K891 | Ubiquitination | Uniprot | |
| S1205 | Phosphorylation | Uniprot |
研究背景
Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation: phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
Autophosphorylated, but autophosphorylation is not required for catalytic activity.
May be sumoylated.
Cytoplasm. Nucleus.
Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung.
Interacts with Nkx1-2. Interacts with FAS and DAXX. Probably part of a complex consisting of HIPK3, FAS and FADD. Interacts with and stabilizes ligand-bound androgen receptor (AR) (By similarity). Interacts with UBL1/SUMO-1. Binds to NR5A1/SF1, SPEN/MINT and RUNX2.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
研究領域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
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