製品: DNAJB2 Antibody
カタログ: DF10101
タンパク質の説明: Rabbit polyclonal antibody to DNAJB2
アプリケーション: WB
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 36 kDa; 36kD(Calculated).
ユニプロット: P25686
RRID: AB_2840681

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Dog(89%)
クローナリティ:
Polyclonal
特異性:
DNAJB2 Antibody detects endogenous levels of total DNAJB2.
RRID:
AB_2840681
引用形式: Affinity Biosciences Cat# DF10101, RRID:AB_2840681.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Descriptions; DnaJ (Hsp40) homolog subfamily B member 2; DnaJ homolog subfamily B member 2; DnaJ protein homolog 1; DNAJB2; Heat shock 40 kDa protein 3; Heat shock protein J1; Heat shock protein neuronal DNAJ like 1; HSJ 1; HSJ1; HSPF3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P25686 DNJB2_HUMAN:

More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level) (PubMed:1599432, PubMed:12754272). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis (at protein level) (PubMed:12754272, PubMed:1599432). Isoform 1 is more abundant in neocortex and cerebellum compared to isoform 2 (at protein level) (PubMed:12754272).

タンパク質配列:
MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPAGGREAQHRRQGRPKAQHQDPGLGGTQEGARGEATKRSPSPEEKASRCLIL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
89
Rabbit
71
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P25686 基板として

Site PTM Type Enzyme
S3 Phosphorylation
K20 Ubiquitination
K21 Ubiquitination
K26 Ubiquitination
K34 Ubiquitination
K39 Ubiquitination
K45 Ubiquitination
K47 Ubiquitination
K59 Ubiquitination
K61 Ubiquitination
Y65 Phosphorylation
T76 Phosphorylation
T78 Phosphorylation
S87 Phosphorylation
T95 Phosphorylation
S168 Phosphorylation
S202 Phosphorylation
T204 Phosphorylation
S219 Phosphorylation
T239 Phosphorylation
S247 Phosphorylation
S250 Phosphorylation
Y261 Phosphorylation
S264 Phosphorylation
K288 Methylation
K288 Ubiquitination
T308 Phosphorylation
S311 Phosphorylation
S313 Phosphorylation
C321 S-Nitrosylation

研究背景

機能:

Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family. In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins. Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes. Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins.

PTMs:

Ubiquitinated by STUB1; does not lead to proteasomal degradation.

細胞の位置付け:

Cytoplasm. Nucleus.

Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

More abundantly expressed in neocortex, cerebellum, spinal cord and retina where it is expressed by neuronal cells (at protein level). Detected at much lower level in non-neuronal tissues including kidney, lung, heart, skeletal muscle, spleen and testis (at protein level). Isoform 1 is more abundant in neocortex and cerebellum compared to isoform 2 (at protein level).

サブユニット構造:

Interacts with HSP70 (HSPA1A or HSPA1B). Interacts with HSPA8/Hsc70. Interacts with PSMA3 and most probably with the whole proteasomal complex.

タンパク質ファミリー:

The J domain is sufficient to interact with HSP70 (HSPA1A or HSPA1B) and activate its ATPase activity (PubMed:22219199). The J domain is also required for the HSP70-mediated and ubiquitin-dependent proteasomal degradation of proteins like ATXN3 (PubMed:21625540). The J domain is required to reduce PRKN cytoplasmic aggregation (PubMed:20889486).

The UIM domains mediate interaction with ubiquitinated chaperone clients and with the proteasome (PubMed:15936278). The UIM domains may have an opposite activity to the J domain, binding ubiquitinated proteins and protecting them from HSP70-mediated proteasomal degradation (PubMed:21625540). The UIM domains are not required to reduce PRKN cytoplasmic aggregation (PubMed:20889486).

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.