CDC14B/C Antibody - #DF10121
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF10121, RRID:AB_2840701.
折りたたみ／展開
CC14B_HUMAN; Cdc 14B; Cdc 14B1; Cdc 14B2; CDC 14B3; CDC14 cell division cycle 14 homolog B; CDC14 homolog B; Cdc14b; Cdc14B1; Cdc14B2; CDC14B3; Cell division cycle 14 homolog B; Dual specificity protein phosphatase CDC14B; hCDC 14B; hCDC14B; OTTHUMP00000063774;
免疫原
- O60729 CC14B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRKSERRSSWAAAPPCSRRCSSTSPGVKKIRSSTQQDPRRRDPQDDVYLDITDRLCFAILYSRPKSASNVHYFSIDNELEYENFYADFGPLNLAMVYRYCCKINKKLKSITMLRKKIVHFTGSDQRKQANAAFLVGCYMVIYLGRTPEEAYRILIFGETSYIPFRDAAYGSCNFYITLLDCFHAVKKAMQYGFLNFNSFNLDEYEHYEKAENGDLNWIIPDRFIAFCGPHSRARLESGYHQHSPETYIQYFKNHNVTTIIRLNKRMYDAKRFTDAGFDHHDLFFADGSTPTDAIVKEFLDICENAEGAIAVHCKAGLGRTGTLIACYIMKHYRMTAAETIAWVRICRPGSVIGPQQQFLVMKQTNLWLEGDYFRQKLKGQENGQHRAAFSKLLSGVDDISINGVENQDQQEPEPYSDDDEINGVTQGDRLRALKSRRQSKTNAIPLTVILQSSVQSCKTSEPNISGSAGITKRTTRSASRKSSVKSLSISRTKTVLR
- A4D256 CC14C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRSSTLQDPRRRDPQDDVYVDITDRLRFAILYSRPKSASNVHYFSIDNELEYENFSEDFGPLNLAMVYRYCCKINKKLKSITMLRKKIVHFTGSDQRKQANAAFLVGCYMVIYLGRTPEAAYRILIFGDTPYIPFRDAAYGSCNFYITLLDCFHAVKKAMQYGFLNFNSFNLDEYEHYEKAENGDLNWIIPDRFIAFCGPHSRARLESGYHQHSPETYIQYFKNHNVTTIIRLNKRMYDAKRFTDAGFDHHDLFFADGSTPTDAIVKRFLDICENAEGAIAVHCKAGLGRTGTLIACYIMKHYRMTAAETIAWVRICRPGLVIGPQQQFLVMKQTSLWLEGDYFRQRLKGQENGQHRAAFSKLLSGVDDISINGVENQDQQEPKPYSDDDEINGVTQGDRSRALKRRRQSKTNDILLPSPLAVLTFTLCSVVIWWIVCDYILPILLF
PTMs - O60729/A4D256 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S9 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| T160 | Phosphorylation | Uniprot | |
| T426 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y19 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| Y386 | Phosphorylation | Uniprot | |
| T396 | Phosphorylation | Uniprot |
研究背景
Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.
Nucleus>Nucleolus. Nucleus>Nucleoplasm.
Note: Following DNA damage, translocates from the nucleolus to the nucleoplasm and interacts with FZR1/CDH1.
Interacts with FZR1/CDH1.
Composed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.
Dual-specificity phosphatase. Preferentially dephosphorylates proteins modified by proline-directed kinases (By similarity).
Endoplasmic reticulum membrane>Single-pass membrane protein.
Note: Retains its endoplasmic reticulum localization during mitosis.
Composed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
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