KAD6 Antibody - #AF0683

(2)  
製品: KAD6 Antibody
カタログ: AF0683
タンパク質の説明: Rabbit polyclonal antibody to KAD6
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit
分子量: 20kDa; 20kD(Calculated).
ユニプロット: Q9Y3D8
RRID: AB_2834355

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(90%), Bovine(90%), Horse(80%), Sheep(90%), Rabbit(80%)
クローナリティ:
Polyclonal
特異性:
KAD6 Antibody detects endogenous levels of total KAD6.
RRID:
AB_2834355
引用形式: Affinity Biosciences Cat# AF0683, RRID:AB_2834355.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AD-004; Adenylate kinase isoenzyme 6; Adrenal gland protein AD-004; AK/ATPase; AK6; CGI-137; CIP; Coilin-interacting nuclear ATPase protein; Dual activity adenylate kinase/ATPase; hCINAP; KAD6_HUMAN; TAF9;

免疫原

免疫原:
Uniprot:

Q9Y3D8(KAD6_HUMAN) >>Visit HPA database.

遺伝子(ID):
AK6(102157402)
発現特異性:
Q9Y3D8 KAD6_HUMAN:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, chorionic villi and the central nervous system.

タンパク質の説明:
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants encoding different isoforms.
タンパク質配列:
MLLPNILLTGTPGVGKTTLGKELASKSGLKYINVGDLAREEQLYDGYDEEYDCPILDEDRVVDELDNQMREGGVIVDYHGCDFFPERWFHIVFVLRTDTNVLYERLETRGYNEKKLTDNIQCEIFQVLYEEATASYKEEIVHQLPSNKPEELENNVDQILKWIEQWIKDHNS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
90
Bovine
90
Sheep
90
Horse
80
Rabbit
80
Chicken
70
Xenopus
60
Zebrafish
60
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y3D8 基板として

Site PTM Type Enzyme
K21 Ubiquitination
K26 Ubiquitination
K30 Ubiquitination
Y44 Phosphorylation
Y51 Phosphorylation
Y103 Phosphorylation
K115 Ubiquitination
K137 Ubiquitination
K148 Ubiquitination
K168 Ubiquitination
S172 Phosphorylation

研究背景

機能:

Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. AMP and dAMP are the preferred substrates, but CMP and dCMP are also good substrates. IMP is phosphorylated to a much lesser extent. All nucleoside triphosphates ATP, GTP, UTP, CTP, dATP, dCTP, dGTP, and TTP are accepted as phosphate donors. CTP is the best phosphate donor, followed by UTP, ATP, GTP and dCTP. May have a role in nuclear energy homeostasis. Has also ATPase activity. May be involved in regulation of Cajal body (CB) formation.

細胞の位置付け:

Nucleus>Nucleoplasm. Nucleus>Cajal body.
Note: Displays widespread diffuse nucleoplasmic distribution but not detected in nucleoli. Detected in Cajal bodies but not in all cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, chorionic villi and the central nervous system.

サブユニット構造:

Monomer and homodimer (By similarity). Interacts with COIL (via C-terminus).

タンパク質ファミリー:

Belongs to the adenylate kinase family. AK6 subfamily.

研究領域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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