Glycophorin A Antibody - #DF10170
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(3)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF10170, RRID:AB_2840749.
折りたたみ／展開
AI853584; Blood group--MN locus; CD_antigen=CD235a; CD235a; GLPA_HUMAN; Glycophorin A (MNS blood group); Glycophorin A; Glycophorin A, included; Glycophorin-A; GlycophorinA; GPA; GPErik; GpMiIII; GPSAT; GYPA; GYPA, included; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); MN; MN sialoglycoprotein; MNS; PAS-2; PAS2; Sialoglycoprotein alpha;
免疫原
- P02724 GLPA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYGKIIFVLLLSEIVSISASSTTGVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSVEIENPETSDQ
PTMs - P02724 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S21 | O-Glycosylation | Uniprot | |
| T22 | O-Glycosylation | Uniprot | |
| T23 | O-Glycosylation | Uniprot | |
| T29 | O-Glycosylation | Uniprot | |
| S30 | O-Glycosylation | Uniprot | |
| T31 | O-Glycosylation | Uniprot | |
| S32 | O-Glycosylation | Uniprot | |
| T36 | O-Glycosylation | Uniprot | |
| S38 | O-Glycosylation | Uniprot | |
| S41 | O-Glycosylation | Uniprot | |
| T44 | O-Glycosylation | Uniprot | |
| N45 | N-Glycosylation | Uniprot | |
| T52 | O-Glycosylation | Uniprot | |
| T56 | O-Glycosylation | Uniprot | |
| S63 | O-Glycosylation | Uniprot | |
| S66 | O-Glycosylation | Uniprot | |
| T69 | O-Glycosylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| S130 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S139 | Phosphorylation | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot |
研究背景
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).
The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).
Cell membrane>Single-pass type I membrane protein.
Note: Appears to be colocalized with SLC4A1.
Homodimer.
(Microbial infection) Interacts with Streptococcus gordonii hsa protein.
Belongs to the glycophorin A family.
研究領域
· Human Diseases > Infectious diseases: Parasitic > Malaria.
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
参考文献
Application: WB Species: mouse Sample: erythrocyte
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