CALCRL Antibody | Affinity Biosciences

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製品:	CALCRL Antibody
カタログ:	DF10203
タンパク質の説明:	Rabbit polyclonal antibody to CALCRL
アプリケーション:	WB
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	53 kDa; 53kD(Calculated).
ユニプロット:	Q16602
RRID:	AB_2840782

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(86%)

クローナリティ:

Polyclonal

特異性:

CALCRL Antibody detects endogenous levels of total CALCRL.

RRID:

AB_2840782
引用形式: Affinity Biosciences Cat# DF10203, RRID:AB_2840782.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

Calcitonin gene related peptide type 1 receptor; Calcitonin receptor like; Calcitonin receptor like receptor; CALCRL; CGRP type 1 receptor; CGRPR; CRLR;

免疫原

免疫原:

Uniprot:

Q16602(CALRL_HUMAN) >>Visit HPA database.

遺伝子(ID):

CALCRL(10203)

発現特異性:

Q16602 CALRL_HUMAN:

Predominantly expressed in the lung and heart.

タンパク質配列:

Q16602 CALRL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEKKCTLNFLVLLPFFMILVTAELEESPEDSIQLGVTRNKIMTAQYECYQKIMQDPIQQAEGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGNWFRHPASNRTWTNYTQCNVNTHEKVKTALNLFYLTIIGHGLSIASLLISLGIFFYFKSLSCQRITLHKNLFFSFVCNSVVTIIHLTAVANNQALVATNPVSCKVSQFIHLYLMGCNYFWMLCEGIYLHTLIVVAVFAEKQHLMWYYFLGWGFPLIPACIHAIARSLYYNDNCWISSDTHLLYIIHGPICAALLVNLFFLLNIVRVLITKLKVTHQAESNLYMKAVRATLILVPLLGIEFVLIPWRPEGKIAEEVYDYIMHILMHFQGLLVSTIFCFFNGEVQAILRRNWNQYKIQFGNSFSNSEALRSASYTVSTISDGPGYSHDCPSEHLNGKSIHDIENVLLKPENLYN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Dog

100

Pig

Horse

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16602 基板として

Q16602

Site	PTM Type	Source
S285	Phosphorylation	Uniprot
K403	Ubiquitination	Uniprot
S409	Phosphorylation	Uniprot
S445	Phosphorylation	Uniprot
Y460	Phosphorylation	Uniprot

研究背景

Q16602_CALRL_HUMAN

機能:

Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3 (By similarity). Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

組織特異性:

Predominantly expressed in the lung and heart.

サブユニット構造:

Heterodimer of CALCRL and RAMP3 (By similarity). Heterodimer of CALCRL and RAMP1 or CALCRL and RAMP2.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 2 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

参考文献

1). Intermedin in Paraventricular Nucleus Attenuates Ang II-Induced Sympathoexcitation through the Inhibition of NADPH Oxidase-Dependent ROS Generation in Obese Rats with Hypertension. International Journal of Molecular Sciences, 2019 (PubMed: 31466304) [IF=5.6]

Application: WB Species: rat Sample: paraventricular nucleus

Figure 1. |Plasma norepinephrine (NE) level (A. n = 6 or 7), systolic blood pressure (B. n = 6–7); and relative values of intermedin (IMD), calcitonin receptor–like receptor (CRLR) and receptor activity–modifying protein (RAMP) 1, RAMP2, and RAMP3 protein expression in the paraventricular nucleus (PVN) in control and obesity-related hypertensive (OH) rats (C–H. n = 3–4). Values are mean± SEM. * p < 0.05 versus control.

2). Exogenous CGRP Regulates Apoptosis and Autophagy to Alleviate Traumatic Brain Injury Through Akt/mTOR Signalling Pathway. NEUROCHEMICAL RESEARCH, 2020 (PubMed: 33063244) [IF=4.4]

Application: WB Species: mouse Sample: brain cortical

Fig. 1 CGRP alleviated the brain lesion induced by TBI. a Relative protein expression of CGRP in the brain cortical tissues with or without CGRP administration after TBI over time was detected by western blot (loading volume of CGRP: 15 μg).

3). Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes. ACTA DIABETOLOGICA, 2022 (PubMed: 34978596) [IF=3.8]

4). Correlation of vascular change with TRPV1, TRPV4, and TRPA1 in a rat model of inferior gluteal artery perforator flap. WOUND REPAIR AND REGENERATION, 2022 (PubMed: 35384152) [IF=2.9]

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CALCRL Antibody - #DF10203

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