CCR3 Antibody - #DF10205

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製品: CCR3 Antibody
カタログ: DF10205
タンパク質の説明: Rabbit polyclonal antibody to CCR3
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 41 kDa; 41kD(Calculated).
ユニプロット: P51677
RRID: AB_2840784

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(83%), Bovine(92%), Horse(83%), Sheep(83%), Dog(83%)
クローナリティ:
Polyclonal
特異性:
CCR3 Antibody detects endogenous levels of total CCR3.
RRID:
AB_2840784
引用形式: Affinity Biosciences Cat# DF10205, RRID:AB_2840784.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

B chemokine receptor; b-chemokine receptor; C C chemokine receptor type 3; C C CKR 3; C-C chemokine receptor type 3; C-C CKR-3; CC chemokine receptor 3; CC chemokine receptor type 3; CC CKR 3; CC R3; CC-CKR-3; CCCKR3; CCR 3; CCR-3; CCR3; CCR3_HUMAN; CD 193; CD193; CD193 antigen; Chemokine (C C motif) receptor 3; chemokine (C-C motif) receptor 3; Chemokine (CC) receptor 1 like 2; Chemokine CC motif receptor 3; Chemokine receptor 3; CK R3; CKR 3; CKR3; CMKB R3; CMKBR 3; CMKBR1L2; CMKBR3; Eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; Eotaxin receptor; Macrophage inflammatory protein 1 alpha receptor like 2; MGC102841; MIP1 Alpha RL2;

免疫原

免疫原:
Uniprot:

P51677(CCR3_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCR3(1232)
発現特異性:
P51677 CCR3_HUMAN:

In eosinophils as well as trace amounts in neutrophils and monocytes.

タンパク質配列:
MTTSLDTVETFGTTSYYDDVGLLCEKADTRALMAQFVPPLYSLVFTVGLLGNVVVVMILIKYRRLRIMTNIYLLNLAISDLLFLVTLPFWIHYVRGHNWVFGHGMCKLLSGFYHTGLYSEIFFIILLTIDRYLAIVHAVFALRARTVTFGVITSIVTWGLAVLAALPEFIFYETEELFEETLCSALYPEDTVYSWRHFHTLRMTIFCLVLPLLVMAICYTGIIKTLLRCPSKKKYKAIRLIFVIMAVFFIFWTPYNVAILLSSYQSILFGNDCERSKHLDLVMLVTEVIAYSHCCMNPVIYAFVGERFRKYLRHFFHRHLLMHLGRYIPFLPSEKLERTSSVSPSTAEPELSIVF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Pig
83
Horse
83
Sheep
83
Dog
83
Rabbit
67
Xenopus
50
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15. Subsequently transduces a signal by increasing the intracellular calcium ions level. In addition acts as a possible functional receptor for NARS1.

(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

In eosinophils as well as trace amounts in neutrophils and monocytes.

サブユニット構造:

(Microbial infection) Interacts with HIV-1 protein Tat.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

参考文献

1). SIRT1 regulates O-GlcNAcylation of tau through OGT. Aging-US, 2020 (PubMed: 32310828) [IF=5.2]

Application: WB    Species: human    Sample: HEK-293T cells

Figure 1. |SIRT1 decreases protein O-GlcNAcylation of tau in cells.(B) shSIRT1 plasmids or its control plasmids were transfected into HEK-293T cells. Recombinant human tau441 was immunoaffinity-purified with anti-GFP antibody from cells. Quantitative analysis of relative O-GlcNAcylation levels of tau after being normalized with total tau is shown as mean ± S.D. (n=3), ***, p < 0.001.
2). High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells. American Journal of Translational Research, 2019 (PubMed: 30788000) [IF=2.2]

Application: IHC    Species: human    Sample: primary NSCLC and bone metastasis tissues

Figure 2. |Expression of possible target chemokine receptors of CCL7 in primary NSCLC and bone metastasis tissues. A-C. IHC detection of CCR1, CCR2 and CCR3 expression in primary NSCLC and bone metastasis tissues. CCR1, CCR2 and CCR3 were mainly expressed on the cell membrane and in cytoplasm (arrow). D. qRT-PCR measurement of mRNA levels of CCR1, CCR2, CCR3 in primary NSCLC and bone metastasis tissues. *: P < 0.05.

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