DRD3 Antibody - #DF10212
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| 製品: | DRD3 Antibody |
| カタログ: | DF10212 |
| タンパク質の説明: | Rabbit polyclonal antibody to DRD3 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Horse, Sheep, Rabbit, Dog |
| 分子量: | 44 kDa; 44kD(Calculated). |
| ユニプロット: | P35462 |
| RRID: | AB_2815010 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF10212, RRID:AB_2815010.
折りたたみ／展開
3dopamine receptor; D; D(3) dopamine receptor; Dopamine D3 receptor; Dopamine receptor D3; DRD3; DRD3_HUMAN; ETM1; FET1;
免疫原
- P35462 DRD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASLSQLSSHLNYTCGAENSTGASQARPHAYYALSYCALILAIVFGNGLVCMAVLKERALQTTTNYLVVSLAVADLLVATLVMPWVVYLEVTGGVWNFSRICCDVFVTLDVMMCTASILNLCAISIDRYTAVVMPVHYQHGTGQSSCRRVALMITAVWVLAFAVSCPLLFGFNTTGDPTVCSISNPDFVIYSSVVSFYLPFGVTVLVYARIYVVLKQRRRKRILTRQNSQCNSVRPGFPQQTLSPDPAHLELKRYYSICQDTALGGPGFQERGGELKREEKTRNSLSPTIAPKLSLEVRKLSNGRLSTSLKLGPLQPRGVPLREKKATQMVAIVLGAFIVCWLPFFLTHVLNTHCQTCHVSPELYSATTWLGYVNSALNPVIYTTFNIEFRKAFLKILSC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P35462 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T142 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
| S233 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| T289 | Phosphorylation | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot |
研究背景
Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.
Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).
Palmitoylated.
Cell membrane>Multi-pass membrane protein.
Note: Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.
Brain.
Interacts with CLIC6 (By similarity). Interacts with GRK4. Interacts with PALM. Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA.
Belongs to the G-protein coupled receptor 1 family.
研究領域
· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.
· Organismal Systems > Nervous system > Dopaminergic synapse.
参考文献
Application: WB Species: Sample: BMDMs
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