P2RY1 Antibody - #DF10258

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製品: P2RY1 Antibody
カタログ: DF10258
タンパク質の説明: Rabbit polyclonal antibody to P2RY1
アプリケーション: WB IHC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 42 kDa; 42kD(Calculated).
ユニプロット: P47900
RRID: AB_2840836

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%), Xenopus(90%)
クローナリティ:
Polyclonal
特異性:
P2RY1 Antibody detects endogenous levels of total P2RY1.
RRID:
AB_2840836
引用形式: Affinity Biosciences Cat# DF10258, RRID:AB_2840836.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ATP receptor; P2 purinoceptor subtype Y1; P2RY1; P2RY1_HUMAN; P2Y purinoceptor 1; P2Y1; P2Y1 purinoceptor; Platelet ADP receptor; Purinergic receptor; Purinergic receptor P2Y G protein coupled 1; Purinergic receptor P2Y1;

免疫原

免疫原:

A synthesized peptide derived from human P2RY1, corresponding to a region within C-terminal amino acids.

Uniprot:

P47900(P2RY1_HUMAN) >>Visit HPA database.

遺伝子(ID):
P2RY1(5028)
タンパク質配列:
MTEVLWPAVPNGTDAAFLAGPGSSWGNSTVASTAAVSSSFKCALTKTGFQFYYLPAVYILVFIIGFLGNSVAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSILFLTCISAHRYSGVVYPLKSLGRLKKKNAICISVLVWLIVVVAISPILFYSGTGVRKNKTITCYDTTSDEYLRSYFIYSMCTTVAMFCVPLVLILGCYGLIVRALIYKDLDNSPLRRKSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSCVDPILYFLAGDTFRRRLSRATRKASRRSEANLQSKSEDMTLNILPEFKQNGDTSL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
90
Xenopus
90
Chicken
80
Zebrafish
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Receptor for extracellular adenine nucleotides such as ADP. In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and ultimately platelet aggregation.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Sensory system > Taste transduction.

参考文献

1). Activation of P2Y1R impedes intestinal mucosa repair during colitis. International journal of biological sciences, 2023 (PubMed: 37781034) [IF=8.2]

Application: WB    Species: Mouse    Sample:

Figure 3. P2Y1R expression was upregulated in the colonic epithelial cells of mice with DSS-induced colitis and in the colonic mucosa of UC patients. (A) Caco-2 monolayers were treated with ADP (1 mM) for 2 h and TNF-α (100 ng/mL) for 24 h, and then P2Y1R levels were measured by WB. (B) WT C57BL/6J mice were treated with or without 2% DSS for 7 days. Primary colonic epithelial cells were isolated from the mice, and the level of P2Y1R was determined by WB. (C) P2Y1R mRNA levels in inflamed and noninflamed colonic mucosa tissues of UC patients were obtained from GSE11223. (E) Representative P2Y1R immunohistochemical staining (× 200) and (D) quantitative analysis of P2Y1R expression (20 cases of healthy subjects, seven cases of inactive UC patients, and 34 cases of active UC patients). (F) Spearman correlation between P2Y1R expression and endoscopic score of colitis (r = 0.4769, p =.0016). Data represent the means ± SEM. *p < .05, **p < .01, ***p < .001.

Application: IHC    Species: Mouse    Sample:

Figure 3. P2Y1R expression was upregulated in the colonic epithelial cells of mice with DSS-induced colitis and in the colonic mucosa of UC patients. (A) Caco-2 monolayers were treated with ADP (1 mM) for 2 h and TNF-α (100 ng/mL) for 24 h, and then P2Y1R levels were measured by WB. (B) WT C57BL/6J mice were treated with or without 2% DSS for 7 days. Primary colonic epithelial cells were isolated from the mice, and the level of P2Y1R was determined by WB. (C) P2Y1R mRNA levels in inflamed and noninflamed colonic mucosa tissues of UC patients were obtained from GSE11223. (E) Representative P2Y1R immunohistochemical staining (× 200) and (D) quantitative analysis of P2Y1R expression (20 cases of healthy subjects, seven cases of inactive UC patients, and 34 cases of active UC patients). (F) Spearman correlation between P2Y1R expression and endoscopic score of colitis (r = 0.4769, p =.0016). Data represent the means ± SEM. *p < .05, **p < .01, ***p < .001.

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