Collagen XVII Antibody - #DF2913
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF2913, RRID:AB_2840902.
折りたたみ／展開
180 kDa bullous pemphigoid antigen 2; Alpha 1 type XVII collagen; BA16H23.2; BP 180; BP180; BPA 2; BPAG 2; BPAG2; Bullous pemphigoid antigen 2; COL17A1; Collagen 17; Collagen alpha 1 XVII chain; Collagen alpha 1(XVII) chain; Collagen alpha1 XVII chain; Collagen type XVII alpha 1; Collagen XVII alpha 1 polypeptide; CollagenXVII; Epidermolysis bullosa junctional localisata variant; FLJ60881; KIAA0204; LAD 1; LAD1;
免疫原
Detected in skin (PubMed:8618013). In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level) (PubMed:25676728). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
- Q9UMD9 COHA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASSQSVSGTYDATILDANLPSHVWSSTLPAGSSMGTYHNNMTTQSSSLLNTNAYSAGSVFGVPNNMASCSPTLHPGLSTSSSVFGMQNNLAPSLTTLSHGTTTTSTAYGVKKNMPQSPAAVNTGVSTSAACTTSVQSDDLLHKDCKFLILEKDNTPAKKEMELLIMTKDSGKVFTASPASIAATSFSEDTLKKEKQAAYNADSGLKAEANGDLKTVSTKGKTTTADIHSYGSSGGGGSGGGGGVGGAGGGPWGPAPAWCPCGSCCSWWKWLLGLLLTWLLLLGLLFGLIALAEEVRKLKARVDELERIRRSILPYGDSMDRIEKDRLQGMAPAAGADLDKIGLHSDSQEELWMFVRKKLMMEQENGNLRGSPGPKGDMGSPGPKGDRGFPGTPGIPGPLGHPGPQGPKGQKGSVGDPGMEGPMGQRGREGPMGPRGEAGPPGSGEKGERGAAGEPGPHGPPGVPGSVGPKGSSGSPGPQGPPGPVGLQGLRGEVGLPGVKGDKGPMGPPGPKGDQGEKGPRGLTGEPGMRGLPGAVGEPGAKGAMGPAGPDGHQGPRGEQGLTGMPGIRGPPGPSGDPGKPGLTGPQGPQGLPGTPGRPGIKGEPGAPGKIVTSEGSSMLTVPGPPGPPGAMGPPGPPGAPGPAGPAGLPGHQEVLNLQGPPGPPGPRGPPGPSIPGPPGPRGPPGEGLPGPPGPPGSFLSNSETFLSGPPGPPGPPGPKGDQGPPGPRGHQGEQGLPGFSTSGSSSFGLNLQGPPGPPGPQGPKGDKGDPGVPGALGIPSGPSEGGSSSTMYVSGPPGPPGPPGPPGSISSSGQEIQQYISEYMQSDSIRSYLSGVQGPPGPPGPPGPVTTITGETFDYSELASHVVSYLRTSGYGVSLFSSSISSEDILAVLQRDDVRQYLRQYLMGPRGPPGPPGASGDGSLLSLDYAELSSRILSYMSSSGISIGLPGPPGPPGLPGTSYEELLSLLRGSEFRGIVGPPGPPGPPGIPGNVWSSISVEDLSSYLHTAGLSFIPGPPGPPGPPGPRGPPGVSGALATYAAENSDSFRSELISYLTSPDVRSFIVGPPGPPGPQGPPGDSRLLSTDASHSRGSSSSSHSSSVRRGSSYSSSMSTGGGGAGSLGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGPPGQPGPQGPPGISKVFSAYSNVTADLMDFFQTYGAIQGPPGQKGEMGTPGPKGDRGPAGPPGHPGPPGPRGHKGEKGDKGDQVYAGRRRRRSIAVKP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UMD9 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y40 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| S70 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| Y80 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S93 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| T105 | Phosphorylation | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| T182 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| Y305 | Phosphorylation | Uniprot | |
| K365 | Acetylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K369 | Acetylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| Y396 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| Y512 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S544 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S640 | Phosphorylation | Uniprot | |
| S1124 | Phosphorylation | Uniprot | |
| S1131 | Phosphorylation | Uniprot | |
| T1284 | O-Glycosylation | Uniprot | |
| S1287 | Phosphorylation | Uniprot | |
| S1296 | Phosphorylation | Uniprot | |
| S1299 | Phosphorylation | Uniprot | |
| S1300 | Phosphorylation | Uniprot | |
| Y1395 | Phosphorylation | Uniprot | |
| N1421 | N-Glycosylation | Uniprot |
研究背景
May play a role in the integrity of hemidesmosome and the attachment of basal keratinocytes to the underlying basement membrane.
The 120 kDa linear IgA disease antigen is an anchoring filament component involved in dermal-epidermal cohesion. Is the target of linear IgA bullous dermatosis autoantibodies.
The intracellular/endo domain is disulfide-linked.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
The ectodomain is shedded from the surface of keratinocytes resulting in a 120-kDa soluble form, also named as 120 kDa linear IgA disease antigen. The shedding is mediated by membrane-bound metalloproteases. This cleavage is inhibited by phosphorylation at Ser-544.
Cell junction>Hemidesmosome. Membrane>Single-pass type II membrane protein.
Note: Localized along the plasma membrane of the hemidesmosome.
Secreted>Extracellular space>Extracellular matrix>Basement membrane.
Note: Exclusively localized to anchoring filaments. Localized to the epidermal side of split skin.
Secreted>Extracellular space>Extracellular matrix>Basement membrane.
Note: Localized in the lamina lucida beneath the hemidesmosomes.
Detected in skin. In the cornea, it is detected in the epithelial basement membrane, the epithelial cells, and at a lower level in stromal cells (at protein level). Stratified squamous epithelia. Found in hemidesmosomes. Expressed in cornea, oral mucosa, esophagus, intestine, kidney collecting ducts, ureter, bladder, urethra and thymus but is absent in lung, blood vessels, skeletal muscle and nerves.
Homotrimers of alpha 1(XVII)chains. Interacts (via cytoplasmic region) with ITGB4 (via cytoplasmic region). Interacts (via cytoplasmic region) with DST isoform 3 (via N-terminus). Interacts (via N-terminus) with PLEC. Interacts (via cytoplasmic region) with DSP.
研究領域
· Organismal Systems > Digestive system > Protein digestion and absorption.
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