Phospho-Cdk1/2 (Thr14) Antibody | Affinity Biosciences

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Figure 5 Sinomenine suppressed G2/M transition in HeyA8 cells by inhibiting the expression and the activity of CDK1.

製品:	Phospho-Cdk1/2 (Thr14) Antibody
カタログ:	DF2944
タンパク質の説明:	Rabbit polyclonal antibody to Phospho-Cdk1/2 (Thr14)
アプリケーション:	WB IHC
反応性:	Human, Mouse, Rat
予測:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量:	34kDa; 34kD(Calculated).
ユニプロット:	P06493 \| P24941
RRID:	AB_2840928

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

クローナリティ:

Polyclonal

特異性:

Phospho-Cdk1/2 (Thr14) Antibody detects endogenous levels of Cdk1/2 only when phosphorylated at Thr14.

RRID:

AB_2840928
引用形式: Affinity Biosciences Cat# DF2944, RRID:AB_2840928.

コンジュゲート:

Unconjugated.

精製:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

Cdc 2; Cdc2; CDC28A; CDK 1; CDK1; CDK1_HUMAN; CDKN1; CELL CYCLE CONTROLLER CDC2; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell division protein kinase 1; Cell Divsion Cycle 2 Protein; Cyclin Dependent Kinase 1; Cyclin-dependent kinase 1; DKFZp686L20222; MGC111195; p34 Cdk1; p34 protein kinase; P34CDC2; Cdc2 related protein kinase; cdc2-related protein kinase; CDC28; CDC2A; Cdk 2; CDK1; CDK2; CDK2_HUMAN; CDKN2; Cell devision kinase 2; Cell division protein kinase 2; Cyclin dependent kinase 2; cyclin dependent kinase 2-alpha; Cyclin-dependent kinase 2; kinase Cdc2; MPF; p33 protein kinase; p33(CDK2);

免疫原

免疫原:

Uniprot:

P06493(CDK1_HUMAN) >>Visit HPA database.

P24941(CDK2_HUMAN) >>Visit HPA database.

遺伝子(ID):

CDK2(1017) | CDK1(983)

発現特異性:

P06493 CDK1_HUMAN:

Isoform 2 is found in breast cancer tissues.

タンパク質配列:

P06493 CDK1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

P24941 CDK2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
Y4	Phosphorylation	P19525 (EIF2AK2)	Uniprot
T5	Phosphorylation		Uniprot
K6	Acetylation		Uniprot
K6	Sumoylation		Uniprot
K6	Ubiquitination		Uniprot
K9	Acetylation		Uniprot
K9	Sumoylation		Uniprot
K9	Ubiquitination		Uniprot
T14	Phosphorylation	Q99640 (PKMYT1)	Uniprot
Y15	Phosphorylation	P12931 (SRC) , P30291 (WEE1) , Q99640 (PKMYT1) , P04626 (ERBB2) , P07948 (LYN)	Uniprot
Y19	Phosphorylation		Uniprot
K20	Ubiquitination		Uniprot
K24	Sumoylation		Uniprot
K24	Ubiquitination		Uniprot
K33	Acetylation		Uniprot
K33	Sumoylation		Uniprot
K33	Ubiquitination		Uniprot
K34	Acetylation		Uniprot
K34	Sumoylation		Uniprot
K34	Ubiquitination		Uniprot
S39	Phosphorylation	P68400 (CSNK2A1)	Uniprot
S46	Phosphorylation		Uniprot
S53	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
Y77	Phosphorylation		Uniprot
K89	Ubiquitination		Uniprot
C119	S-Nitrosylation		Uniprot
S121	Phosphorylation		Uniprot
K130	Sumoylation		Uniprot
K130	Ubiquitination		Uniprot
K139	Sumoylation		Uniprot
K139	Ubiquitination		Uniprot
T141	Phosphorylation		Uniprot
K143	Ubiquitination		Uniprot
Y160	Phosphorylation		Uniprot
T161	Phosphorylation	P41279 (MAP3K8) , P50613 (CDK7)	Uniprot
S171	Phosphorylation		Uniprot
S178	Phosphorylation		Uniprot
K201	Sumoylation		Uniprot
K201	Ubiquitination		Uniprot
S208	Phosphorylation	O14757 (CHEK1)	Uniprot
T222	Phosphorylation		Uniprot
S233	Phosphorylation		Uniprot
K238	Sumoylation		Uniprot
K238	Ubiquitination		Uniprot
T240	Phosphorylation		Uniprot
K243	Ubiquitination		Uniprot
K245	Sumoylation		Uniprot
K245	Ubiquitination		Uniprot
S248	Phosphorylation		Uniprot
K254	Sumoylation		Uniprot
K254	Ubiquitination		Uniprot
S265	Phosphorylation		Uniprot
K266	Ubiquitination		Uniprot
Y270	Phosphorylation		Uniprot
K274	Ubiquitination		Uniprot
S277	Phosphorylation		Uniprot
K279	Sumoylation		Uniprot
K279	Ubiquitination		Uniprot
Y286	Phosphorylation		Uniprot
K295	Sumoylation		Uniprot
K295	Ubiquitination		Uniprot
K296	Ubiquitination		Uniprot

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
K6	Acetylation		Uniprot
K6	Ubiquitination		Uniprot
K9	Ubiquitination		Uniprot
T14	Phosphorylation	P30291 (WEE1)	Uniprot
Y15	Phosphorylation	P07948 (LYN) , P30291 (WEE1)	Uniprot
Y19	Phosphorylation		Uniprot
K20	Ubiquitination		Uniprot
K24	Ubiquitination		Uniprot
K33	Sumoylation		Uniprot
K33	Ubiquitination		Uniprot
T39	Phosphorylation	P31749 (AKT1)	Uniprot
S46	Phosphorylation	P50613 (CDK7)	Uniprot
T47	Phosphorylation		Uniprot
K56	Ubiquitination		Uniprot
K65	Ubiquitination		Uniprot
K129	Ubiquitination		Uniprot
T137	Phosphorylation		Uniprot
K142	Ubiquitination		Uniprot
T158	Phosphorylation		Uniprot
Y159	Phosphorylation		Uniprot
T160	Phosphorylation	P24941 (CDK2) , P28482 (MAPK1) , Q8IZL9 (CDK20) , P50613 (CDK7) , P27361 (MAPK3)	Uniprot
T165	Phosphorylation	P50613 (CDK7)	Uniprot
Y168	Phosphorylation	P50613 (CDK7)	Uniprot
K237	Ubiquitination		Uniprot
K250	Ubiquitination		Uniprot
K273	Ubiquitination		Uniprot
K278	Ubiquitination		Uniprot
K291	Ubiquitination		Uniprot
R297	Methylation		Uniprot

PTMs - P06493/P24941 酵素として

P06493
P24941

Substrate	Site	Source
A6NKT7 (RGPD3)	S1276	Uniprot
O00267 (SUPT5H)	S666	Uniprot
O00311 (CDC7)	T376	Uniprot
O00399 (DCTN6)	T186	Uniprot
O00418 (EEF2K)	S359	Uniprot
O00429 (DNM1L)	S616	Uniprot
O00443 (PIK3C2A)	S259	Uniprot
O00562 (PITPNM1)	T287	Uniprot
O00562 (PITPNM1)	S382	Uniprot
O00571 (DDX3X)	T204	Uniprot
O00571 (DDX3X)	T323	Uniprot
O14715 (RGPD8)	S1275	Uniprot
O14745 (SLC9A3R1)	S280	Uniprot
O14745 (SLC9A3R1)	S302	Uniprot
O14757 (CHEK1)	S286	Uniprot
O14757 (CHEK1)	S301	Uniprot
O14773 (TPP1)	T201	Uniprot
O14974 (PPP1R12A)	S432	Uniprot
O14974 (PPP1R12A)	S473	Uniprot
O14974 (PPP1R12A)	S601	Uniprot
O14980 (XPO1)	S391	Uniprot
O14994 (SYN3)	S470	Uniprot
O15151 (MDM4)	S96	Uniprot
O15287 (FANCG)	S383	Uniprot
O15287 (FANCG)	S387	Uniprot
O15287 (FANCG)	T487	Uniprot
O15350-1 (TP73)	T86	Uniprot
O15392 (BIRC5)	T34	Uniprot
O15446 (CD3EAP)	S285	Uniprot
O43521-2 (BCL2L11)	S44	Uniprot
O43663 (PRC1)	T481	Uniprot
O43683 (BUB1)	S593	Uniprot
O43683 (BUB1)	T609	Uniprot
O60331 (PIP5K1C)	S650	Uniprot
O60566 (BUB1B)	S543	Uniprot
O60566 (BUB1B)	T620	Uniprot
O60566 (BUB1B)	S670	Uniprot
O60566 (BUB1B)	S1043	Uniprot
O60610 (DIAPH1)	T768	Uniprot
O60701 (UGDH)	T461	Uniprot
O75122 (CLASP2)	S507	Uniprot
O75122 (CLASP2)	S541	Uniprot
O75152 (ZC3H11A)	T321	Uniprot
O75154 (RAB11FIP3)	S102	Uniprot
O94782 (USP1)	S313	Uniprot
O94916 (NFAT5)	T135	Uniprot
O95251 (KAT7)	T85	Uniprot
O95251 (KAT7)	T88	Uniprot
O95835-1 (LATS1)	T490	Uniprot
O95835 (LATS1)	S613	Uniprot
O95997 (PTTG1)	S165	Uniprot
P00338 (LDHA)	T248	Uniprot
P00519-1 (ABL1)	S569	Uniprot
P00519-2 (ABL1)	S588	Uniprot
P00533 (EGFR)	S1026	Uniprot
P02545-3 (LMNA)	T19	Uniprot
P02545-1 (LMNA)	S22	Uniprot
P02545 (LMNA)	S390	Uniprot
P02545 (LMNA)	S392	Uniprot
P04179 (SOD2)	S106	Uniprot
P04183 (TK1)	S13	Uniprot
P04637 (TP53)	S315	Uniprot
P05067 (APP)	T743	Uniprot
P05412 (JUN)	S63	Uniprot
P05412 (JUN)	S73	Uniprot
P05412 (JUN)	S243	Uniprot
P05783 (KRT18)	S34	Uniprot
P05787 (KRT8)	S432	Uniprot
P06400 (RB1)	S249	Uniprot
P06400 (RB1)	T252	Uniprot
P06400 (RB1)	T373	Uniprot
P06400 (RB1)	S807	Uniprot
P06400 (RB1)	S811	Uniprot
P06493-2 (CDK1)	Y19	Uniprot
P06748 (NPM1)	S70	Uniprot
P06748 (NPM1)	T199	Uniprot
P06748-2 (NPM1)	T205	Uniprot
P06748-2 (NPM1)	T208	Uniprot
P06748-1 (NPM1)	T219	Uniprot
P06748 (NPM1)	T234	Uniprot
P06748 (NPM1)	T237	Uniprot
P08047 (SP1)	T739	Uniprot
P08670 (VIM)	S55	Uniprot
P08670 (VIM)	S56	Uniprot
P0C1Z6 (TFPT)	S180	Uniprot
P10275 (AR)	S83	Uniprot
P10275 (AR)	S310	Uniprot
P10275 (AR)	S516	Uniprot
P10412 (HIST1H1E)	T18	Uniprot
P10415 (BCL2)	T56	Uniprot
P10415 (BCL2)	S70	Uniprot
P10636-6 (MAPT)	T95	Uniprot
P10636-8 (MAPT)	T153	Uniprot
P10636-8 (MAPT)	S202	Uniprot
P10636-8 (MAPT)	T205	Uniprot
P10636-8 (MAPT)	T212	Uniprot
P10636-8 (MAPT)	T231	Uniprot
P10636-8 (MAPT)	S235	Uniprot
P10636-8 (MAPT)	S404	Uniprot
P11171-2 (EPB41)	T60	Uniprot
P11171-2 (EPB41)	S679	Uniprot
P11387 (TOP1)	S112	Uniprot
P11387 (TOP1)	S394	Uniprot
P11388 (TOP2A)	S1213	Uniprot
P11388 (TOP2A)	S1247	Uniprot
P11388 (TOP2A)	S1354	Uniprot
P11388 (TOP2A)	S1361	Uniprot
P11388 (TOP2A)	S1393	Uniprot
P12270 (TPR)	S2073	Uniprot
P12272 (PTHLH)	T108	Uniprot
P12272 (PTHLH)	T121	Uniprot
P13051 (UNG)	S64	Uniprot
P14136-1 (GFAP)	S8	Uniprot
P14635 (CCNB1)	S126	Uniprot
P14635 (CCNB1)	S128	Uniprot
P15172 (MYOD1)	S5	Uniprot
P15172 (MYOD1)	S200	Uniprot
P15531 (NME1)	T94	Uniprot
P15531 (NME1)	S120	Uniprot
P15927 (RPA2)	S23	Uniprot
P15927 (RPA2)	S29	Uniprot
P15927 (RPA2)	S33	Uniprot
P16220 (CREB1)	S270	Uniprot
P16220 (CREB1)	S271	Uniprot
P16949 (STMN1)	S25	Uniprot
P16949-1 (STMN1)	S38	Uniprot
P17096 (HMGA1)	S36	Uniprot
P17096-2 (HMGA1)	T42	Uniprot
P17096 (HMGA1)	T53	Uniprot
P17096-2 (HMGA1)	T67	Uniprot
P17096 (HMGA1)	T78	Uniprot
P17302 (GJA1)	S255	Uniprot
P17661 (DES)	S32	Uniprot
P17676 (CEBPB)	S76	Uniprot
P17706-2 (PTPN2)	S304	Uniprot
P18031 (PTPN1)	S386	Uniprot
P18754-1 (RCC1)	S2	Uniprot
P18754 (RCC1)	S11	Uniprot
P18754 (RCC1)	T274	Uniprot
P18754 (RCC1)	S387	Uniprot
P18858 (LIG1)	S76	Uniprot
P19338 (NCL)	T121	Uniprot
P19338 (NCL)	T641	Uniprot
P19338 (NCL)	T707	Uniprot
P20338 (RAB4A)	S204	Uniprot
P20700 (LMNB1)	S23	Uniprot
P20700 (LMNB1)	S393	Uniprot
P21333 (FLNA)	S1084	Uniprot
P21333 (FLNA)	S1436	Uniprot
P21333 (FLNA)	S1459	Uniprot
P21333 (FLNA)	S1533	Uniprot
P21333 (FLNA)	S1630	Uniprot
P22234 (PAICS)	S27	Uniprot
P22314-1 (UBA1)	S4	Uniprot
P22314-1 (UBA1)	S835	Uniprot
P22392 (NME2)	T94	Uniprot
P23396 (RPS3)	T221	Uniprot
P23443 (RPS6KB1)	S394	Uniprot
P23443 (RPS6KB1)	S434	Uniprot
P23443 (RPS6KB1)	T444	Uniprot
P23443 (RPS6KB1)	S447	Uniprot
P23769 (GATA2)	T176	Uniprot
P24928 (POLR2A)	S1920	Uniprot
P24928 (POLR2A)	S1934	Uniprot
P25205 (MCM3)	S112	Uniprot
P26358 (DNMT1)	S154	Uniprot
P26368 (U2AF2)	S79	Uniprot
P27361-3 (MAPK3)	S343	Uniprot
P27816 (MAP4)	S696	Uniprot
P27816 (MAP4)	S787	Uniprot
P27816 (MAP4)	T892	Uniprot
P27816 (MAP4)	T901	Uniprot
P27816 (MAP4)	T917	Uniprot
P27824 (CANX)	S583	Uniprot
P29590 (PML)	S518	Uniprot
P29692 (EEF1D)	S133	Uniprot
P30101 (PDIA3)	S456	Uniprot
P30260 (CDC27)	T205	Uniprot
P30260 (CDC27)	T209	Uniprot
P30260 (CDC27)	S222	Uniprot
P30260 (CDC27)	T289	Uniprot
P30260 (CDC27)	S312	Uniprot
P30260 (CDC27)	T329	Uniprot
P30260 (CDC27)	T343	Uniprot
P30260 (CDC27)	S364	Uniprot
P30260 (CDC27)	S426	Uniprot
P30260 (CDC27)	T430	Uniprot
P30260 (CDC27)	S434	Uniprot
P30260 (CDC27)	S435	Uniprot
P30260 (CDC27)	T446	Uniprot
P30291 (WEE1)	S123	Uniprot
P30304 (CDC25A)	S18	Uniprot
P30304 (CDC25A)	S116	Uniprot
P30305-2 (CDC25B)	S146	Uniprot
P30305 (CDC25B)	S160	Uniprot
P30305-2 (CDC25B)	S307	Uniprot
P30305 (CDC25B)	S321	Uniprot
P30307 (CDC25C)	T48	Uniprot
P30307 (CDC25C)	T67	Uniprot
P30307-1 (CDC25C)	S122	Uniprot
P30307 (CDC25C)	T130	Uniprot
P30307 (CDC25C)	S168	Uniprot
P30307 (CDC25C)	S214	Uniprot
P31350-1 (RRM2)	S20	Uniprot
P31350 (RRM2)	T33	Uniprot
P31948 (STIP1)	T198	Uniprot
P31948 (STIP1)	T332	Uniprot
P33316-2 (DUT)	S11	Uniprot
P33316 (DUT)	S99	Uniprot
P33991 (MCM4)	T7	Uniprot
P33991 (MCM4)	T19	Uniprot
P33991 (MCM4)	S32	Uniprot
P33991 (MCM4)	S88	Uniprot
P33991 (MCM4)	T110	Uniprot
P33993 (MCM7)	S121	Uniprot
P35251 (RFC1)	T506	Uniprot
P35251 (RFC1)	S518	Uniprot
P35611 (ADD1)	S12	Uniprot
P35611 (ADD1)	S355	Uniprot
P36956 (SREBF1)	S439	Uniprot
P38398 (BRCA1)	S1189	Uniprot
P38398 (BRCA1)	S1191	Uniprot
P38398 (BRCA1)	S1497	Uniprot
P39748 (FEN1)	S187	Uniprot
P39880-1 (CUX1)	S1237	Uniprot
P39880 (CUX1)	S1270	Uniprot
P40763 (STAT3)	S727	Uniprot
P42166 (TMPO)	S424	Uniprot
P43307 (SSR1)	S268	Uniprot
P46013 (MKI67)	T761	Uniprot
P46013 (MKI67)	T1943	Uniprot
P46060 (RANGAP1)	T409	Uniprot
P46060 (RANGAP1)	S428	Uniprot
P46060 (RANGAP1)	S442	Uniprot
P46937 (YAP1)	T119	Uniprot
P46937 (YAP1)	S289	Uniprot
P46937 (YAP1)	S367	Uniprot
P47736-1 (RAP1GAP)	S484	Uniprot
P48200 (IREB2)	S157	Uniprot
P48681 (NES)	T315	Uniprot
P49418-1 (AMPH)	S272	Uniprot
P49418-1 (AMPH)	S276	Uniprot
P49418 (AMPH)	S285	Uniprot
P49450 (CENPA)	S68	Uniprot
P49459-2 (UBE2A)	S90	Uniprot
P49459 (UBE2A)	S120	Uniprot
P49715 (CEBPA)	S21	Uniprot
P49715 (CEBPA)	S234	Uniprot
P49792 (RANBP2)	T2153	Uniprot
P49792 (RANBP2)	S2246	Uniprot
P49792 (RANBP2)	S2251	Uniprot
P49792 (RANBP2)	S2276	Uniprot
P49792 (RANBP2)	S2280	Uniprot
P49915 (GMPS)	T318	Uniprot
P49916 (LIG3)	T191	Uniprot
P49916 (LIG3)	S210	Uniprot
P49916 (LIG3)	S913	Uniprot
P50570 (DNM2)	S764	Uniprot
P50570 (DNM2)	T766	Uniprot
P50613 (CDK7)	S164	Uniprot
P50613 (CDK7)	T170	Uniprot
P51587 (BRCA2)	S3291	Uniprot
P52732 (KIF11)	T926	Uniprot
P52926 (HMGA2)	S44	Uniprot
P52926 (HMGA2)	S59	Uniprot
P52948-2 (NUP98)	T529	Uniprot
P52948-2 (NUP98)	T536	Uniprot
P52948-2 (NUP98)	S595	Uniprot
P52948-2 (NUP98)	S606	Uniprot
P52948 (NUP98)	S612	Uniprot
P52948 (NUP98)	S623	Uniprot
P52948-2 (NUP98)	T653	Uniprot
P52948 (NUP98)	T670	Uniprot
P54132 (BLM)	S714	Uniprot
P54132 (BLM)	T766	Uniprot
P54274 (TERF1)	T344	Uniprot
P54274 (TERF1)	T371	Uniprot
P54578 (USP14)	T235	Uniprot
P55211 (CASP9)	T125	Uniprot
P61020-1 (RAB5B)	S123	Uniprot
P61978 (HNRNPK)	S216	Uniprot
P62136 (PPP1CA)	T320	Uniprot
P63279 (UBE2I)	S71	Uniprot
P67870 (CSNK2B)	S209	Uniprot
P68400-2 (CSNK2A1)	T208	Uniprot
P68400-2 (CSNK2A1)	T224	Uniprot
P68400-2 (CSNK2A1)	S226	Uniprot
P68400-2 (CSNK2A1)	S234	Uniprot
P68400 (CSNK2A1)	T344	Uniprot
P68400 (CSNK2A1)	T360	Uniprot
P68400 (CSNK2A1)	S362	Uniprot
P68400 (CSNK2A1)	S370	Uniprot
P82970 (HMGN5)	T31	Uniprot
Q01094 (E2F1)	S332	Uniprot
Q01094 (E2F1)	S337	Uniprot
Q01167 (FOXK2)	S373	Uniprot
Q01167 (FOXK2)	S428	Uniprot
Q01196 (RUNX1)	S21	Uniprot
Q01196 (RUNX1)	S249	Uniprot
Q01196 (RUNX1)	S266	Uniprot
Q01196 (RUNX1)	T273	Uniprot
Q01196-8 (RUNX1)	S276	Uniprot
Q01196-8 (RUNX1)	S293	Uniprot
Q01196-8 (RUNX1)	T300	Uniprot
Q01196-8 (RUNX1)	S303	Uniprot
Q01196 (RUNX1)	S397	Uniprot
Q01196-8 (RUNX1)	S424	Uniprot
Q01581 (HMGCS1)	S495	Uniprot
Q02539 (HIST1H1A)	T4	Uniprot
Q02750 (MAP2K1)	T286	Uniprot
Q02750 (MAP2K1)	T292	Uniprot
Q02880 (TOP2B)	S1424	Uniprot
Q02952 (AKAP12)	T767	Uniprot
Q03060-16 (CREM)	S87	Uniprot
Q03060-16 (CREM)	S110	Uniprot
Q03060-6 (CREM)	S136	Uniprot
Q04637 (EIF4G1)	S1231	Uniprot
Q05682-5 (CALD1)	T377	Uniprot
Q05682-4 (CALD1)	T383	Uniprot
Q05682-3 (CALD1)	T403	Uniprot
Q05682-6 (CALD1)	T409	Uniprot
Q05682-5 (CALD1)	S463	Uniprot
Q05682-4 (CALD1)	S469	Uniprot
Q05682-5 (CALD1)	T469	Uniprot
Q05682-4 (CALD1)	T475	Uniprot
Q05682-5 (CALD1)	S483	Uniprot
Q05682-3 (CALD1)	S489	Uniprot
Q05682-6 (CALD1)	S494	Uniprot
Q05682-3 (CALD1)	T495	Uniprot
Q05682-5 (CALD1)	S498	Uniprot
Q05682-6 (CALD1)	T500	Uniprot
Q05682-4 (CALD1)	S504	Uniprot
Q05682-3 (CALD1)	S509	Uniprot
Q05682-6 (CALD1)	S514	Uniprot
Q05682-3 (CALD1)	S524	Uniprot
Q05682-6 (CALD1)	S529	Uniprot
Q05682-1 (CALD1)	T638	Uniprot
Q05682-1 (CALD1)	S724	Uniprot
Q05682-1 (CALD1)	T730	Uniprot
Q05682-1 (CALD1)	S744	Uniprot
Q05682-1 (CALD1)	S759	Uniprot
Q06413 (MEF2C)	S396	Uniprot
Q06481 (APLP2)	T736	Uniprot
Q06830 (PRDX1)	T90	Uniprot
Q07666 (KHDRBS1)	T317	Uniprot
Q07820 (MCL1)	S64	Uniprot
Q07820 (MCL1)	T92	Uniprot
Q08050 (FOXM1)	S251	Uniprot
Q08050-2 (FOXM1)	T596	Uniprot
Q08050 (FOXM1)	T600	Uniprot
Q08050-1 (FOXM1)	T611	Uniprot
Q08050 (FOXM1)	T620	Uniprot
Q08050-3 (FOXM1)	T649	Uniprot
Q08379 (GOLGA2)	S37	Uniprot
Q09472 (EP300)	S1038	Uniprot
Q09472 (EP300)	S2039	Uniprot
Q09666 (AHNAK)	S5763	Uniprot
Q12778 (FOXO1)	S249	Uniprot
Q12888 (TP53BP1)	T1609	Uniprot
Q12888 (TP53BP1)	S1678	Uniprot
Q12959 (DLG1)	S158	Uniprot
Q12959 (DLG1)	S443	Uniprot
Q13042 (CDC16)	S112	Uniprot
Q13042 (CDC16)	S560	Uniprot
Q13042 (CDC16)	T581	Uniprot
Q13188 (STK3)	S385	Uniprot
Q13415 (ORC1)	S258	Uniprot
Q13415 (ORC1)	S273	Uniprot
Q13415 (ORC1)	T375	Uniprot
Q13428 (TCOF1)	S156	Uniprot
Q13428 (TCOF1)	S583	Uniprot
Q13428 (TCOF1)	T983	Uniprot
Q13501 (SQSTM1)	T269	Uniprot
Q13501 (SQSTM1)	S272	Uniprot
Q13535 (ATR)	S428	Uniprot
Q13541 (EIF4EBP1)	T37	Uniprot
Q13541 (EIF4EBP1)	T46	Uniprot
Q13541 (EIF4EBP1)	S65	Uniprot
Q13541 (EIF4EBP1)	T70	Uniprot
Q13541 (EIF4EBP1)	S83	Uniprot
Q13586 (STIM1)	S668	Uniprot
Q13950 (RUNX2)	S465	Uniprot
Q14005-3 (IL16)	T56	Uniprot
Q14005-1 (IL16)	T757	Uniprot
Q14157 (UBAP2L)	S454	Uniprot
Q14202 (ZMYM3)	T826	Uniprot
Q14203 (DCTN1)	S212	Uniprot
Q14244 (MAP7)	S209	Uniprot
Q14247 (CTTN)	S405	Uniprot
Q14493 (SLBP)	T62	Uniprot
Q14643 (ITPR1)	S421	Uniprot
Q14643 (ITPR1)	T800	Uniprot
Q14674 (ESPL1)	S1126	Uniprot
Q14739 (LBR)	S71	Uniprot
Q14739 (LBR)	S86	Uniprot
Q14790 (CASP8)	S387	Uniprot
Q14807 (KIF22)	S427	Uniprot
Q14807 (KIF22)	T463	Uniprot
Q14978 (NOLC1)	T607	Uniprot
Q14978 (NOLC1)	T610	Uniprot
Q14980 (NUMA1)	T2015	Uniprot
Q14980 (NUMA1)	T2055	Uniprot
Q14980 (NUMA1)	S2087	Uniprot
Q14980 (NUMA1)	T2106	Uniprot
Q14C86 (GAPVD1)	S950	Uniprot
Q14CS0 (UBXN2B)	S56	Uniprot
Q14CS0 (UBXN2B)	T59	Uniprot
Q15149-7 (PLEC)	T4370	Uniprot
Q15149-8 (PLEC)	T4380	Uniprot
Q15149-9 (PLEC)	T4388	Uniprot
Q15149-5 (PLEC)	T4402	Uniprot
Q15149-6 (PLEC)	T4406	Uniprot
Q15149 (PLEC)	T4539	Uniprot
Q15398 (DLGAP5)	T329	Uniprot
Q15398 (DLGAP5)	S618	Uniprot
Q15398 (DLGAP5)	T639	Uniprot
Q15398 (DLGAP5)	S839	Uniprot
Q15717 (ELAVL1)	S202	Uniprot
Q15910 (EZH2)	T345	Uniprot
Q15910 (EZH2)	T487	Uniprot
Q16513 (PKN2)	S535	Uniprot
Q16659 (MAPK6)	S684	Uniprot
Q16659 (MAPK6)	S688	Uniprot
Q16659 (MAPK6)	T698	Uniprot
Q16659 (MAPK6)	S705	Uniprot
Q16665 (HIF1A)	S668	Uniprot
Q2M2I8 (AAK1)	T389	Uniprot
Q2NKX8 (ERCC6L)	T1063	Uniprot
Q38SD2 (LRRK1)	T1427	Uniprot
Q53EZ4 (CEP55)	S425	Uniprot
Q53EZ4 (CEP55)	S428	Uniprot
Q5BJF6-3 (ODF2)	S796	Uniprot
Q5FBB7 (SGO1)	T346	Uniprot
Q6PGQ7 (BORA)	S252	Uniprot
Q6Y7W6-3 (GIGYF2)	S30	Uniprot
Q7Z3J3 (RGPD4)	S1276	Uniprot
Q86WB0 (ZC3HC1)	S395	Uniprot
Q8IWB6 (TEX14)	T618	Uniprot
Q8IWB6 (TEX14)	T727	Uniprot
Q8IWB6 (TEX14)	T728	Uniprot
Q8IX03 (WWC1)	S542	Uniprot
Q8IX03 (WWC1)	S931	Uniprot
Q8IXJ6 (SIRT2)	S368	Uniprot
Q8IZD2 (KMT2E)	T912	Uniprot
Q8IZP0 (ABI1)	S216	Uniprot
Q8N122 (RPTOR)	S696	Uniprot
Q8N122 (RPTOR)	T706	Uniprot
Q8NEB9 (PIK3C3)	T159	Uniprot
Q8NFH8-2 (REPS2)	S411	Uniprot
Q8NFH8 (REPS2)	S463	Uniprot
Q8NHV4 (NEDD1)	T550	Uniprot
Q8TAP9 (MPLKIP)	S93	Uniprot
Q8TAP9 (MPLKIP)	S104	Uniprot
Q8TAP9 (MPLKIP)	T120	Uniprot
Q8TEM1-1 (NUP210)	S1881	Uniprot
Q8TF76 (HASPIN)	T128	Uniprot
Q8WWK9 (CKAP2)	T623	Uniprot
Q8WX93 (PALLD)	S641	Uniprot
Q8WX93 (PALLD)	S766	Uniprot
Q92574 (TSC1)	T417	Uniprot
Q92574-1 (TSC1)	S584	Uniprot
Q92574 (TSC1)	T1047	Uniprot
Q92934 (BAD)	S91	Uniprot
Q92993 (KAT5)	S86	Uniprot
Q92993 (KAT5)	S90	Uniprot
Q92993-3 (KAT5)	S119	Uniprot
Q92993-3 (KAT5)	S123	Uniprot
Q93045-1 (STMN2)	S73	Uniprot
Q96C19 (EFHD2)	S74	Uniprot
Q96C36 (PYCR2)	S304	Uniprot
Q96EB6 (SIRT1)	T530	Uniprot
Q96EB6 (SIRT1)	S540	Uniprot
Q96FF9 (CDCA5)	S21	Uniprot
Q96FF9 (CDCA5)	S33	Uniprot
Q96FF9 (CDCA5)	T48	Uniprot
Q96FF9 (CDCA5)	S75	Uniprot
Q96FF9 (CDCA5)	S79	Uniprot
Q96FF9 (CDCA5)	S83	Uniprot
Q96FF9 (CDCA5)	T111	Uniprot
Q96FF9 (CDCA5)	T115	Uniprot
Q96FF9 (CDCA5)	S126	Uniprot
Q96FF9 (CDCA5)	S139	Uniprot
Q96FF9 (CDCA5)	T151	Uniprot
Q96FF9 (CDCA5)	T159	Uniprot
Q96FF9 (CDCA5)	S164	Uniprot
Q96FF9 (CDCA5)	S181	Uniprot
Q96FF9 (CDCA5)	S209	Uniprot
Q96JH7 (VCPIP1)	T761	Uniprot
Q96JH7 (VCPIP1)	S768	Uniprot
Q96KB5 (PBK)	T9	Uniprot
Q96Q89-3 (KIF20B)	T1604	Uniprot
Q96Q89 (KIF20B)	T1644	Uniprot
Q96R06 (SPAG5)	S135	Uniprot
Q96R06 (SPAG5)	S249	Uniprot
Q96RL1 (UIMC1)	S677	Uniprot
Q96T23 (RSF1)	S1375	Uniprot
Q96T88 (UHRF1)	S639	Uniprot
Q99638 (RAD9A)	S277	Uniprot
Q99638 (RAD9A)	T292	Uniprot
Q99638 (RAD9A)	S328	Uniprot
Q99638 (RAD9A)	S336	Uniprot
Q99638 (RAD9A)	T355	Uniprot
Q99661 (KIF2C)	T537	Uniprot
Q99666 (RGPD6)	S1275	Uniprot
Q99728 (BARD1)	S148	Uniprot
Q99728 (BARD1)	S251	Uniprot
Q99728 (BARD1)	S288	Uniprot
Q99728 (BARD1)	T299	Uniprot
Q9BPX3 (NCAPG)	T308	Uniprot
Q9BPX3 (NCAPG)	T332	Uniprot
Q9BPX3 (NCAPG)	T931	Uniprot
Q9BXS6 (NUSAP1)	T300	Uniprot
Q9BXS6 (NUSAP1)	T338	Uniprot
Q9BYG3 (NIFK)	T238	Uniprot
Q9GZM8 (NDEL1)	T219	Uniprot
Q9GZM8 (NDEL1)	S242	Uniprot
Q9GZM8 (NDEL1)	T245	Uniprot
Q9GZV5 (WWTR1)	S90	Uniprot
Q9GZV5 (WWTR1)	S105	Uniprot
Q9GZV5 (WWTR1)	T285	Uniprot
Q9GZV5 (WWTR1)	T326	Uniprot
Q9GZV5 (WWTR1)	T346	Uniprot
Q9H0H5 (RACGAP1)	T588	Uniprot
Q9H1A4 (ANAPC1)	T291	Uniprot
Q9H1A4 (ANAPC1)	S355	Uniprot
Q9H1A4 (ANAPC1)	S373	Uniprot
Q9H1A4 (ANAPC1)	S377	Uniprot
Q9H1A4 (ANAPC1)	T530	Uniprot
Q9H1A4 (ANAPC1)	T537	Uniprot
Q9H1A4 (ANAPC1)	S547	Uniprot
Q9H1A4 (ANAPC1)	S555	Uniprot
Q9H1A4 (ANAPC1)	S688	Uniprot
Q9H1A4 (ANAPC1)	T701	Uniprot
Q9H1E3 (NUCKS1)	S181	Uniprot
Q9H467 (CUEDC2)	S110	Uniprot
Q9H4X1-1 (RGCC)	T111	Uniprot
Q9H8V3 (ECT2)	T373	Uniprot
Q9H8V3 (ECT2)	T444	Uniprot
Q9H8V3-4 (ECT2)	T815	Uniprot
Q9H8V3 (ECT2)	T846	Uniprot
Q9H9S0 (NANOG)	S71	Uniprot
Q9HCH0 (NCKAP5L)	S436	Uniprot
Q9HCH0 (NCKAP5L)	S447	Uniprot
Q9HCH0 (NCKAP5L)	S466	Uniprot
Q9HCH0 (NCKAP5L)	S473	Uniprot
Q9HCH0 (NCKAP5L)	S567	Uniprot
Q9HCH0 (NCKAP5L)	S573	Uniprot
Q9HCH0 (NCKAP5L)	S763	Uniprot
Q9NQR1 (KMT5A)	S100	Uniprot
Q9NQS7 (INCENP)	T59	Uniprot
Q9NQS7 (INCENP)	T412	Uniprot
Q9NQX3 (GPHN)	S270	Uniprot
Q9NTG7 (SIRT3)	T150	Uniprot
Q9NTG7 (SIRT3)	S159	Uniprot
Q9NTI5 (PDS5B)	T1370	Uniprot
Q9NXR1-2 (NDE1)	T215	Uniprot
Q9NXR1-2 (NDE1)	S239	Uniprot
Q9NXR1 (NDE1)	T246	Uniprot
Q9NZH5 (PTTG2)	S165	Uniprot
Q9NZJ0 (DTL)	T464	Uniprot
Q9NZT2 (OGFR)	S378	Uniprot
Q9UBF8 (PI4KB)	S266	Uniprot
Q9UHD8-2 (SEPT9)	T24	Uniprot
Q9UJ70 (NAGK)	S76	Uniprot
Q9UJX2 (CDC23)	T562	Uniprot
Q9UJX2 (CDC23)	T565	Uniprot
Q9UJX5 (ANAPC4)	S779	Uniprot
Q9UK53-2 (ING1)	S126	Uniprot
Q9UKX7 (NUP50)	S221	Uniprot
Q9ULC4 (MCTS1)	S118	Uniprot
Q9ULW0 (TPX2)	T72	Uniprot
Q9ULW0 (TPX2)	S738	Uniprot
Q9UNZ2-1 (NSFL1C)	S140	Uniprot
Q9UPP1 (PHF8)	S69	Uniprot
Q9UPP1 (PHF8)	S120	Uniprot
Q9UQ35 (SRRM2)	T866	Uniprot
Q9UQ35 (SRRM2)	T1413	Uniprot
Q9UQR0 (SCML2)	T305	Uniprot
Q9UQR0 (SCML2)	S511	Uniprot
Q9UQR0 (SCML2)	S590	Uniprot
Q9Y2I6 (NINL)	S185	Uniprot
Q9Y2I6 (NINL)	S589	Uniprot
Q9Y2V2 (CARHSP1)	T23	Uniprot
Q9Y3Z3 (SAMHD1)	T592	Uniprot
Q9Y4H2 (IRS2)	S391	Uniprot
Q9Y5N6 (ORC6)	T195	Uniprot
Q9Y5T5 (USP16)	S552	Uniprot
Q9Y6I3-3 (EPN1)	S357	Uniprot
Q9Y6I3 (EPN1)	S382	Uniprot
Q9Y6Q9 (NCOA3)	S728	Uniprot
Q9Y6Q9 (NCOA3)	S867	Uniprot

Substrate	Site	Source
A1KXE4 (FAM168B)	T57	Uniprot
O00267 (SUPT5H)	S666	Uniprot
O00267 (SUPT5H)	T775	Uniprot
O00267 (SUPT5H)	T791	Uniprot
O00311 (CDC7)	T376	Uniprot
O00429 (DNM1L)	S616	Uniprot
O00571 (DDX3X)	T204	Uniprot
O14497 (ARID1A)	S363	Uniprot
O14497 (ARID1A)	S1320	Uniprot
O14497 (ARID1A)	S1322	Uniprot
O14686 (KMT2D)	T5374	Uniprot
O14757 (CHEK1)	S286	Uniprot
O14757 (CHEK1)	S301	Uniprot
O15014 (ZNF609)	T722	Uniprot
O15350-1 (TP73)	T86	Uniprot
O43395 (PRPF3)	T167	Uniprot
O43463 (SUV39H1)	S391	Uniprot
O43663-1 (PRC1)	T470	Uniprot
O43663-1 (PRC1)	T481	Uniprot
O60271 (SPAG9)	T586	Uniprot
O60504 (SORBS3)	T562	Uniprot
O60504 (SORBS3)	S563	Uniprot
O60934 (NBN)	S432	Uniprot
O75152 (ZC3H11A)	T321	Uniprot
O75179 (ANKRD17)	S2042	Uniprot
O75179 (ANKRD17)	S2044	Uniprot
O75179 (ANKRD17)	S2045	Uniprot
O75179 (ANKRD17)	S2401	Uniprot
O75469 (NR1I2)	S114	Uniprot
O75469 (NR1I2)	T133	Uniprot
O75469 (NR1I2)	T135	Uniprot
O75469 (NR1I2)	S167	Uniprot
O75469 (NR1I2)	S200	Uniprot
O75469 (NR1I2)	S350	Uniprot
O75533 (SF3B1)	T142	Uniprot
O75533 (SF3B1)	T211	Uniprot
O75533 (SF3B1)	T244	Uniprot
O75533 (SF3B1)	T248	Uniprot
O75533 (SF3B1)	S259	Uniprot
O75533 (SF3B1)	T261	Uniprot
O75533 (SF3B1)	T267	Uniprot
O75533 (SF3B1)	T313	Uniprot
O75533 (SF3B1)	T354	Uniprot
O75533 (SF3B1)	T426	Uniprot
O75533 (SF3B1)	T432	Uniprot
O75533 (SF3B1)	T434	Uniprot
O75533 (SF3B1)	T436	Uniprot
O75553 (DAB1)	S524	Uniprot
O75616 (ERAL1)	S173	Uniprot
O94776 (MTA2)	S435	Uniprot
O94776 (MTA2)	T438	Uniprot
O94776 (MTA2)	T439	Uniprot
O94776 (MTA2)	S440	Uniprot
O95251 (KAT7)	T88	Uniprot
O95295 (SNAPIN)	S133	Uniprot
P03372 (ESR1)	S104	Uniprot
P03372 (ESR1)	S106	Uniprot
P03372 (ESR1)	S118	Uniprot
P03372 (ESR1)	S294	Uniprot
P04183 (TK1)	S13	Uniprot
P04637 (TP53)	S313	Uniprot
P04637 (TP53)	S314	Uniprot
P04637 (TP53)	S315	Uniprot
P04637-1 (TP53)	S392	Uniprot
P06400 (RB1)	S230	Uniprot
P06400 (RB1)	S249	Uniprot
P06400 (RB1)	T252	Uniprot
P06400 (RB1)	T356	Uniprot
P06400 (RB1)	T373	Uniprot
P06400 (RB1)	S567	Uniprot
P06400 (RB1)	S608	Uniprot
P06400 (RB1)	S612	Uniprot
P06400 (RB1)	S807	Uniprot
P06400 (RB1)	S811	Uniprot
P06400 (RB1)	T821	Uniprot
P06400 (RB1)	T826	Uniprot
P06401 (PGR)	S20	Uniprot
P06401 (PGR)	S25	Uniprot
P06401 (PGR)	S162	Uniprot
P06401 (PGR)	S190	Uniprot
P06401 (PGR)	S213	Uniprot
P06401 (PGR)	S294	Uniprot
P06401 (PGR)	S400	Uniprot
P06401 (PGR)	T430	Uniprot
P06401 (PGR)	S554	Uniprot
P06401 (PGR)	S676	Uniprot
P06748 (NPM1)	S70	Uniprot
P06748 (NPM1)	T199	Uniprot
P06748 (NPM1)	T234	Uniprot
P08621 (SNRNP70)	S226	Uniprot
P08670 (VIM)	S55	Uniprot
P08670 (VIM)	S56	Uniprot
P10244-1 (MYBL2)	T266	Uniprot
P10244-1 (MYBL2)	S393	Uniprot
P10244-1 (MYBL2)	T405	Uniprot
P10244-1 (MYBL2)	T440	Uniprot
P10244-1 (MYBL2)	T444	Uniprot
P10244-1 (MYBL2)	S452	Uniprot
P10244-1 (MYBL2)	T487	Uniprot
P10244-1 (MYBL2)	T494	Uniprot
P10244-1 (MYBL2)	T515	Uniprot
P10244-1 (MYBL2)	T518	Uniprot
P10244 (MYBL2)	T520	Uniprot
P10244-1 (MYBL2)	S577	Uniprot
P10412 (HIST1H1E)	T18	Uniprot
P10644 (PRKAR1A)	S83	Uniprot
P10644-1 (PRKAR1A)	T209	Uniprot
P12268 (IMPDH2)	S122	Uniprot
P12270 (TPR)	S1676	Uniprot
P12270 (TPR)	T1677	Uniprot
P12270 (TPR)	S1679	Uniprot
P12272-1 (PTHLH)	T108	Uniprot
P12272 (PTHLH)	T121	Uniprot
P12956 (XRCC6)	T455	Uniprot
P13051 (UNG)	S12	Uniprot
P13639 (EEF2)	T435	Uniprot
P13639 (EEF2)	S595	Uniprot
P14859 (POU2F1)	S269	Uniprot
P14859 (POU2F1)	T270	Uniprot
P15172 (MYOD1)	S200	Uniprot
P15336 (ATF2)	T116	Uniprot
P15923 (TCF3)	S139	Uniprot
P15923 (TCF3)	S245	Uniprot
P15927 (RPA2)	S23	Uniprot
P15927 (RPA2)	S29	Uniprot
P15927 (RPA2)	S33	Uniprot
P16949 (STMN1)	S25	Uniprot
P16949 (STMN1)	S38	Uniprot
P17096 (HMGA1)	S36	Uniprot
P17480 (UBTF)	S389	Uniprot
P17480 (UBTF)	S484	Uniprot
P17676 (CEBPB)	T235	Uniprot
P17706 (PTPN2)	S304	Uniprot
P18858 (LIG1)	S51	Uniprot
P18858 (LIG1)	S76	Uniprot
P18858 (LIG1)	S91	Uniprot
P18858 (LIG1)	T195	Uniprot
P18858 (LIG1)	T197	Uniprot
P18858 (LIG1)	T233	Uniprot
P18887 (XRCC1)	S226	Uniprot
P18887 (XRCC1)	S229	Uniprot
P19338 (NCL)	S67	Uniprot
P19338 (NCL)	T76	Uniprot
P19338 (NCL)	T121	Uniprot
P20248 (CCNA2)	S154	Uniprot
P22234 (PAICS)	S27	Uniprot
P22674 (CCNO)	S81	Uniprot
P23258 (TUBG1)	S80	Uniprot
P23396 (RPS3)	T220	Uniprot
P23396 (RPS3)	T221	Uniprot
P23511-2 (NFYA)	S291	Uniprot
P23511-2 (NFYA)	S297	Uniprot
P23511-1 (NFYA)	S320	Uniprot
P23511-1 (NFYA)	S326	Uniprot
P23771 (GATA3)	T156	Uniprot
P24864 (CCNE1)	S387	Uniprot
P24864 (CCNE1)	T395	Uniprot
P24864 (CCNE1)	S399	Uniprot
P24928 (POLR2A)	S1882	Uniprot
P24928 (POLR2A)	T1884	Uniprot
P24928 (POLR2A)	T1885	Uniprot
P24928 (POLR2A)	T1915	Uniprot
P24928 (POLR2A)	S1917	Uniprot
P24941-1 (CDK2)	Y19	Uniprot
P24941 (CDK2)	T160	Uniprot
P25054 (APC)	S1360	Uniprot
P25098 (GRK2)	S670	Uniprot
P25205 (MCM3)	T722	Uniprot
P26358 (DNMT1)	S154	Uniprot
P28324 (ELK4)	T194	Uniprot
P28324 (ELK4)	S387	Uniprot
P28749 (RBL1)	S640	Uniprot
P28749 (RBL1)	S650	Uniprot
P29350 (PTPN6)	S591	Uniprot
P29374 (ARID4A)	S864	Uniprot
P29374 (ARID4A)	S1007	Uniprot
P29374 (ARID4A)	T1124	Uniprot
P29374 (ARID4A)	S1140	Uniprot
P29374 (ARID4A)	S1145	Uniprot
P29590 (PML)	S518	Uniprot
P29966 (MARCKS)	S26	Uniprot
P29966 (MARCKS)	S27	Uniprot
P29966 (MARCKS)	S118	Uniprot
P29966 (MARCKS)	S131	Uniprot
P29966 (MARCKS)	S132	Uniprot
P29966 (MARCKS)	T133	Uniprot
P29966 (MARCKS)	S134	Uniprot
P29966 (MARCKS)	S135	Uniprot
P29966 (MARCKS)	S147	Uniprot
P29966 (MARCKS)	T150	Uniprot
P30050 (RPL12)	S38	Uniprot
P30291 (WEE1)	S123	Uniprot
P30304 (CDC25A)	S263	Uniprot
P30307 (CDC25C)	S214	Uniprot
P31350 (RRM2)	S20	Uniprot
P31350 (RRM2)	T33	Uniprot
P31943 (HNRNPH1)	S104	Uniprot
P32322 (PYCR1)	S301	Uniprot
P32322 (PYCR1)	S303	Uniprot
P33981-1 (TTK)	T468	Uniprot
P33991 (MCM4)	S3	Uniprot
P33991 (MCM4)	T7	Uniprot
P33991 (MCM4)	T19	Uniprot
P33991 (MCM4)	S32	Uniprot
P33991 (MCM4)	T53	Uniprot
P33991 (MCM4)	S54	Uniprot
P33991 (MCM4)	T110	Uniprot
P33993 (MCM7)	S121	Uniprot
P33993 (MCM7)	S365	Uniprot
P35269 (GTF2F1)	T445	Uniprot
P35269 (GTF2F1)	T446	Uniprot
P35611 (ADD1)	S613	Uniprot
P35611 (ADD1)	T614	Uniprot
P38398-3 (BRCA1)	S393	Uniprot
P38398-8 (BRCA1)	S1450	Uniprot
P38398 (BRCA1)	S1497	Uniprot
P38432 (COIL)	S184	Uniprot
P38919 (EIF4A3)	T163	Uniprot
P38936 (CDKN1A)	S130	Uniprot
P42677 (RPS27)	S27	Uniprot
P46013 (MKI67)	S357	Uniprot
P46527 (CDKN1B)	T187	Uniprot
P48200 (IREB2)	S157	Uniprot
P48634-2 (PRRC2A)	T1340	Uniprot
P49454 (CENPF)	T154	Uniprot
P49454 (CENPF)	S156	Uniprot
P49459-2 (UBE2A)	S90	Uniprot
P49459 (UBE2A)	S120	Uniprot
P49736 (MCM2)	S13	Uniprot
P49736 (MCM2)	S27	Uniprot
P49736 (MCM2)	S40	Uniprot
P49736 (MCM2)	S41	Uniprot
P49736 (MCM2)	S53	Uniprot
P49736 (MCM2)	S108	Uniprot
P49736 (MCM2)	S139	Uniprot
P49790 (NUP153)	S386	Uniprot
P49790 (NUP153)	T388	Uniprot
P49915 (GMPS)	S313	Uniprot
P49915 (GMPS)	T318	Uniprot
P49916 (LIG3)	T191	Uniprot
P49916 (LIG3)	S210	Uniprot
P49916 (LIG3)	S913	Uniprot
P50570 (DNM2)	S764	Uniprot
P50613 (CDK7)	S164	Uniprot
P50613 (CDK7)	T170	Uniprot
P50750 (CDK9)	S90	Uniprot
P51649 (ALDH5A1)	T181	Uniprot
P52948 (NUP98)	S612	Uniprot
P54132 (BLM)	S175	Uniprot
P54198-1 (HIRA)	T555	Uniprot
P54198 (HIRA)	T583	Uniprot
P54198 (HIRA)	S584	Uniprot
P54198 (HIRA)	T586	Uniprot
P54198 (HIRA)	T588	Uniprot
P54198-1 (HIRA)	S687	Uniprot
P55196 (AFDN)	T1352	Uniprot
P55273 (CDKN2D)	S76	Uniprot
P55273 (CDKN2D)	T141	Uniprot
P55795 (HNRNPH2)	S104	Uniprot
P57740 (NUP107)	S10	Uniprot
P57740 (NUP107)	S11	Uniprot
P61978 (HNRNPK)	S216	Uniprot
P62136 (PPP1CA)	T320	Uniprot
P62324 (BTG1)	S159	Uniprot
P62913 (RPL11)	T47	Uniprot
P78317 (RNF4)	T26	Uniprot
P78317 (RNF4)	T112	Uniprot
P78347 (GTF2I)	T556	Uniprot
P78347 (GTF2I)	T558	Uniprot
P82970 (HMGN5)	T31	Uniprot
P84022-1 (SMAD3)	T8	Uniprot
P84022-1 (SMAD3)	T179	Uniprot
P84022-1 (SMAD3)	S204	Uniprot
P84022-1 (SMAD3)	S208	Uniprot
P84022-1 (SMAD3)	S213	Uniprot
Q00059 (TFAM)	T122	Uniprot
Q00059 (TFAM)	S124	Uniprot
Q00536 (CDK16)	S138	Uniprot
Q01167 (FOXK2)	S373	Uniprot
Q01167 (FOXK2)	S428	Uniprot
Q01196 (RUNX1)	S249	Uniprot
Q01196 (RUNX1)	S266	Uniprot
Q01196 (RUNX1)	T273	Uniprot
Q01196-8 (RUNX1)	S276	Uniprot
Q01196-8 (RUNX1)	S293	Uniprot
Q01196-8 (RUNX1)	T300	Uniprot
Q01196-8 (RUNX1)	S303	Uniprot
Q02363 (ID2)	S5	Uniprot
Q02535 (ID3)	S5	Uniprot
Q02539 (HIST1H1A)	T152	Uniprot
Q03252 (LMNB2)	S37	Uniprot
Q03252 (LMNB2)	T39	Uniprot
Q04323 (UBXN1)	S199	Uniprot
Q04323 (UBXN1)	S200	Uniprot
Q04323 (UBXN1)	S202	Uniprot
Q04637 (EIF4G1)	T647	Uniprot
Q05516 (ZBTB16)	S197	Uniprot
Q05516 (ZBTB16)	T282	Uniprot
Q05519 (SRSF11)	S207	Uniprot
Q05519 (SRSF11)	S209	Uniprot
Q05682 (CALD1)	T730	Uniprot
Q06830 (PRDX1)	T90	Uniprot
Q07820 (MCL1)	S64	Uniprot
Q07820 (MCL1)	T92	Uniprot
Q07820 (MCL1)	S121	Uniprot
Q07820 (MCL1)	T163	Uniprot
Q08050-2 (FOXM1)	T596	Uniprot
Q08050-1 (FOXM1)	T611	Uniprot
Q08050-3 (FOXM1)	T649	Uniprot
Q08999 (RBL2)	S413	Uniprot
Q08999 (RBL2)	T417	Uniprot
Q08999 (RBL2)	S639	Uniprot
Q08999 (RBL2)	T642	Uniprot
Q08999 (RBL2)	S662	Uniprot
Q08999 (RBL2)	S688	Uniprot
Q08999 (RBL2)	T694	Uniprot
Q08999 (RBL2)	S952	Uniprot
Q08999 (RBL2)	S1044	Uniprot
Q08999 (RBL2)	S1068	Uniprot
Q08999 (RBL2)	S1080	Uniprot
Q08999 (RBL2)	T1097	Uniprot
Q08999 (RBL2)	S1112	Uniprot
Q0VD86 (INCA1)	S23	Uniprot
Q0VD86 (INCA1)	T182	Uniprot
Q0VD86 (INCA1)	S191	Uniprot
Q12778 (FOXO1)	S249	Uniprot
Q12800 (TFCP2)	T258	Uniprot
Q12800 (TFCP2)	S289	Uniprot
Q12800 (TFCP2)	S291	Uniprot
Q12800 (TFCP2)	S309	Uniprot
Q12888 (TP53BP1)	T1609	Uniprot
Q12888 (TP53BP1)	T1612	Uniprot
Q12906 (ILF3)	T363	Uniprot
Q12906 (ILF3)	T364	Uniprot
Q12906 (ILF3)	T368	Uniprot
Q12906 (ILF3)	S382	Uniprot
Q12906 (ILF3)	S384	Uniprot
Q12948 (FOXC1)	T68	Uniprot
Q12959 (DLG1)	S158	Uniprot
Q12959 (DLG1)	S443	Uniprot
Q13118 (KLF10)	S206	Uniprot
Q13242 (SRSF9)	S216	Uniprot
Q13309 (SKP2)	S64	Uniprot
Q13330 (MTA1)	S522	Uniprot
Q13352 (ITGB3BP)	S33	Uniprot
Q13352 (ITGB3BP)	S46	Uniprot
Q13415 (ORC1)	S258	Uniprot
Q13415 (ORC1)	S273	Uniprot
Q13415 (ORC1)	T375	Uniprot
Q13416 (ORC2)	T116	Uniprot
Q13416 (ORC2)	T226	Uniprot
Q13428 (TCOF1)	S582	Uniprot
Q13428 (TCOF1)	S583	Uniprot
Q13428 (TCOF1)	S982	Uniprot
Q13428 (TCOF1)	T983	Uniprot
Q13573 (SNW1)	S224	Uniprot
Q13573 (SNW1)	S232	Uniprot
Q13573 (SNW1)	S234	Uniprot
Q14103 (HNRNPD)	T193	Uniprot
Q14194 (CRMP1)	T509	Uniprot
Q14195 (DPYSL3)	T507	Uniprot
Q14195 (DPYSL3)	T508	Uniprot
Q14195 (DPYSL3)	T509	Uniprot
Q14202 (ZMYM3)	T826	Uniprot
Q14207 (NPAT)	S775	Uniprot
Q14207 (NPAT)	S779	Uniprot
Q14207 (NPAT)	S1100	Uniprot
Q14207 (NPAT)	T1270	Uniprot
Q14207 (NPAT)	T1350	Uniprot
Q14247 (CTTN)	S405	Uniprot
Q14676 (MDC1)	T341	Uniprot
Q14676 (MDC1)	S1068	Uniprot
Q14676 (MDC1)	T1630	Uniprot
Q14807 (KIF22)	S462	Uniprot
Q14807 (KIF22)	T463	Uniprot
Q14839 (CHD4)	T529	Uniprot
Q14839 (CHD4)	S531	Uniprot
Q14980 (NUMA1)	T1776	Uniprot
Q14CB8 (ARHGAP19)	T476	Uniprot
Q14CB8 (ARHGAP19)	T478	Uniprot
Q15003 (NCAPH)	T49	Uniprot
Q15047 (SETDB1)	S1066	Uniprot
Q15398 (DLGAP5)	T342	Uniprot
Q15398 (DLGAP5)	T344	Uniprot
Q15554 (TERF2)	S365	Uniprot
Q15648 (MED1)	S1207	Uniprot
Q15648 (MED1)	T1215	Uniprot
Q15717 (ELAVL1)	S202	Uniprot
Q15796 (SMAD2)	T8	Uniprot
Q15813 (TBCE)	S495	Uniprot
Q15910 (EZH2)	T345	Uniprot
Q15910 (EZH2)	T416	Uniprot
Q15942 (ZYX)	S303	Uniprot
Q15942 (ZYX)	T306	Uniprot
Q15942 (ZYX)	S308	Uniprot
Q16204 (CCDC6)	S244	Uniprot
Q2NL82 (TSR1)	T490	Uniprot
Q32P44 (EML3)	T881	Uniprot
Q32P44 (EML3)	S883	Uniprot
Q4VCS5 (AMOT)	S847	Uniprot
Q4VCS5 (AMOT)	T848	Uniprot
Q4VCS5 (AMOT)	S852	Uniprot
Q4VCS5 (AMOT)	S855	Uniprot
Q53ET0 (CRTC2)	S433	Uniprot
Q53GL7 (PARP10)	T101	Uniprot
Q53H80 (AKIRIN2)	S18	Uniprot
Q53H80 (AKIRIN2)	S21	Uniprot
Q5JTV8 (TOR1AIP1)	S143	Uniprot
Q5JTZ9 (AARS2)	T673	Uniprot
Q5T5Y3 (CAMSAP1)	S1080	Uniprot
Q5TZA2-2 (CROCC)	S763	Uniprot
Q5TZA2 (CROCC)	S1460	Uniprot
Q6P1N0 (CC2D1A)	S208	Uniprot
Q6PKG0 (LARP1)	T303	Uniprot
Q6PKG0 (LARP1)	T649	Uniprot
Q6PKG0 (LARP1)	S774	Uniprot
Q6UB35 (MTHFD1L)	S357	Uniprot
Q6Y7W6-3 (GIGYF2)	S30	Uniprot
Q6Y7W6-3 (GIGYF2)	S614	Uniprot
Q71UM5 (RPS27L)	S27	Uniprot
Q7KZ85 (SUPT6H)	T1523	Uniprot
Q7Z2Z1 (TICRR)	T969	Uniprot
Q7Z2Z1 (TICRR)	S1001	Uniprot
Q7Z417 (NUFIP2)	T219	Uniprot
Q7Z417 (NUFIP2)	T220	Uniprot
Q86T82 (USP37)	S628	Uniprot
Q86U86 (PBRM1)	S25	Uniprot
Q86U86 (PBRM1)	S27	Uniprot
Q86U86 (PBRM1)	T28	Uniprot
Q86U86 (PBRM1)	S32	Uniprot
Q86VM9 (ZC3H18)	S613	Uniprot
Q86VM9 (ZC3H18)	S618	Uniprot
Q86VM9 (ZC3H18)	S795	Uniprot
Q86VM9 (ZC3H18)	T796	Uniprot
Q86WB0 (ZC3HC1)	S394	Uniprot
Q86WB0 (ZC3HC1)	S395	Uniprot
Q86XL3 (ANKLE2)	S528	Uniprot
Q86YP4 (GATAD2A)	S598	Uniprot
Q86YP4 (GATAD2A)	S600	Uniprot
Q8IWS0 (PHF6)	S154	Uniprot
Q8IWS0 (PHF6)	S155	Uniprot
Q8IXJ6-2 (SIRT2)	S331	Uniprot
Q8IXJ6 (SIRT2)	S368	Uniprot
Q8IXM2 (BAP18)	S96	Uniprot
Q8IXM2 (BAP18)	S106	Uniprot
Q8IXM2 (BAP18)	S110	Uniprot
Q8IXQ3 (C9orf40)	S67	Uniprot
Q8IXQ3 (C9orf40)	S69	Uniprot
Q8IXQ3 (C9orf40)	S71	Uniprot
Q8IYB3 (SRRM1)	S769	Uniprot
Q8IYB3 (SRRM1)	S773	Uniprot
Q8IZL8 (PELP1)	S477	Uniprot
Q8IZL8 (PELP1)	S991	Uniprot
Q8N3F8 (MICALL1)	T469	Uniprot
Q8N3F8 (MICALL1)	S470	Uniprot
Q8N3F8 (MICALL1)	S471	Uniprot
Q8N3F8 (MICALL1)	S619	Uniprot
Q8N3F8 (MICALL1)	S620	Uniprot
Q8N3F8 (MICALL1)	S621	Uniprot
Q8NBZ0 (INO80E)	S154	Uniprot
Q8NFC6 (BOD1L1)	S2905	Uniprot
Q8NFC6 (BOD1L1)	S2907	Uniprot
Q8NFC6 (BOD1L1)	S2908	Uniprot
Q8NFC6 (BOD1L1)	S2909	Uniprot
Q8TAP6 (CEP76)	S83	Uniprot
Q8TAP8 (PPP1R35)	S45	Uniprot
Q8TAP8 (PPP1R35)	S47	Uniprot
Q8TAP8 (PPP1R35)	S52	Uniprot
Q8WUM0 (NUP133)	S27	Uniprot
Q8WUM0 (NUP133)	T28	Uniprot
Q8WUM0 (NUP133)	S50	Uniprot
Q8WWY3 (PRPF31)	S445	Uniprot
Q8WWY3 (PRPF31)	S446	Uniprot
Q8WWY3 (PRPF31)	S450	Uniprot
Q8WWY3 (PRPF31)	S451	Uniprot
Q8WWY3 (PRPF31)	T455	Uniprot
Q8WXE1 (ATRIP)	S224	Uniprot
Q8WXE1 (ATRIP)	S239	Uniprot
Q8WYH8 (ING5)	T152	Uniprot
Q92547 (TOPBP1)	T848	Uniprot
Q92620 (DHX38)	S232	Uniprot
Q92620 (DHX38)	S234	Uniprot
Q92785 (DPF2)	T176	Uniprot
Q92785 (DPF2)	T248	Uniprot
Q92785 (DPF2)	S251	Uniprot
Q969V6 (MRTFA)	T573	Uniprot
Q96C19 (EFHD2)	S74	Uniprot
Q96C19 (EFHD2)	S76	Uniprot
Q96C36 (PYCR2)	T301	Uniprot
Q96C36 (PYCR2)	S303	Uniprot
Q96C36 (PYCR2)	S304	Uniprot
Q96E09 (FAM122A)	S143	Uniprot
Q96EA4 (SPDL1)	S513	Uniprot
Q96EA4 (SPDL1)	S515	Uniprot
Q96II8 (LRCH3)	S419	Uniprot
Q96KS0 (EGLN2)	S130	Uniprot
Q96RK0 (CIC)	T583	Uniprot
Q96RK0 (CIC)	S585	Uniprot
Q96T88 (UHRF1)	S661	Uniprot
Q96T88-2 (UHRF1)	S674	Uniprot
Q99459 (CDC5L)	S410	Uniprot
Q99459 (CDC5L)	T411	Uniprot
Q99504 (EYA3)	S266	Uniprot
Q99504 (EYA3)	T268	Uniprot
Q99504 (EYA3)	T269	Uniprot
Q99504 (EYA3)	S271	Uniprot
Q99607 (ELF4)	T643	Uniprot
Q99607 (ELF4)	S648	Uniprot
Q99626 (CDX2)	S283	Uniprot
Q99626 (CDX2)	S287	Uniprot
Q99626 (CDX2)	S291	Uniprot
Q99638 (RAD9A)	S328	Uniprot
Q99708 (RBBP8)	T315	Uniprot
Q99708 (RBBP8)	T847	Uniprot
Q99728 (BARD1)	S148	Uniprot
Q99728 (BARD1)	S251	Uniprot
Q99728 (BARD1)	S288	Uniprot
Q99728 (BARD1)	T299	Uniprot
Q99741 (CDC6)	S54	Uniprot
Q99741 (CDC6)	S74	Uniprot
Q99741 (CDC6)	S106	Uniprot
Q9BUJ2 (HNRNPUL1)	S716	Uniprot
Q9BUJ2 (HNRNPUL1)	S718	Uniprot
Q9BVC5 (C2orf49)	S193	Uniprot
Q9BVC5 (C2orf49)	T197	Uniprot
Q9BVC5 (C2orf49)	T198	Uniprot
Q9BXR0 (QTRT1)	S139	Uniprot
Q9BY44 (EIF2A)	S517	Uniprot
Q9BY44 (EIF2A)	T518	Uniprot
Q9BZL4 (PPP1R12C)	S509	Uniprot
Q9C0C2 (TNKS1BP1)	S435	Uniprot
Q9C0C2 (TNKS1BP1)	S437	Uniprot
Q9C0C2 (TNKS1BP1)	S691	Uniprot
Q9C0C9 (UBE2O)	S836	Uniprot
Q9C0D5 (TANC1)	S1564	Uniprot
Q9H1E3 (NUCKS1)	S181	Uniprot
Q9H211 (CDT1)	T29	Uniprot
Q9H211 (CDT1)	S31	Uniprot
Q9H2G4 (TSPYL2)	S20	Uniprot
Q9H2G4 (TSPYL2)	T340	Uniprot
Q9H3D4 (TP63)	S477	Uniprot
Q9H3D4 (TP63)	T491	Uniprot
Q9H3D4 (TP63)	S560	Uniprot
Q9H3P2 (NELFA)	T157	Uniprot
Q9H425 (C1orf198)	S37	Uniprot
Q9H6K1 (ILRUN)	S215	Uniprot
Q9H7L9 (SUDS3)	S234	Uniprot
Q9H7L9 (SUDS3)	S236	Uniprot
Q9H7L9 (SUDS3)	S237	Uniprot
Q9H7L9 (SUDS3)	T244	Uniprot
Q9H8Y8 (GORASP2)	T225	Uniprot
Q9H910 (JPT2)	S97	Uniprot
Q9H9C1 (VIPAS39)	S130	Uniprot
Q9H9C1 (VIPAS39)	T132	Uniprot
Q9NP87 (POLM)	S372	Uniprot
Q9NPF5 (DMAP1)	T445	Uniprot
Q9NPF5 (DMAP1)	S448	Uniprot
Q9NS91 (RAD18)	S99	Uniprot
Q9NSV4 (DIAPH3)	T19	Uniprot
Q9NTI5 (PDS5B)	S1369	Uniprot
Q9NTI5 (PDS5B)	T1370	Uniprot
Q9NXR1 (NDE1)	T228	Uniprot
Q9NYB0 (TERF2IP)	S203	Uniprot
Q9NYV6 (RRN3)	S44	Uniprot
Q9NZJ0 (DTL)	S428	Uniprot
Q9NZJ0 (DTL)	T429	Uniprot
Q9P270 (SLAIN2)	S48	Uniprot
Q9P2B4 (CTTNBP2NL)	S563	Uniprot
Q9P2D1 (CHD7)	S2471	Uniprot
Q9P2D1 (CHD7)	T2472	Uniprot
Q9P2N5 (RBM27)	T796	Uniprot
Q9P2N5 (RBM27)	S798	Uniprot
Q9UEY8 (ADD3)	S423	Uniprot
Q9UFC0 (LRWD1)	S243	Uniprot
Q9UFC0 (LRWD1)	S245	Uniprot
Q9UGP5 (POLL)	S167	Uniprot
Q9UGP5 (POLL)	S177	Uniprot
Q9UGP5 (POLL)	S230	Uniprot
Q9UGP5 (POLL)	T553	Uniprot
Q9UIF9 (BAZ2A)	S509	Uniprot
Q9UIF9 (BAZ2A)	S1183	Uniprot
Q9UIF9 (BAZ2A)	S1184	Uniprot
Q9UJU6 (DBNL)	S274	Uniprot
Q9UJU6 (DBNL)	S275	Uniprot
Q9UKM9 (RALY)	S135	Uniprot
Q9UKY1 (ZHX1)	T568	Uniprot
Q9ULW0 (TPX2)	T72	Uniprot
Q9ULX3 (NOB1)	T121	Uniprot
Q9UM11 (FZR1)	S40	Uniprot
Q9UM11 (FZR1)	T121	Uniprot
Q9UM11 (FZR1)	S151	Uniprot
Q9UM11 (FZR1)	S163	Uniprot
Q9UPN3 (MACF1)	T7213	Uniprot
Q9UQR0 (SCML2)	T305	Uniprot
Q9UQR0 (SCML2)	S511	Uniprot
Q9UQR0 (SCML2)	S590	Uniprot
Q9Y266 (NUDC)	S136	Uniprot
Q9Y266 (NUDC)	S139	Uniprot
Q9Y2J2 (EPB41L3)	T466	Uniprot
Q9Y2J2 (EPB41L3)	T467	Uniprot
Q9Y2J2 (EPB41L3)	S960	Uniprot
Q9Y2J2 (EPB41L3)	S962	Uniprot
Q9Y2K7 (KDM2A)	T550	Uniprot
Q9Y2W1 (THRAP3)	T874	Uniprot
Q9Y314 (NOSIP)	S166	Uniprot
Q9Y314 (NOSIP)	T168	Uniprot
Q9Y3Z3 (SAMHD1)	T592	Uniprot
Q9Y676 (MRPS18B)	S49	Uniprot

研究背景

P06493_CDK1_HUMAN
P24941_CDK2_HUMAN

機能:

Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. Required in higher cells for entry into S-phase and mitosis. Phosphorylates PARVA/actopaxin, APC, AMPH, APC, BARD1, Bcl-xL/BCL2L1, BRCA2, CALD1, CASP8, CDC7, CDC20, CDC25A, CDC25C, CC2D1A, CENPA, CSNK2 proteins/CKII, FZR1/CDH1, CDK7, CEBPB, CHAMP1, DMD/dystrophin, EEF1 proteins/EF-1, EZH2, KIF11/EG5, EGFR, FANCG, FOS, GFAP, GOLGA2/GM130, GRASP1, UBE2A/hHR6A, HIST1H1 proteins/histone H1, HMGA1, HIVEP3/KRC, LMNA, LMNB, LMNC, LBR, LATS1, MAP1B, MAP4, MARCKS, MCM2, MCM4, MKLP1, MYB, NEFH, NFIC, NPC/nuclear pore complex, PITPNM1/NIR2, NPM1, NCL, NUCKS1, NPM1/numatrin, ORC1, PRKAR2A, EEF1E1/p18, EIF3F/p47, p53/TP53, NONO/p54NRB, PAPOLA, PLEC/plectin, RB1, TPPP, UL40/R2, RAB4A, RAP1GAP, RCC1, RPS6KB1/S6K1, KHDRBS1/SAM68, ESPL1, SKI, BIRC5/survivin, STIP1, TEX14, beta-tubulins, MAPT/TAU, NEDD1, VIM/vimentin, TK1, FOXO1, RUNX1/AML1, SAMHD1, SIRT2 and RUNX2. CDK1/CDC2-cyclin-B controls pronuclear union in interphase fertilized eggs. Essential for early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates CDK1/cyclin complexes which phosphorylate several substrates that trigger at least centrosome separation, Golgi dynamics, nuclear envelope breakdown and chromosome condensation. Once chromosomes are condensed and aligned at the metaphase plate, CDK1 activity is switched off by WEE1- and PKMYT1-mediated phosphorylation to allow sister chromatid separation, chromosome decondensation, reformation of the nuclear envelope and cytokinesis. Inactivated by PKR/EIF2AK2- and WEE1-mediated phosphorylation upon DNA damage to stop cell cycle and genome replication at the G2 checkpoint thus facilitating DNA repair. Reactivated after successful DNA repair through WIP1-dependent signaling leading to CDC25A/B/C-mediated dephosphorylation and restoring cell cycle progression. In proliferating cells, CDK1-mediated FOXO1 phosphorylation at the G2-M phase represses FOXO1 interaction with 14-3-3 proteins and thereby promotes FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. The phosphorylation of beta-tubulins regulates microtubule dynamics during mitosis. NEDD1 phosphorylation promotes PLK1-mediated NEDD1 phosphorylation and subsequent targeting of the gamma-tubulin ring complex (gTuRC) to the centrosome, an important step for spindle formation. In addition, CC2D1A phosphorylation regulates CC2D1A spindle pole localization and association with SCC1/RAD21 and centriole cohesion during mitosis. The phosphorylation of Bcl-xL/BCL2L1 after prolongated G2 arrest upon DNA damage triggers apoptosis. In contrast, CASP8 phosphorylation during mitosis prevents its activation by proteolysis and subsequent apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. CALD1 phosphorylation promotes Schwann cell migration during peripheral nerve regeneration. CDK1-cyclin-B complex phosphorylates NCKAP5L and mediates its dissociation from centrosomes during mitosis. Regulates the amplitude of the cyclic expression of the core clock gene ARNTL/BMAL1 by phosphorylating its transcriptional repressor NR1D1, and this phosphorylation is necessary for SCF(FBXW7)-mediated ubiquitination and proteasomal degradation of NR1D1.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry.

PTMs:

Phosphorylation at Thr-161 by CAK/CDK7 activates kinase activity. Phosphorylation at Thr-14 and Tyr-15 by PKMYT1 prevents nuclear translocation. Phosphorylation at Tyr-15 by WEE1 and WEE2 inhibits the protein kinase activity and acts as a negative regulator of entry into mitosis (G2 to M transition). Phosphorylation by PKMYT1 and WEE1 takes place during mitosis to keep CDK1-cyclin-B complexes inactive until the end of G2. By the end of G2, PKMYT1 and WEE1 are inactivated, but CDC25A and CDC25B are activated. Dephosphorylation by active CDC25A and CDC25B at Thr-14 and Tyr-15, leads to CDK1 activation at the G2-M transition. Phosphorylation at Tyr-15 by WEE2 during oogenesis is required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, leading to prevent meiotic reentry. Phosphorylation by WEE2 is also required for metaphase II exit during egg activation to ensure exit from meiosis in oocytes and promote pronuclear formation. Phosphorylated at Tyr-4 by PKR/EIF2AK2 upon genotoxic stress. This phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest. In response to UV irradiation, phosphorylation at Tyr-15 by PRKCD activates the G2/M DNA damage checkpoint.

Polyubiquitinated upon genotoxic stress.

細胞の位置付け:

Nucleus. Cytoplasm. Mitochondrion. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle.
Note: Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.

組織特異性:

Isoform 2 is found in breast cancer tissues.

サブユニット構造:

Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cyclins-B (CCNB1, CCNB2 and CCNB3) to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Can also form CDK1-cylin-D and CDK1-cyclin-E complexes that phosphorylate RB1 in vitro. Binds to RB1 and other transcription factors such as FOXO1 and RUNX2. Promotes G2-M transition when in complex with a cyclin-B. Interacts with DLGAP5. Binds to the CDK inhibitors CDKN1A/p21 and CDKN1B/p27. Isoform 2 is unable to complex with cyclin-B1 and also fails to bind to CDKN1A/p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase. Associates with cyclins-A and B1 during S-phase in regenerating hepatocytes. Interacts with FANCC. Interacts with CEP63; this interaction recruits CDK1 to centrosomes. Interacts with CENPA. Interacts with NR1D1. Interacts with proteasome subunit PSMA8; to participate in meiosis progression during spermatogenesis (By similarity).

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

機能:

Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC. Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis. In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation. Phosphorylation of RB1 disturbs its interaction with E2F1. NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication. Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase. Required for vitamin D-mediated growth inhibition by being itself inactivated. Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner. USP37 is activated by phosphorylation and thus triggers G1-S transition. CTNNB1 phosphorylation regulates insulin internalization. Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates CDK2AP2. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks.

PTMs:

Phosphorylated at Thr-160 by CDK7 in a CAK complex. Phosphorylation at Thr-160 promotes kinase activity, whereas phosphorylation at Tyr-15 by WEE1 reduces slightly kinase activity. Phosphorylated on Thr-14 and Tyr-15 during S and G2 phases before being dephosphorylated by CDC25A.

Nitrosylated after treatment with nitric oxide (DETA-NO).

細胞の位置付け:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus>Cajal body. Cytoplasm. Endosome.
Note: Localized at the centrosomes in late G2 phase after separation of the centrosomes but before the start of prophase. Nuclear-cytoplasmic trafficking is mediated during the inhibition by 1,25-(OH)(2)D(3).

サブユニット構造:

Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Interaction with SPDYA promotes kinase activation via a conformation change that alleviates obstruction of the substrate-binding cleft by the T-loop. Found in a complex with both SPDYA and CDKN1B/KIP1. Binds to RB1 and CDK7. Binding to CDKN1A (p21) leads to CDK2/cyclin E inactivation at the G1-S phase DNA damage checkpoint, thereby arresting cells at the G1-S transition during DNA repair. Associated with PTPN6 and beta-catenin/CTNNB1. Interacts with CACUL1. May interact with CEP63. Interacts with ANKRD17. Interacts with CEBPA (when phosphorylated). Forms a ternary complex with CCNA2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with cyclins A, B1, B3, D, or E. Interacts with CDK2AP2.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

研究領域

· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

参考文献

1). Sinomenine Inhibits the Growth of Ovarian Cancer Cells Through the Suppression of Mitosis by Down-Regulating the Expression and the Activity of CDK1. OncoTargets and Therapy, 2021 (PubMed: 33574676) [IF=4.0]

Application: WB Species: Human Sample: HeyA8 cells

Figure 5 Sinomenine suppressed G2/M transition in HeyA8 cells by inhibiting the expression and the activity of CDK1. (A and B) The cell cycle transition of HeyA8 cells was examined by flow cytometry assay (A). The results showed that the percentage of cells in the G2/M phase was decreased significantly after treatment with sinomenine for 48 hours (B). Each value represents the mean ± SD for triplicate samples. ***P<0.001, Student’s t-test. (C and D) The percentage of M phase cells were analyzed by flow cytometry assay staining with P-Histone H3 (Ser10) and PI (C). The results showed that the percentage of M phase in HeyA8 cells treated with sinomenine for 48 hours were obviously decreased (D). Each value represents the mean ± SD for triplicate samples. ***P<0.001, Student’s t-test. (E) The expression of specific proteins in different cell cycle phase were determined by Western blot. (F) The expression level of P-Histone H3 (ser10), which only expressed in the M phase, were clearly decreased in sinomenine treated HeyA8 cells. Each value represents the mean ± SD for triplicate samples. ***P<0.001, one-way ANOVA analysis. (G) The expression of CDK1 and the phosphorylation of CDK1 were detected by Western blot. (H) The expression level CDK1 and P-CDK1 (Thr161) were clearly decreased in sinomenine treated HeyA8 cells. Each value represents the mean ± SD for triplicate samples. **P<0.01, ***P<0.001, one-way ANOVA analysis.

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Phospho-Cdk1/2 (Thr14) Antibody - #DF2944

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