Phospho-Histone H1.3 (Thr18) Antibody - #DF2946

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製品: Phospho-Histone H1.3 (Thr18) Antibody
カタログ: DF2946
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Histone H1.3 (Thr18)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 30kDa; 22kD(Calculated).
ユニプロット: P10412 | P16402
RRID: AB_2840930

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説明書
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プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-Histone H1.3 (Thr18) Antibody detects endogenous levels of Histone H1.3 only when phosphorylated at Thr18.
RRID:
AB_2840930
引用形式: Affinity Biosciences Cat# DF2946, RRID:AB_2840930.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

H1 histone family member 4; H1.4; H14_HUMAN; H1E; H1F4; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; Histone H1; Histone H1.4; Histone H1B; MGC116819; H1 histone family member 3; H1.3; H13_HUMAN; H1F3; HIST1 H1D; HIST1H1D; Histone 1 H1d; Histone cluster 1 H1d; Histone H1.3; Histone H1c; MGC138176;

免疫原

免疫原:

A synthesized peptide derived from human Histone H1.3 around the phosphorylation site of T18.

Uniprot:

P10412(H14_HUMAN) >>Visit HPA database.

P16402(H13_HUMAN) >>Visit HPA database.

遺伝子(ID):
HIST1H1D(3007) | HIST1H1E(3008)
タンパク質配列:
MSETAPAAPAAPAPAEKTPVKKKARKSAGAAKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGAAKKPKKATGAATPKKSAKKTPKKAKKPAAAAGAKKAKSPKKAKAAKPKKAPKSPAKAKAVKPKAAKPKTAKPKAAKPKKAAAKKK

MSETAPLAPTIPAPAEKTPVKKKAKKAGATAGKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEGKPKAKKAGAAKPRKPAGAAKKPKKVAGAATPKKSIKKTPKKVKKPATAAGTKKVAKSAKKVKTPQPKKAAKSPAKAKAPKPKAAKPKSGKPKVTKAKKAAPKKK

PTMs - P10412/P16402 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T4 Phosphorylation
K17 Acetylation
K17 Ubiquitination
T18 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
K21 Ubiquitination
K26 Acetylation
K26 Methylation
S27 Phosphorylation Q96GD4 (AURKB)
K32 Acetylation
K34 Acetylation
K34 Methylation
K34 Ubiquitination
S36 Phosphorylation P17612 (PRKACA)
S41 Phosphorylation
T45 Phosphorylation
K46 Acetylation
K46 Ubiquitination
S51 Phosphorylation
K52 Acetylation
K52 Ubiquitination
R54 Methylation
S55 Phosphorylation
S58 Phosphorylation
K63 Acetylation
K63 Ubiquitination
K64 Methylation
K64 Sumoylation
K64 Ubiquitination
Y71 Phosphorylation
K75 Acetylation
K75 Methylation
K75 Ubiquitination
S78 Phosphorylation
K85 Acetylation
K85 Ubiquitination
S86 Phosphorylation
S89 Phosphorylation
K90 Acetylation
K90 Ubiquitination
T92 Phosphorylation
K97 Methylation
K97 Ubiquitination
T99 Phosphorylation
S102 Phosphorylation
S104 Phosphorylation
K106 Acetylation
K106 Methylation
K106 Ubiquitination
K109 Ubiquitination
K110 Ubiquitination
S113 Phosphorylation
K117 Ubiquitination
K119 Methylation
K119 Ubiquitination
K121 Methylation
K129 Acetylation
K129 Methylation
K130 Acetylation
K136 Acetylation
K136 Ubiquitination
K137 Acetylation
K137 Ubiquitination
K140 Ubiquitination
T142 Phosphorylation
T146 Phosphorylation
K148 Acetylation
K148 Methylation
K148 Ubiquitination
T154 Phosphorylation
K159 Methylation
K159 Ubiquitination
K160 Acetylation
K160 Ubiquitination
K168 Acetylation
K168 Ubiquitination
K169 Acetylation
K171 Methylation
S172 Phosphorylation
K177 Methylation
S187 Phosphorylation
K192 Methylation
K195 Ubiquitination
K197 Ubiquitination
Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T4 Phosphorylation
T10 Phosphorylation
K17 Acetylation
K17 Ubiquitination
T18 Phosphorylation
K33 Acetylation
K35 Acetylation
K35 Methylation
K35 Ubiquitination
S37 Phosphorylation
S42 Phosphorylation
T46 Phosphorylation
K47 Acetylation
K47 Ubiquitination
S52 Phosphorylation
K53 Acetylation
K53 Ubiquitination
R55 Methylation
S56 Phosphorylation
S59 Phosphorylation
K64 Acetylation
K64 Ubiquitination
K65 Methylation
K65 Sumoylation
K65 Ubiquitination
Y72 Phosphorylation
K76 Acetylation
K76 Ubiquitination
S79 Phosphorylation
K86 Acetylation
K86 Ubiquitination
S87 Phosphorylation
S90 Phosphorylation
K91 Acetylation
K91 Ubiquitination
T93 Phosphorylation
K98 Methylation
K98 Ubiquitination
T100 Phosphorylation
S103 Phosphorylation
S105 Phosphorylation
K107 Acetylation
K107 Methylation
K107 Ubiquitination
K110 Ubiquitination
K111 Ubiquitination
K118 Ubiquitination
K141 Ubiquitination
T147 Phosphorylation
K149 Ubiquitination
S151 Phosphorylation
T155 Phosphorylation
K160 Ubiquitination
K161 Ubiquitination
T164 Phosphorylation
K169 Acetylation
K170 Acetylation
K173 Acetylation
K176 Acetylation
K179 Ubiquitination
T180 Phosphorylation
K188 Acetylation
S189 Phosphorylation
K207 Acetylation
K209 Acetylation
K212 Acetylation

研究背景

機能:

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

PTMs:

H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.

Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.

Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.

ADP-ribosylated on Ser-150 in response to DNA damage.

細胞の位置付け:

Nucleus. Chromosome.
Note: Mainly localizes in heterochromatin. Dysplays a punctuate staining pattern in the nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The C-terminal domain is required for high-affinity binding to chromatin.

Belongs to the histone H1/H5 family.

機能:

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

PTMs:

H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.

Citrullination at Arg-55 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.

細胞の位置付け:

Nucleus. Chromosome.
Note: According to PubMed:15911621 more commonly found in euchromatin. According to PubMed:10997781 is associated with inactive chromatin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The C-terminal domain is required for high-affinity binding to chromatin.

Belongs to the histone H1/H5 family.

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