IP3 Receptor Antibody - #DF3000
(1)
(3)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF3000, RRID:AB_2840979.
折りたたみ／展開
4; 5-trisphosphate receptor; 5-trisphosphate receptor type 1; DKFZp313E1334; DKFZp313N1434; inositol 1 4 5 triphosphate receptor type 1; Inositol 1 4 5 trisphosphate Receptor Type 1; Inositol 1; InsP3R1; IP3; IP3 receptor; IP3 receptor isoform 1; IP3R 1; IP3R; IP3R1; ITPR 1; Itpr1; ITPR1_HUMAN; SCA15; SCA16; SCA29; Type 1 inositol 1 4 5 trisphosphate receptor; Type 1 inositol 1; Type 1 InsP3 receptor;
免疫原
- Q14643 ITPR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSDKMSSFLHIGDICSLYAEGSTNGFISTLGLVDDRCVVQPETGDLNNPPKKFRDCLFKLCPMNRYSAQKQFWKAAKPGANSTTDAVLLNKLHHAADLEKKQNETENRKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDEAGNEGSWFYIQPFYKLRSIGDSVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCNTSWKIVLFMKWSDNKDDILKGGDVVRLFHAEQEKFLTCDEHRKKQHVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVDPDFEEECLEFQPSVDPDQDASRSRLRNAQEKMVYSLVSVPEGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNIPIDKEEEKPVMLKIGTSPVKEDKEAFAIVPVSPAEVRDLDFANDASKVLGSIAGKLEKGTITQNERRSVTKLLEDLVYFVTGGTNSGQDVLEVVFSKPNRERQKLMREQNILKQIFKLLQAPFTDCGDGPMLRLEELGDQRHAPFRHICRLCYRVLRHSQQDYRKNQEYIAKQFGFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICKAVLNPTNADILIETKLVLSRFEFEGVSSTGENALEAGEDEEEVWLFWRDSNKEIRSKSVRELAQDAKEGQKEDRDVLSYYRYQLNLFARMCLDRQYLAINEISGQLDVDLILRCMSDENLPYDLRASFCRLMLHMHVDRDPQEQVTPVKYARLWSEIPSEIAIDDYDSSGASKDEIKERFAQTMEFVEEYLRDVVCQRFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHVTTIFPISKMAKGEENKGNNDVEKLKSSNVMRSIHGVGELMTQVVLRGGGFLPMTPMAAAPEGNVKQAEPEKEDIMVMDTKLKIIEILQFILNVRLDYRISCLLCIFKREFDESNSQTSETSSGNSSQEGPSNVPGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSGALQLLFRHFSQRQEVLQAFKQVQLLVTSQDVDNYKQIKQDLDQLRSIVEKSELWVYKGQGPDETMDGASGENEHKKTEEGNNKPQKHESTSSYNYRVVKEILIRLSKLCVQESASVRKSRKQQQRLLRNMGAHAVVLELLQIPYEKAEDTKMQEIMRLAHEFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRNVQYIKFLQTIVKAEGKFIKKCQDMVMAELVNSGEDVLVFYNDRASFQTLIQMMRSERDRMDENSPLMYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKIAYINFLNHCYVDTEVEMKEIYTSNHMWKLFENFLVDICRACNNTSDRKHADSILEKYVTEIVMSIVTTFFSSPFSDQSTTLQTRQPVFVQLLQGVFRVYHCNWLMPSQKASVESCIRVLSDVAKSRAIAIPVDLDSQVNNLFLKSHSIVQKTAMNWRLSARNAARRDSVLAASRDYRNIIERLQDIVSALEDRLRPLVQAELSVLVDVLHRPELLFPENTDARRKCESGGFICKLIKHTKQLLEENEEKLCIKVLQTLREMMTKDRGYGEKLISIDELDNAELPPAPDSENATEELEPSPPLRQLEDHKRGEALRQVLVNRYYGNVRPSGRRESLTSFGNGPLSAGGPGKPGGGGGGSGSSSMSRGEMSLAEVQCHLDKEGASNLVIDLIMNASSDRVFHESILLAIALLEGGNTTIQHSFFCRLTEDKKSEKFFKVFYDRMKVAQQEIKATVTVNTSDLGNKKKDDEVDRDAPSRKKAKEPTTQITEEVRDQLLEASAATRKAFTTFRREADPDDHYQPGEGTQATADKAKDDLEMSAVITIMQPILRFLQLLCENHNRDLQNFLRCQNNKTNYNLVCETLQFLDCICGSTTGGLGLLGLYINEKNVALINQTLESLTEYCQGPCHENQNCIATHESNGIDIITALILNDINPLGKKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVIKKAYMQGEVEFEDGENGEDGAASPRNVGHNIYILAHQLARHNKELQSMLKPGGQVDGDEALEFYAKHTAQIEIVRLDRTMEQIVFPVPSICEFLTKESKLRIYYTTERDEQGSKINDFFLRSEDLFNEMNWQKKLRAQPVLYWCARNMSFWSSISFNLAVLMNLLVAFFYPFKGVRGGTLEPHWSGLLWTAMLISLAIVIALPKPHGIRALIASTILRLIFSVGLQPTLFLLGAFNVCNKIIFLMSFVGNCGTFTRGYRAMVLDVEFLYHLLYLVICAMGLFVHEFFYSLLLFDLVYREETLLNVIKSVTRNGRSIILTAVLALILVYLFSIVGYLFFKDDFILEVDRLPNETAVPETGESLASEFLFSDVCRVESGENCSSPAPREELVPAEETEQDKEHTCETLLMCIVTVLSHGLRSGGGVGDVLRKPSKEEPLFAARVIYDLLFFFMVIIIVLNLIFGVIIDTFADLRSEKQKKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNMWHYLCFIVLVKVKDSTEYTGPESYVAEMIKERNLDWFPRMRAMSLVSSDSEGEQNELRNLQEKLESTMKLVTNLSGQLSELKDQMTEQRKQKQRIGLLGHPPHMNVNPQQPA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14643 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K77 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| Y131 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K258 | Acetylation | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| Y353 | Phosphorylation | P06241 (FYN) | Uniprot |
| T420 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K424 | Methylation | Uniprot | |
| K424 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| S436 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K451 | Ubiquitination | Uniprot | |
| K459 | Ubiquitination | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| K569 | Acetylation | Uniprot | |
| K576 | Acetylation | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| T800 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S826 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| T955 | Phosphorylation | Uniprot | |
| K1123 | Ubiquitination | Uniprot | |
| K1126 | Ubiquitination | Uniprot | |
| Y1144 | Phosphorylation | Uniprot | |
| K1145 | Ubiquitination | Uniprot | |
| K1174 | Ubiquitination | Uniprot | |
| T1238 | Phosphorylation | Uniprot | |
| K1327 | Ubiquitination | Uniprot | |
| Y1401 | Phosphorylation | Uniprot | |
| K1405 | Ubiquitination | Uniprot | |
| K1554 | Ubiquitination | Uniprot | |
| K1574 | Ubiquitination | Uniprot | |
| S1577 | Phosphorylation | Uniprot | |
| K1581 | Methylation | Uniprot | |
| S1598 | Phosphorylation | Uniprot | |
| S1603 | Phosphorylation | Uniprot | |
| Y1606 | Phosphorylation | Uniprot | |
| K1664 | Ubiquitination | Uniprot | |
| K1670 | Ubiquitination | Uniprot | |
| T1687 | Phosphorylation | Uniprot | |
| S1759 | Phosphorylation | Uniprot | |
| S1764 | Phosphorylation | Uniprot | |
| T1766 | Phosphorylation | Uniprot | |
| S1767 | Phosphorylation | Uniprot | |
| S1774 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| K1873 | Ubiquitination | Uniprot | |
| T1882 | Phosphorylation | Uniprot | |
| K1893 | Ubiquitination | Uniprot | |
| K1910 | Ubiquitination | Uniprot | |
| K1933 | Ubiquitination | Uniprot | |
| T1937 | Phosphorylation | Uniprot | |
| K2127 | Ubiquitination | Uniprot | |
| K2134 | Ubiquitination | Uniprot | |
| K2229 | Ubiquitination | Uniprot | |
| K2247 | Ubiquitination | Uniprot | |
| S2255 | Phosphorylation | Uniprot | |
| K2266 | Ubiquitination | Uniprot | |
| Y2275 | Phosphorylation | Uniprot | |
| T2434 | Phosphorylation | Uniprot | |
| N2512 | N-Glycosylation | Uniprot | |
| K2616 | Ubiquitination | Uniprot | |
| Y2649 | Phosphorylation | Uniprot | |
| S2661 | Phosphorylation | Uniprot | |
| T2662 | Phosphorylation | Uniprot | |
| K2676 | Ubiquitination | Uniprot | |
| S2690 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S2693 | Phosphorylation | Uniprot | |
| S2694 | Phosphorylation | Uniprot | |
| S2696 | Phosphorylation | Uniprot | |
| K2709 | Ubiquitination | Uniprot | |
| S2721 | Phosphorylation | Uniprot | |
| K2728 | Ubiquitination | Uniprot |
研究背景
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. Involved in the regulation of epithelial secretion of electrolytes and fluid through the interaction with AHCYL1 (By similarity). Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaM kinase II, eventually leading to the activation of downstream apoptosis pathways (By similarity).
Phosphorylated on tyrosine residues.
Ubiquitination at multiple lysines targets ITPR1 for proteasomal degradation. Approximately 40% of the ITPR1-associated ubiquitin is monoubiquitin, and polyubiquitins are both 'Lys-48'- and 'Lys-63'-linked (By similarity).
Phosphorylated by cAMP kinase (PKA). Phosphorylation prevents the ligand-induced opening of the calcium channels. Phosphorylation by PKA increases the interaction with inositol 1,4,5-trisphosphate and decreases the interaction with AHCYL1.
Palmitoylated by ZDHHC6 in immune cells, leading to regulate ITPR1 stability and function.
Endoplasmic reticulum membrane>Multi-pass membrane protein. Cytoplasmic vesicle>Secretory vesicle membrane>Multi-pass membrane protein. Cytoplasm>Perinuclear region.
Note: Endoplasmic reticulum and secretory granules (By similarity).
Widely expressed.
Homotetramer. Interacts with TRPC4. The PPXXF motif binds HOM1, HOM2 and HOM3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. Interacts with ERP44 in a pH-, redox state- and calcium-dependent manner which results in the inhibition the calcium channel activity. The strength of this interaction inversely correlates with calcium concentration. Part of cGMP kinase signaling complex at least composed of ACTA2/alpha-actin, CNN1/calponin H1, PLN/phospholamban, PRKG1 and ITPR1. Interacts with MRVI1 and CABP1 (via N-terminus). Interacts with TESPA1. Interacts (when not phosphorylated) with AHCYL1 (when phosphorylated); the interaction suppresses inositol 1,4,5-trisphosphate binding to ITPR1. Interacts with AHCYL2 (with lower affinity than with AHCYL1). Interacts with BOK (via BH4 domain); protects ITPR1 from proteolysis by CASP3 during apoptosis.
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region.
Belongs to the InsP3 receptor family.
研究領域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > Calcium signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Environmental Information Processing > Signal transduction > Apelin signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Environmental adaptation > Circadian entrainment.
· Organismal Systems > Nervous system > Long-term potentiation.
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
· Organismal Systems > Nervous system > Glutamatergic synapse.
· Organismal Systems > Nervous system > Cholinergic synapse.
· Organismal Systems > Nervous system > Serotonergic synapse.
· Organismal Systems > Nervous system > Dopaminergic synapse.
· Organismal Systems > Nervous system > Long-term depression.
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone synthesis.
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
· Organismal Systems > Endocrine system > Glucagon signaling pathway.
· Organismal Systems > Endocrine system > Renin secretion.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Digestive system > Salivary secretion.
· Organismal Systems > Digestive system > Gastric acid secretion.
· Organismal Systems > Digestive system > Pancreatic secretion.
参考文献
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