CCR2 Antibody - #DF7507

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製品: CCR2 Antibody
カタログ: DF7507
タンパク質の説明: Rabbit polyclonal antibody to CCR2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 42 kDa; 42kD(Calculated).
ユニプロット: P41597
RRID: AB_2841007

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(83%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
CCR2 Antibody detects endogenous levels of total CCR2.
RRID:
AB_2841007
引用形式: Affinity Biosciences Cat# DF7507, RRID:AB_2841007.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C C chemokine receptor type 2; C C CKR 2; C-C chemokine receptor type 2; C-C CKR-2; CC chemokine receptor type 2; CC CKR 2; CC-CKR-2; CCCKR2; CCR 2; CCR-2; CCR1L; CCR2; CCR2_HUMAN; CCR2A; CCR2B; CCR5L; CD192; CD192 antigen; Chemokine C C motif receptor 2; Chemokine CC Motif Receptor 2; CKR 2; CKR2; CKR2A; CKR2B; CMKBR2; MCP 1 R; MCP-1-R; MCP1 RECEPTOR; MCP1R; Monocyte chemoattractant protein 1 receptor; Monocyte Chemotactic Protein 1 Receptor; Monocyte Chemotactic Protein 1 Receptor;

免疫原

免疫原:
Uniprot:

P41597(CCR2_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCR2(729230)
発現特異性:
P41597 CCR2_HUMAN:

Expressed by monocytes and IL2-activated NK cells.

タンパク質配列:
MLSTSRSRFIRNTNESGEEVTTFFDYDYGAPCHKFDVKQIGAQLLPPLYSLVFIFGFVGNMLVVLILINCKKLKCLTDIYLLNLAISDLLFLITLPLWAHSAANEWVFGNAMCKLFTGLYHIGYFGGIFFIILLTIDRYLAIVHAVFALKARTVTFGVVTSVITWLVAVFASVPGIIFTKCQKEDSVYVCGPYFPRGWNNFHTIMRNILGLVLPLLIMVICYSGILKTLLRCRNEKKRHRAVRVIFTIMIVYFLFWTPYNIVILLNTFQEFFGLSNCESTSQLDQATQVTETLGMTHCCINPIIYAFVGEKFRSLFHIALGCRIAPLQKPVCGGPGVRPGKNVKVTTQGLLDGRGKGKSIGRAPEASLQDKEGA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Horse
92
Dog
92
Rabbit
83
Chicken
50
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41597 基板として

Site PTM Type Enzyme
Y139 Phosphorylation O60674 (JAK2)

研究背景

機能:

Key functional receptor for CCL2 but can also bind CCL7 and CCL12. Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (Probable). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B. Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (By similarity). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (By similarity).

(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.

PTMs:

N-glycosylated.

Sulfation increases the affinity for both monomeric and dimeric CCL2 with stronger binding to the monomeric form. Binding of sulfated CCR2 to CCL2 promotes conversion of CCL2 from dimer to monomer.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.
Note: The chemoattractant receptors are distributed throughout the cell surface; after stimulation with a ligand, such as CCL2, they are rapidly recruited into microdomain clusters at the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed by monocytes and IL2-activated NK cells.

サブユニット構造:

Interacts with ARRB1. Interacts (via extracellular N-terminal region) with beta-defensin DEFB106A/DEFB106B; this interaction may preferentially require specific tyrosine sulfation on CCR2. Interacts with NUP85; the interaction is required for CCR2 clusters formation on the cell membrane and CCR2 signaling.

(Microbial infection) Binds to HIV-1 Tat.

タンパク質ファミリー:

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

参考文献

1). Intestinal Flora Changes Induced by a High-Fat Diet Promote Activation of Primordial Follicles through Macrophage Infiltration and Inflammatory Factor Secretion in Mouse Ovaries. International Journal of Molecular Sciences, 2022 (PubMed: 35563189) [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample:

Figure 3 M1 macrophage infiltration into the ovaries is the main reason for the overactivation of primordial follicles in HFD mice. (A) qPCR analysis of the macrophage chemokine receptors in the ovaries of the ND and HFD mice. (B) Immunofluorescence detection of CCR2 (green) and F4/80 (red) in ND, HFD, and HFD+CVC mice (left panel), or in the control, LPS, and LPS+CVC treated mice (right panel). DNA was stained with DAPI (blue). Scale bar = 50 µm. (C) Representative H&E staining images and follicle number counts in the ovaries of HFD mice treated with CVC (n = 3), compared to the HFD mice (n = 5). ND mice were used as controls (n = 5). Scale bar = 50 μm. (D) Representative H&E staining images and follicle number counts in the ovaries of the control, LPS, and LPS+CVC treated mice (n = 5). Scale bar = 50 μm. Red arrows represent primordial follicles. Black arrows represent growing follicles.
2). CCL2/CCR2 Axis Promotes the Progression of Salivary Adenoid Cystic Carcinoma via Recruiting and Reprogramming the Tumor-Associated Macrophages. Frontiers in Oncology, 2019 (PubMed: 31024838) [IF=4.7]

Application: WB    Species: human    Sample: SACC-83 cells and macrophages

FIGURE 3 | SACC cells derived CCL2 activated the expression of CCR2 in TAMs. SACC-83 cells and macrophages were co-cultured to simulate the interactions between SACC cells and TAMs. (A) The concentration of CCL2 in the conditioned media was examined by ELISA. The expression of CCL2 and CCR2 in the solely or co-cultured SACC-83 cells and macrophages were examined by qRT-PCR (B) and western blot (C).

Application: WB    Species: human    Sample: SACC-83 cells

FIGURE 3 | SACC cells derived CCL2 activated the expression of CCR2 in TAMs. SACC-83 cells and macrophages were co-cultured to simulate the interactions between SACC cells and TAMs. (A) The concentration of CCL2 in the conditioned media was examined by ELISA. The expression of CCL2 and CCR2 in the solely or co-cultured SACC-83 cells and macrophages were examined by qRT-PCR (B) and western blot (C).The knockdown effect of CCL2 in SACC-83 cells was measured by qRT-PCR (D), western blot (E), and immunofluorescence (F).

Application: IF/ICC    Species: human    Sample: SACC-83 cells

FIGURE 3 | SACC cells derived CCL2 activated the expression of CCR2 in TAMs. SACC-83 cells and macrophages were co-cultured to simulate the interactions between SACC cells and TAMs. (A) The concentration of CCL2 in the conditioned media was examined by ELISA. The expression of CCL2 and CCR2 in the solely or co-cultured SACC-83 cells and macrophages were examined by qRT-PCR (B) and western blot (C).The knockdown effect of CCL2 in SACC-83 cells was measured by qRT-PCR (D), western blot (E), and immunofluorescence (F).
3). High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells. American Journal of Translational Research, 2019 (PubMed: 30788000) [IF=2.2]

Application: IHC    Species: human    Sample: primary NSCLC and bone metastasis tissues

Figure 2. |Expression of possible target chemokine receptors of CCL7 in primary NSCLC and bone metastasis tissues. A-C. IHC detection of CCR1, CCR2 and CCR3 expression in primary NSCLC and bone metastasis tissues. CCR1, CCR2 and CCR3 were mainly expressed on the cell membrane and in cytoplasm (arrow). D. qRT-PCR measurement of mRNA levels of CCR1, CCR2, CCR3 in primary NSCLC and bone metastasis tissues. *: P < 0.05.

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