GLUT2 Antibody - #DF7510

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製品: GLUT2 Antibody
カタログ: DF7510
タンパク質の説明: Rabbit polyclonal antibody to GLUT2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 57 kDa.; 57kD(Calculated).
ユニプロット: P11168
RRID: AB_2841009

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
GLUT2 Antibody detects endogenous levels of total GLUT2.
RRID:
AB_2841009
引用形式: Affinity Biosciences Cat# DF7510, RRID:AB_2841009.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

liver; Glucose Transporter 2; Glucose Transporter GLUT2; Glucose transporter type 2; Glucose transporter type 2 liver; Glucose transporter, liver/islet; GLUT-2; GLUT2; GTR2_HUMAN; GTT2; SLC2A2; Solute carrier family 2 (facilitated glucose transporter) member 2; Solute carrier family 2 facilitated glucose transporter member 2; Solute carrier family 2, facilitated glucose transporter member 2;

免疫原

免疫原:
Uniprot:

P11168(GTR2_HUMAN) >>Visit HPA database.

遺伝子(ID):
SLC2A2(6514)
発現特異性:
P11168 GTR2_HUMAN:

Liver, insulin-producing beta cell, small intestine and kidney.

タンパク質配列:
MTEDKVTGTLVFTVITAVLGSFQFGYDIGVINAPQQVIISHYRHVLGVPLDDRKAINNYVINSTDELPTISYSMNPKPTPWAEEETVAAAQLITMLWSLSVSSFAVGGMTASFFGGWLGDTLGRIKAMLVANILSLVGALLMGFSKLGPSHILIIAGRSISGLYCGLISGLVPMYIGEIAPTALRGALGTFHQLAIVTGILISQIIGLEFILGNYDLWHILLGLSGVRAILQSLLLFFCPESPRYLYIKLDEEVKAKQSLKRLRGYDDVTKDINEMRKEREEASSEQKVSIIQLFTNSSYRQPILVALMLHVAQQFSGINGIFYYSTSIFQTAGISKPVYATIGVGAVNMVFTAVSVFLVEKAGRRSLFLIGMSGMFVCAIFMSVGLVLLNKFSWMSYVSMIAIFLFVSFFEIGPGPIPWFMVAEFFSQGPRPAALAIAAFSNWTCNFIVALCFQYIADFCGPYVFFLFAGVLLAFTLFTFFKVPETKGKSFEEIAAEFQKKSGSAHRPKAAVEMKFLGATETV

PTMs - P11168 基板として

Site PTM Type Enzyme
S491 Phosphorylation
S503 Phosphorylation
S505 Phosphorylation
T523 Phosphorylation

研究背景

機能:

Facilitative hexose transporter that mediates the transport of glucose and fructose. Likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. Also able to mediate the transport of dehydroascorbate.

PTMs:

N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Liver, insulin-producing beta cell, small intestine and kidney.

タンパク質ファミリー:

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Digestive system > Carbohydrate digestion and absorption.

参考文献

1). Liubao brick tea activates the PI3K-Akt signaling pathway to lower blood glucose, metabolic disorders and insulin resistance via altering the intestinal flora. FOOD RESEARCH INTERNATIONAL, 2021 (PubMed: 34507739) [IF=8.1]

Application: WB    Species: Mice    Sample: liver tissue

Fig. 7. LBT treatment activated the PI3K/Akt pathway in the liver of hyperglycemic and insulin-resistant mice and insulin-resistant HepG2 cells. (A) Hepatic immunohistochemical analysis of PI3K p85, pAkt and Akt protein expressions, (B) Cellular western blotting analysis of GLUT2, PI3K p85 and Akt protein expressions. Different letters represent a significant difference among multiple groups, * represents a significance compared with the Control group, # represents a significance between two groups, ns represents no significance. #,*, p < 0.05; ##, **, p < 0.01.
2). Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice. JCI Insight, 2022 (PubMed: 35077394) [IF=8.0]

Application: WB    Species: Mice    Sample: hippocampal homogenates

Figure 5 RH reduced brain GLUT3-mediated glucose uptake in STZ-induced APP/PS1-DM mice. (A) Representative PET/CT images showing in vivo brain 18F-FDG uptake (coronal, sagittal, and transaxial sections, n = 6 mice for each group). Cor, Cortex; Hip, hippocampus; Mid, midbrain; HPOA, hypothalamus; BS, brain stem; Cer, cerebellum; OB, Olfactory Bulb; BF, basal forebrain; Tha, thalamus; SC, superior colliculi; CG, central gray; Str, striatum. (B) Quantification for SUV of 18F-FDG in brain region (n = 6 for mice for each group). (C) Western blot and quantitation for GLUT1, GLUT2, GLUT3, GLUT4, GLUT5, SGLT1, and SGLT2 in hippocampal homogenates (n = 3 mice for each group). The data are expressed as the mean ± SEM. Statistical significance was assessed using unpaired Student’s t test. *P < 0.05 and **P < 0.01, APP/PS1-DM versus APP/PS1; #P < 0.05 and ##P < 0.01, APP/PS1-DM-RH versus APP/PS1-DM; WT versus APP/PS1; NS, no significant difference.
3). Network pharmacology-based elucidation of bioactive compounds and experimental exploration of antidiabetic mechanisms of Hydrolea zeylanica. Cellular signalling, 2024 (PubMed: 38052370) [IF=4.8]
4). The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis. EXPERIMENTAL CELL RESEARCH, 2019 (PubMed: 31202710) [IF=3.7]

Application: WB    Species: mouse    Sample: MIN6-PC and MIN6-MKP5 cells

Fig. 3. |Cell dysfunction caused by GP is improved in MKP-5 overexpressing MIN6 cells. (B, C) MIN6-PC and MIN6-MKP5 cells were stimulated with GP for 24 h. The phosphorylation of AKT and the expression of GLUT-2 were assessed by western blotting (B). Relative levels of PDX-1 and GCK mRNA were quantified by real-time PCR analysis (C). Data are given as means ± SEM. *, P < 0.05.
5). Identification of Key Candidate Genes and Pathways of Candida albicans-Infected Human Umbilical Vein Endothelial Cells and Drug Screening. INDIAN JOURNAL OF MICROBIOLOGY, 2020 (PubMed: 32089575) [IF=3.0]

Application: WB    Species: human    Sample:

Fig. 3 Receptor–ligand interactions of compound. a Protocol flowchart of MYC inhibitor discovery strategy, ten candidate inhibitors including okanin were screened at last through HTV, SP,and XP screen methods. b Binding model of MYC–DNA complexes with okanin (PDB code: 1NKP) through molecular docking method.The results showed that okanin interacted with two key amino acid residues in the DNA binding site of MYC. c Okanin could significantly inhibit the transcriptional regulatory activity of MYC by luciferase assay. d Okanin significantly inhibited the expressions of GLUT2 and p65, which were the downstream proteins of MYC(n = 3, **P \0.01)
6). YiQi YangYin Decoction Attenuates Nonalcoholic Fatty Liver Disease in Type 2 Diabetes Rats. Evidence-based Complementary and Alternative Medicine, 2021 (PubMed: 34621322)

Application: WB    Species: Rat    Sample: The liver and pancreas tissues

Figure 11 Effect of YQ on insulin secretion-related proteins in pancreas islet. The expressions levels of IRS-2, P-PI3K, PI3K, P-Akt, Akt, and GLUT4 were assessed by western blot analysis. The results are shown as means ± SEM (n = 9–10). ##P < 0.01 compared with the control group; ∗P < 0.05, ∗∗P < 0.01 compared with the model group.
7). Hydrolea Zeylanica Ameliorates Carbohydrate Metabolism Via Modulating GLUTS Expression and Inhibition of Redox-Inflammation in High-Fat Diet Fed. , 2022

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