TRPM7 Antibody - #DF7513
(3)
(5)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
TRPM7 Antibody detects endogenous levels of total TRPM7.
RRID:
AB_2841012
引用形式: Affinity Biosciences Cat# DF7513, RRID:AB_2841012.
引用形式: Affinity Biosciences Cat# DF7513, RRID:AB_2841012.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRPM7; TRPM7_HUMAN;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
- Q96QT4 TRPM7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAGKVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSKQAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
100
Pig
100
Horse
88
Bovine
88
Sheep
88
Dog
88
Xenopus
75
Chicken
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96QT4 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| T523 | Phosphorylation | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| Y776 | Phosphorylation | Uniprot | |
| T778 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| S907 | Phosphorylation | Uniprot | |
| K1159 | Ubiquitination | Uniprot | |
| T1163 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1168 | Acetylation | Uniprot | |
| S1191 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1193 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1230 | Phosphorylation | Uniprot | |
| S1239 | Phosphorylation | Uniprot | |
| K1253 | Ubiquitination | Uniprot | |
| S1255 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1258 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1265 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1269 | Phosphorylation | Uniprot | |
| S1271 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1287 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1358 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1361 | Phosphorylation | Uniprot | |
| S1386 | Phosphorylation | Uniprot | |
| S1387 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1390 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1395 | Phosphorylation | Uniprot | |
| S1396 | Phosphorylation | Uniprot | |
| S1404 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1405 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1407 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1410 | Phosphorylation | Uniprot | |
| S1413 | Phosphorylation | Uniprot | |
| T1417 | Phosphorylation | Uniprot | |
| T1419 | Phosphorylation | Uniprot | |
| T1435 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1446 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1455 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1456 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1463 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1468 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1471 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1473 | Methylation | Uniprot | |
| K1473 | Ubiquitination | Uniprot | |
| S1476 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1477 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1482 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1488 | Phosphorylation | Uniprot | |
| S1493 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1500 | Phosphorylation | Uniprot | |
| S1504 | Phosphorylation | Uniprot | |
| T1505 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1508 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1513 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1527 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1533 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1537 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1542 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1543 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1551 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1567 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1569 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1583 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1598 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1615 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1660 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1685 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1695 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1779 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| T1830 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| K1833 | Ubiquitination | Uniprot | |
| S1851 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
| S1860 | Phosphorylation | Q96QT4 (TRPM7) | Uniprot |
PTMs - Q96QT4 酵素として
| Substrate | Site | Source |
|---|---|---|
| O00418 (EEF2K) | S78 | Uniprot |
| P04083 (ANXA1) | S5 | Uniprot |
| P28289 (TMOD1) | S2 | Uniprot |
| P28289 (TMOD1) | T54 | Uniprot |
| P35579 (MYH9) | T1800 | Uniprot |
| P35579 (MYH9) | S1803 | Uniprot |
| P35579 (MYH9) | S1808 | Uniprot |
| Q96QT4 (TRPM7) | T1163 | Uniprot |
| Q96QT4 (TRPM7) | S1191 | Uniprot |
| Q96QT4 (TRPM7) | S1193 | Uniprot |
| Q96QT4 (TRPM7) | S1255 | Uniprot |
| Q96QT4 (TRPM7) | S1258 | Uniprot |
| Q96QT4 (TRPM7) | T1265 | Uniprot |
| Q96QT4 (TRPM7) | S1271 | Uniprot |
| Q96QT4 (TRPM7) | S1287 | Uniprot |
| Q96QT4 (TRPM7) | S1358 | Uniprot |
| Q96QT4 (TRPM7) | S1387 | Uniprot |
| Q96QT4 (TRPM7) | S1390 | Uniprot |
| Q96QT4 (TRPM7) | S1404 | Uniprot |
| Q96QT4 (TRPM7) | T1405 | Uniprot |
| Q96QT4 (TRPM7) | S1407 | Uniprot |
| Q96QT4 (TRPM7) | T1435 | Uniprot |
| Q96QT4 (TRPM7) | S1446 | Uniprot |
| Q96QT4 (TRPM7) | T1455 | Uniprot |
| Q96QT4 (TRPM7) | S1456 | Uniprot |
| Q96QT4 (TRPM7) | S1463 | Uniprot |
| Q96QT4 (TRPM7) | S1468 | Uniprot |
| Q96QT4 (TRPM7) | T1471 | Uniprot |
| Q96QT4 (TRPM7) | S1476 | Uniprot |
| Q96QT4 (TRPM7) | S1477 | Uniprot |
| Q96QT4 (TRPM7) | T1482 | Uniprot |
| Q96QT4 (TRPM7) | S1493 | Uniprot |
| Q96QT4 (TRPM7) | T1505 | Uniprot |
| Q96QT4 (TRPM7) | T1508 | Uniprot |
| Q96QT4 (TRPM7) | S1513 | Uniprot |
| Q96QT4 (TRPM7) | S1527 | Uniprot |
| Q96QT4 (TRPM7) | S1533 | Uniprot |
| Q96QT4 (TRPM7) | T1537 | Uniprot |
| Q96QT4 (TRPM7) | T1542 | Uniprot |
| Q96QT4 (TRPM7) | S1543 | Uniprot |
| Q96QT4 (TRPM7) | T1551 | Uniprot |
| Q96QT4 (TRPM7) | S1567 | Uniprot |
| Q96QT4 (TRPM7) | S1569 | Uniprot |
| Q96QT4 (TRPM7) | T1583 | Uniprot |
| Q96QT4 (TRPM7) | S1598 | Uniprot |
| Q96QT4 (TRPM7) | S1615 | Uniprot |
| Q96QT4 (TRPM7) | S1660 | Uniprot |
| Q96QT4 (TRPM7) | T1685 | Uniprot |
| Q96QT4 (TRPM7) | S1695 | Uniprot |
| Q96QT4 (TRPM7) | S1779 | Uniprot |
| Q96QT4 (TRPM7) | T1830 | Uniprot |
| Q96QT4 (TRPM7) | S1851 | Uniprot |
| Q96QT4 (TRPM7) | S1860 | Uniprot |
研究背景
機能:
Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
PTMs:
Autophosphorylated.
細胞の位置付け:
Membrane>Multi-pass membrane protein.
サブユニット構造:
Homodimer. Interacts with PLCB1 (By similarity). Forms heterodimers with TRPM6.
タンパク質ファミリー:
In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Digestive system > Mineral absorption.
参考文献
1). Phosphorus magnesium fiber regulates macrophage polarization through TRPM7 to accelerate wound healing. Applied Materials Today, 2023
[IF=8.3]
Application: WB Species: Mouse Sample:
Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance
Application: IF/ICC Species: Mouse Sample:
Fig. 4. PMF promoted wound healing through TRPM7-ERK1/2. (A) The release content and schematic diagram of magnesium ions in the wound site on the 3rd, 7th, and 15th days. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group, n = 3 (B) Western blot analysis of the expression of TRPM7 and phosphorylated ERK1/2 proteins. (C) Quantitative analysis of related protein expression levels in the PMF treatment group compared with the control group. ns: no significance, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3. (D) Immunofluorescent staining images of skin tissue sections on day 7. CD68: pan-macrophage marker (red). TRPM7: (green). Scale bar: 40 μm. (E) Statistical summary of fluorescent expressing of TRPM7/CD68 expressing. ns: no significance
2). Gallium ions promote osteoinduction of human and mouse osteoblasts via the TRPM7/Akt signaling pathway. Molecular Medicine Reports, 2020
(PubMed: 32945378)
[IF=3.4]
Application: WB Species: human Sample: MC3T3 cells
Figure 3. |Effect of TRPM7 siRNAs on TRPM7 protein expression in MC3T3‑E1 and hFOB1.19 cells. (A) Total and (B) membrane TRPM7 protein expression levels in MC3T3 cells following transfection with TRPM7 siRNAs.
3). Novel benzoylurea derivative decreases TRPM7 channel function and inhibits cancer cells migration. Channels (Austin, Tex.), 2024
(PubMed: 39212541)
[IF=3.3]
Application: WB Species: Human Sample: MCF-7 cells
Figure 4. SUD inhibits TRPM7 expression through PI3K/Akt signaling pathway. (a) Effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in MCF-7 cells examined by qPCR. (b) effects of different concentrations SUD on the AKT1 and PI3K mRNA expression in BGC-823 cells examined by qPCR. ***, **** denote significant difference with control group, p
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