LATS1/2 Antibody - #DF7517
(2)
(3)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7517, RRID:AB_2841016.
折りたたみ／展開
h-warts; Large tumor suppressor homolog 1; LATS large tumor suppressor homolog 1; LATS1; LATS1_HUMAN; Serine threonine protein kinase LATS1; Serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts;
免疫原
Expressed in all adult tissues examined except for lung and kidney.
Q9NRM7 LATS2_HUMAN:Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
- O95835 LATS1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPKFGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISKMSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTDVGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGNMEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNGTGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNMELYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNSFNNPLGNRASHSANSQPSATTVTAITPAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESRIQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKMDKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNEWGDPSSCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSDDNEEENVNDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYINSQGSEQQSDEDDQNTGSEIKNRDLVYV
- Q9NRM7 LATS2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRPKTFPATTYSGNSRQRLQEIREGLKQPSKSSVQGLPAGPNSDTSLDAKVLGSKDATRQQQQMRATPKFGPYQKALREIRYSLLPFANESGTSAAAEVNRQMLQELVNAGCDQEMAGRALKQTGSRSIEAALEYISKMGYLDPRNEQIVRVIKQTSPGKGLMPTPVTRRPSFEGTGDSFASYHQLSGTPYEGPSFGADGPTALEEMPRPYVDYLFPGVGPHGPGHQHQHPPKGYGASVEAAGAHFPLQGAHYGRPHLLVPGEPLGYGVQRSPSFQSKTPPETGGYASLPTKGQGGPPGAGLAFPPPAAGLYVPHPHHKQAGPAAHQLHVLGSRSQVFASDSPPQSLLTPSRNSLNVDLYELGSTSVQQWPAATLARRDSLQKPGLEAPPRAHVAFRPDCPVPSRTNSFNSHQPRPGPPGKAEPSLPAPNTVTAVTAAHILHPVKSVRVLRPEPQTAVGPSHPAWVPAPAPAPAPAPAPAAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSEQYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVPVRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQEMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFSSDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPNNKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQTEGCQPVYV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95835/Q9NRM7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T17 | Phosphorylation | Uniprot | |
| Y23 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| R243 | Methylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | Uniprot | |
| R252 | Methylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| R259 | Methylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| T262 | Phosphorylation | Uniprot | |
| K275 | Methylation | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| Y283 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S462 | Phosphorylation | Uniprot | |
| S464 | Phosphorylation | O60285 (NUAK1) , Q9H093 (NUAK2) | Uniprot |
| T490 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S580 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| T612 | Phosphorylation | Uniprot | |
| S613 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T616 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| S674 | Phosphorylation | O95835 (LATS1) | Uniprot |
| K688 | Ubiquitination | Uniprot | |
| K728 | Ubiquitination | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| K751 | Sumoylation | Uniprot | |
| S792 | Phosphorylation | Uniprot | |
| K830 | Sumoylation | Uniprot | |
| S864 | Phosphorylation | Uniprot | |
| S872 | Phosphorylation | Q13188 (STK3) | Uniprot |
| S909 | Phosphorylation | Q13043 (STK4) , Q13188 (STK3) , O95835 (LATS1) | Uniprot |
| T967 | Phosphorylation | Q13188 (STK3) | Uniprot |
| K976 | Ubiquitination | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| K1005 | Ubiquitination | Uniprot | |
| T1012 | Phosphorylation | Q13188 (STK3) | Uniprot |
| K1029 | Ubiquitination | Uniprot | |
| K1046 | Ubiquitination | Uniprot | |
| S1049 | Phosphorylation | O95835 (LATS1) | Uniprot |
| T1060 | Phosphorylation | Q13188 (STK3) | Uniprot |
| Y1076 | Phosphorylation | Uniprot | |
| T1079 | Phosphorylation | Q13043 (STK4) , Q13188 (STK3) | Uniprot |
| S1107 | Phosphorylation | Uniprot | |
| S1111 | Phosphorylation | Uniprot |
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T5 | Phosphorylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| T10 | Phosphorylation | Uniprot | |
| Y11 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | O14965 (AURKA) | Uniprot |
| Y135 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S172 | Phosphorylation | O14757 (CHEK1) , O96017 (CHEK2) | Uniprot |
| S274 | Phosphorylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| T279 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S380 | Phosphorylation | O14965 (AURKA) , O14757 (CHEK1) , O96017 (CHEK2) | Uniprot |
| S408 | Phosphorylation | O96017 (CHEK2) , O14757 (CHEK1) | Uniprot |
| S446 | Phosphorylation | O96017 (CHEK2) , O14757 (CHEK1) | Uniprot |
| S528 | Phosphorylation | Uniprot | |
| Y531 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | Uniprot | |
| K565 | Acetylation | Uniprot | |
| K570 | Acetylation | Uniprot | |
| K571 | Acetylation | Uniprot | |
| T575 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| K620 | Ubiquitination | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| T690 | Phosphorylation | Uniprot | |
| K697 | Ubiquitination | Uniprot | |
| K793 | Ubiquitination | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| S829 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | Q9NRM7 (LATS2) , O14757 (CHEK1) | Uniprot |
| S872 | Phosphorylation | Q13043 (STK4) , Q13188 (STK3) | Uniprot |
| K939 | Ubiquitination | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| K968 | Ubiquitination | Uniprot | |
| K984 | Ubiquitination | Uniprot | |
| T1024 | Phosphorylation | Uniprot | |
| T1026 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| Y1038 | Phosphorylation | Uniprot | |
| T1041 | Phosphorylation | Q13188 (STK3) , Q13043 (STK4) | Uniprot |
| Y1051 | Phosphorylation | Uniprot |
PTMs - O95835/Q9NRM7 酵素として
| Substrate | Site | Source |
|---|---|---|
| O14974 (PPP1R12A) | S445 | Uniprot |
| O95835 (LATS1) | S674 | Uniprot |
| O95835 (LATS1) | S909 | Uniprot |
| O95835 (LATS1) | S1049 | Uniprot |
| P04049 (RAF1) | S259 | Uniprot |
| P46937-2 (YAP1) | S61 | Uniprot |
| P46937-2 (YAP1) | S109 | Uniprot |
| P46937 (YAP1) | S127 | Uniprot |
| P46937-2 (YAP1) | S164 | Uniprot |
| P46937-2 (YAP1) | S381 | Uniprot |
| P46937 (YAP1) | S397 | Uniprot |
| Q02241 (KIF23) | S716 | Uniprot |
| Q02241 (KIF23) | S814 | Uniprot |
| Q8NHZ8 (CDC26) | T7 | Uniprot |
| Q9GZV5 (WWTR1) | S89 | Uniprot |
| Q9NQS7 (INCENP) | S894 | Uniprot |
| Substrate | Site | Source |
|---|---|---|
| O95863 (SNAI1) | T203 | Uniprot |
| P00519-2 (ABL1) | T197 | Uniprot |
| P38936 (CDKN1A) | S146 | Uniprot |
| P46937 (YAP1) | S127 | Uniprot |
| P46937 (YAP1) | S397 | Uniprot |
| P61981 (YWHAG) | S59 | Uniprot |
| Q02241 (KIF23) | S716 | Uniprot |
| Q02241 (KIF23) | S814 | Uniprot |
| Q4VCS5 (AMOT) | S175 | Uniprot |
| Q8NHZ8 (CDC26) | T7 | Uniprot |
| Q9GZV5 (WWTR1) | S89 | Uniprot |
| Q9NQS7 (INCENP) | S894 | Uniprot |
| Q9NRM7 (LATS2) | S835 | Uniprot |
| Q9Y2J4 (AMOTL2) | S159 | Uniprot |
研究背景
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.
Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.
Expressed in all adult tissues examined except for lung and kidney.
Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with ESR1, DCAF1 and DCAF13; probably recruits DCAF1 and DCAF13 to ESR1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation. Interacts with STK3/MST2; this interaction is inhibited in the presence of DLG5. Interacts with SCRIB in the presence of DLG5.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.
Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Nucleus.
Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment.
Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.
Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
研究領域
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
参考文献
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