DDX5 Antibody - #DF7539
| 製品: | DDX5 Antibody |
| カタログ: | DF7539 |
| タンパク質の説明: | Rabbit polyclonal antibody to DDX5 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Dog |
| 分子量: | 69 kDa; 69kD(Calculated). |
| ユニプロット: | P17844 |
| RRID: | AB_2841038 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7539, RRID:AB_2841038.
折りたたみ／展開
ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5_HUMAN; DEAD (Asp Glu Ala Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD); G17P1; HELR; HLR1; HUMP68; P68; p68 RNA helicase; Probable ATP dependent RNA helicase DDX5; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68;
免疫原
- P17844 DDX5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P17844 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| R10 | Methylation | Uniprot | |
| R12 | Methylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| R20 | Methylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| R25 | Methylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| K32 | Acetylation | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K40 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K43 | Acetylation | Uniprot | |
| K44 | Acetylation | Uniprot | |
| K45 | Acetylation | Uniprot | |
| K45 | Sumoylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K53 | Acetylation | Uniprot | |
| K53 | Sumoylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Methylation | Uniprot | |
| K56 | Sumoylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y59 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| K80 | Acetylation | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| T83 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| Y97 | Phosphorylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K197 | Methylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S198 | Phosphorylation | P49137 (MAPKAPK2) | Uniprot |
| T199 | Phosphorylation | Uniprot | |
| C200 | S-Nitrosylation | Uniprot | |
| Y202 | Phosphorylation | Uniprot | |
| K207 | Sumoylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| C234 | S-Nitrosylation | Uniprot | |
| K236 | Acetylation | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| R263 | Methylation | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| R273 | Methylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| K340 | Acetylation | Uniprot | |
| K340 | Methylation | Uniprot | |
| K340 | Ubiquitination | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| T344 | Phosphorylation | Uniprot | |
| K351 | Acetylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| K388 | Acetylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| K391 | Sumoylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| R403 | Methylation | Uniprot | |
| K411 | Sumoylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| Y416 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| Y425 | Phosphorylation | Uniprot | |
| K437 | Sumoylation | Uniprot | |
| K437 | Ubiquitination | Uniprot | |
| T438 | Phosphorylation | Uniprot | |
| T440 | Phosphorylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot | |
| T446 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| K451 | Ubiquitination | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| K470 | Acetylation | Uniprot | |
| K470 | Sumoylation | Uniprot | |
| K470 | Ubiquitination | Uniprot | |
| R478 | Methylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| R484 | Methylation | Uniprot | |
| R486 | Methylation | Uniprot | |
| K490 | Methylation | Uniprot | |
| S498 | Phosphorylation | Uniprot | |
| K501 | Acetylation | Uniprot | |
| R502 | Methylation | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| R509 | Methylation | Uniprot | |
| Y514 | Phosphorylation | Uniprot | |
| R516 | Methylation | Uniprot | |
| Y518 | Phosphorylation | Uniprot | |
| S519 | Phosphorylation | Uniprot | |
| S520 | Phosphorylation | Uniprot | |
| K523 | Sumoylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| Y535 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| T564 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| Y593 | Phosphorylation | P00519 (ABL1) | Uniprot |
| Y595 | Phosphorylation | Uniprot |
研究背景
Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.
Arg-502 is dimethylated, probably to asymmetric dimethylarginine.
Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination.
Polyubiquitinated, leading to proteasomal degradation.
Nucleus>Nucleolus.
Identified in the spliceosome C complex. Interacts with RBM4; the interaction occurs in an RNA-independent manner. Interacts with AGO1 and AGO2. Interacts with ESR1, AR, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interacts with DDX17. Interacts with BRDT. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active). Interacts with DHX36; this interaction occurs in a RNA-dependent manner. Interacts with NUPR1 (By similarity). Interacts with ERCC6.
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.
研究領域
· Genetic Information Processing > Transcription > Spliceosome.
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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