ASC2 Antibody - #DF7540

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製品: ASC2 Antibody
カタログ: DF7540
タンパク質の説明: Rabbit polyclonal antibody to ASC2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 10 kDa; 10kD(Calculated).
ユニプロット: Q8WXC3
RRID: AB_2841039

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
ASC2 Antibody detects endogenous levels of total ASC2.
RRID:
AB_2841039
引用形式: Affinity Biosciences Cat# DF7540, RRID:AB_2841039.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

PAAD domain only protein; PAAD-only protein 1; POP1; PYC1; PYD (pyrin domain) containing 1; PYDC1; PYDC1_HUMAN; Pyrin domain containing 1; Pyrin domain-containing protein 1; Pyrin only protein 1; Pyrin-only protein 1;

免疫原

免疫原:
Uniprot:

Q8WXC3(PYDC1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PYDC1(260434)
発現特異性:
Q8WXC3 PYDC1_HUMAN:

Predominantly expressed in monocytes, macrophages and granulocytes.

タンパク質配列:
MGTKREAILKVLENLTPEELKKFKMKLGTVPLREGFERIPRGALGQLDIVDLTDKLVASYYEDYAAELVVAVLRDMRMLEEAARLQRAA

研究背景

機能:

Associates with PYCARD/ASC and modulates its ability to collaborate with MEFV/pyrin and NLRP3/cryopyrin in NF-kappa-B and pro-caspase-1 activation. Suppresses kinase activity of NF-kappa-B inhibitor kinase (IKK) complex, expression of NF-kappa-B inducible genes and inhibits NF-kappa-B activation by cytokines and LPS.

PTMs:

Phosphorylated.

細胞の位置付け:

Cytoplasm.
Note: Recruited to specks formed by PYCARD within the cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Predominantly expressed in monocytes, macrophages and granulocytes.

サブユニット構造:

Interacts with PYCARD/ASC (via pyrin domain).

研究領域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

参考文献

1). lncRNA‑MALAT1 promotes high glucose‑induced H9C2 cardiomyocyte pyroptosis by downregulating miR‑141‑3p expression. Molecular Medicine Reports, 2021 (PubMed: 33576445) [IF=3.4]

Application: WB    Species: Human    Sample: H9C2 cells

Figure 3. Effects of MALAT1 on pyroptosis-associated protein expression levels. ASC, GSDMD-N, caspase-1, NLRP3 and GSDMD protein expression levels were measured via western blotting. *P<0.05 and **P<0.01 vs. NG; #P<0.05 and ##P<0.01 vs. HG; ^P<0.05 and ^^P<0.01 vs. NC1; %P<0.05 vs. NC2. MALAT1, metastasis-associated lung adenocarcinoma transcript-1; ASC, apoptosis-associated speck-like protein; GSDMD, gasdermin D; NLRP3, nucleotide oligomerization domain-like receptor protein 3; NG, normal glucose; HG, high glucose; NC, negative control; NC1, MALAT1 overexpression NC group; NC2, MALAT1 knockdown NC group; si, small interfering RNA.

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