RAB7 Antibody - #DF7546
製品: | RAB7 Antibody |
カタログ: | DF7546 |
タンパク質の説明: | Rabbit polyclonal antibody to RAB7 |
アプリケーション: | WB IHC |
反応性: | Human, Mouse, Rat |
予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 23 kDa; 23kD(Calculated). |
ユニプロット: | P51149 |
RRID: | AB_2841045 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7546, RRID:AB_2841045.
折りたたみ/展開
CMT2B; PRO2706; PSN; RAB7, member RAS oncogene family; RAB7A; RAB7A, member RAS oncogene family; RAB7A_HUMAN; Ras associated protein RAB7; Ras related protein Rab7; Ras related protein Rab7a; Ras-related protein Rab-7a;
免疫原
- P51149 RAB7A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51149 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T2 | Acetylation | Uniprot | |
S17 | Phosphorylation | Uniprot | |
K21 | Ubiquitination | Uniprot | |
Y28 | Phosphorylation | Uniprot | |
K31 | Ubiquitination | Uniprot | |
K32 | Ubiquitination | Uniprot | |
Y37 | Phosphorylation | Uniprot | |
K38 | Ubiquitination | Uniprot | |
T40 | Phosphorylation | Uniprot | |
K48 | Ubiquitination | Uniprot | |
R55 | Methylation | Uniprot | |
S72 | Phosphorylation | Uniprot | |
C83 | S-Nitrosylation | Uniprot | |
C84 | S-Nitrosylation | Uniprot | |
K97 | Ubiquitination | Uniprot | |
S101 | Phosphorylation | Uniprot | |
S111 | Phosphorylation | Uniprot | |
K126 | Ubiquitination | Uniprot | |
R132 | Methylation | Uniprot | |
K137 | Ubiquitination | Uniprot | |
K146 | Ubiquitination | Uniprot | |
K157 | Ubiquitination | Uniprot | |
T168 | Phosphorylation | Uniprot | |
K175 | Ubiquitination | Uniprot | |
T178 | Phosphorylation | Uniprot | |
Y183 | Phosphorylation | Uniprot | |
K191 | Ubiquitination | Uniprot | |
K194 | Ubiquitination | Uniprot | |
S201 | Phosphorylation | Uniprot | |
S204 | Phosphorylation | Uniprot | |
S206 | Phosphorylation | Uniprot |
研究背景
Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan.
Cytoplasmic vesicle>Phagosome membrane>Peripheral membrane protein>Cytoplasmic side. Late endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lysosome membrane>Peripheral membrane protein>Cytoplasmic side. Melanosome membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein>Cytoplasmic side. Lipid droplet. Endosome membrane.
Note: Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255211). Lipid droplet localization is increased upon ADRB2 stimulation (By similarity).
Widely expressed; high expression found in skeletal muscle.
The GTP-bound form interacts with RAC1 (By similarity). Interacts with NTRK1/TRKA (By similarity). Interacts with C9orf72 (By similarity). Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity). Interacts with RILP. Interacts with PSMA7. Interacts with RNF115. Interacts with FYCO1. Interacts with the PIK3C3/VPS34-PIK3R4 complex. The GTP-bound form interacts with OSBPL1A. Interacts with CLN3. Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4). Interacts with CLN5. Interacts with PLEKHM1 (via N- and C-terminus) (By similarity). Interacts with RUFY4.
Belongs to the small GTPase superfamily. Rab family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
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