Aggrecan Antibody - #DF7561

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製品: Aggrecan Antibody
カタログ: DF7561
タンパク質の説明: Rabbit polyclonal antibody to Aggrecan
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 70,150,250 kDa; 261kD(Calculated).
ユニプロット: P16112
RRID: AB_2841055

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Aggrecan Antibody detects endogenous levels of total Aggrecan.
RRID:
AB_2841055
引用形式: Affinity Biosciences Cat# DF7561, RRID:AB_2841055.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ACAN; AGC 1; AGC1; AGCAN; Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); Aggrecan 1; Aggrecan core protein; Aggrecan proteoglycan; Aggrecan structural proteoglycan of cartilage; Aggrecan1; ATEGQV; Cartilage specific proteoglycan core protein; Chondroitin sulfate proteoglycan 1; Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122; Chondroitin sulfate proteoglycan core protein 1; CSPG 1; CSPG1; CSPGCP; JSCATE; Large aggregating proteoglycan; mcspg; mgsk16; MSK 16; MSK16; SEDK;

免疫原

免疫原:

A synthesized peptide derived from human Aggrecan, corresponding to a region within N-terminal amino acids.

Uniprot:

P16112(PGCA_HUMAN) >>Visit HPA database.

遺伝子(ID):
ACAN(176)
発現特異性:
P16112 PGCA_HUMAN:

Restricted to cartilages.

タンパク質配列:
MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAAAPARSCAEEPCGAGTCKETEGHVICLCPPGYTGEHCNIDQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDPTTYKRRLQKRSSRHPRRSRPSTAH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
78
Chicken
67
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

PTMs:

Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Restricted to cartilages.

タンパク質ファミリー:

Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan, while another globular region, G3, makes up the C-terminus. G1 contains Link domains and thus consists of three disulfide-bonded loop structures designated as the A, B, B' motifs. G2 is similar to G1. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains lie between G2 and G3.

Belongs to the aggrecan/versican proteoglycan family.

参考文献

1). Hydrogen Ion Capturing Hydrogel Microspheres for Reversing Inflammaging. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 37699155) [IF=27.4]
2). An anti-inflammatory and neuroprotective biomimetic nanoplatform for repairing spinal cord injury. Bioactive Materials, 2022 (PubMed: 35845318) [IF=18.9]
3). Bioactive Patch for Rotator Cuff Repairing via Enhancing Tendon-to-Bone Healing: A Large Animal Study and Short-Term Outcome of a Clinical Trial. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024 (PubMed: 38922803) [IF=15.1]

Application: IF/ICC    Species: human    Sample: BMSCs

Figure 6 Chondrogenic evaluation of UC extracts. A–C) Immunofluorescence staining for Aggrecan, SOX9 and COL-II in BMSCs under different induction conditions. D,E) Positive rate and mean fluorescence intensity of Immunofluorescence. F,G) Western-blot of Aggrecan, SOX9 and COL-II in BMSCs under different induction conditions. H) Chondrogenic pellet evaluation by staining under different induction conditions. I) Positive rate of immunohistochemistry of pellet staining. The results are presented as means ± SD. (*P < 0.05 compared with control.).

Application: WB    Species: human    Sample: BMSCs

Figure 6 Chondrogenic evaluation of UC extracts. A–C) Immunofluorescence staining for Aggrecan, SOX9 and COL-II in BMSCs under different induction conditions. D,E) Positive rate and mean fluorescence intensity of Immunofluorescence. F,G) Western-blot of Aggrecan, SOX9 and COL-II in BMSCs under different induction conditions. H) Chondrogenic pellet evaluation by staining under different induction conditions. I) Positive rate of immunohistochemistry of pellet staining. The results are presented as means ± SD. (*P < 0.05 compared with control.).
4). Sustained therapeutic effects of self-assembled hyaluronic acid nanoparticles loaded with α-Ketoglutarate in various osteoarthritis stages. Biomaterials, 2024 (PubMed: 39326362) [IF=12.8]

Application: IHC    Species: Mouse    Sample:

Fig. 9. αKG protects articular cartilage by modulating cartilage matrix homeostasis. Immunohistochemistry staining of Col2a1, Acan, MMP13, and Adamts4 in the knee joint cartilage of advanced OA mice under different treatments.
5). The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3. Redox biology, 2023 (PubMed: 37099926) [IF=10.7]
6). Loss of DDRGK1 impairs IRE1α UFMylation in spondyloepiphyseal dysplasia. International Journal of Biological Sciences, 2023 (PubMed: 37781516) [IF=8.2]

Application: IF/ICC    Species: Mouse    Sample:

Figure 6 Loss of DDRGK1 or the K268R mutation led to IRE1α degradation and ER stress aggravation in the growth plate cells of mice in vivo. (A) Immunofluorescence analysis of SOX9, Aggrecan, IRE1α and CHOP expression in the lower limbs of the WT and cKO mice shown in Figure ​Figure1A;1A; the growth plate is the focus. (B) Quantification of the integrated optical density (IOD)/DAPI stain intensity levels of SOX9, Aggrecan, IRE1α and CHOP in the images shown in panel (A). (C) Alcian blue staining of primary chondrocytes from WT and mutant mice 9 days after high-density culturing in chondrogenesis medium. (D) Quantification of the relative IOD/area ratio in the Alcian blue-stained cells shown in panel (C). (E) Safranin O-Fast Green staining of the lower limbs in WT and mutant mice; the growth plate is the focus. (F) Quantification of the length of the hypertrophy zone (HZ) shown in panel (E). (G) TUNEL immunofluorescence staining of the lower limbs of the WT and cKO mice shown in Figure ​Figure1A1A with focus on the growth plate. (H) Quantification of the percentage of TUNEL-positive cells in the growth plate shown in panel (G). (I) Reverse transcription-quantitative PCR analysis performed to determine the relative mRNA expression levels of ATF4, BIP and CHOP in primary WT and Mutant mouse chondrocytes treated with or without thapsigargin (Tg) for 24 h; β-actin was the internal reference. (J) Western blot analysis of DDRGK1, IRE1α, BIP, ATF4 and CHOP of primary WT and mutant mouse chondrocytes treated with or without Tg for 24 h; β-actin was the loading control. (K) Immunofluorescence analysis of IRE1α, XBP-1s and CHOP expression in the lower limbs of the WT and mutant mice shown in panel (E); the growth plate is the focus. (L) Quantification of the IOD/DAPI stain intensity levels of IRE1α, XBP-1s and CHOP in the chondrocytes shown in panel (K). (M) Western blot analysis of cleaved and full-length PARP and cleaved Caspase 3 in primary in WT and mutant mouse chondrocytes treated with or without Tg for 24 h; β-actin was the loading control. All data are presented as the mean ± SD from three or more experiments.
7). Vitamin K2 ameliorates osteoarthritis by suppressing ferroptosis and extracellular matrix degradation through activation GPX4's dual functions. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38759289) [IF=6.9]

Application: WB    Species: Rat    Sample:

Fig. 8. GPX4 inhibitor RSL3 substantially attenuates VK2 protection of chondrocytes. (A-I) WB results for GPX4, NFκB, pMAPK/MAPK, Aggrecan, CollagenⅡ, SOX9, MMP3, MMP13 and Tubulin; (J) Quantitive analysis of relative Glutathione Peroxidase Activity; (K-L) GSH contents and ratio of GSH/GSSG; (M) Quantitative results of MDA content assay.
8). Combination of curcumin and catalase protects against chondrocyte injury and knee osteoarthritis progression by suppressing oxidative stress. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 37879214) [IF=6.9]
9). TMF inhibits extracellular matrix degradation by regulating the C/EBPβ/ADAMTS5 signaling pathway in osteoarthritis. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38554527) [IF=6.9]

Application: IHC    Species: Rat    Sample:

Fig. 1. The effect of TMF on the articular cartilage of OA rats (n = 6) was investigated. (A) The articular cartilage was stained with H/E. IHC assays were used to detect the altered expression of Aggrecan (B–C), ADAMTS5 (D–E), and Caspase3 (F–G) in the cartilage.
10). Disc regeneration by injectable fucoidan-methacrylated dextran hydrogels through mechanical transduction and macrophage immunomodulation. Journal of tissue engineering, 2023 (PubMed: 37427012) [IF=6.7]
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