HES1 Antibody - #DF7569

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製品: HES1 Antibody
カタログ: DF7569
タンパク質の説明: Rabbit polyclonal antibody to HES1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 30 kDa; 30kD(Calculated).
ユニプロット: Q14469
RRID: AB_2841062

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
HES1 Antibody detects endogenous levels of total HES1.
RRID:
AB_2841062
引用形式: Affinity Biosciences Cat# DF7569, RRID:AB_2841062.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

bHLHb39; C-HAIRY1; c-hairy1A; Class B basic helix-loop-helix protein 39; FLJ20408; Hairy and enhancer of split 1 (Drosophila); Hairy and enhancer of split 1; Hairy homolog (Drosophila); Hairy homolog; Hairy like; Hairy, Drosophila, homolog of; Hairy-like protein; Hairy/enhancer of split, Drosophila, homolog of, 1; HAIRY1; HES-1; hes1; Hes1 hairy and enhancer of split 1 (Drosophila); HES1_HUMAN; HHL; HL; HRY; MGC129109; OTTHUMP00000209031; RHL; Transcription factor HES-1;

免疫原

免疫原:
Uniprot:

Q14469(HES1_HUMAN) >>Visit HPA database.

遺伝子(ID):
HES1(3280)
タンパク質配列:
MPADIMEKNSSSPVAATPASVNTTPDKPKTASEHRKSSKPIMEKRRRARINESLSQLKTLILDALKKDSSRHSKLEKADILEMTVKHLRNLQRAQMTAALSTDPSVLGKYRAGFSECMNEVTRFLSTCEGVNTEVRTRLLGHLANCMTQINAMTYPGQPHPALQAPPPPPPGPGGPQHAPFAPPPPLVPIPGGAAPPPGGAPCKLGSQAGEAAKVFGGFQVVPAPDGQFAFLIPNGAFAHSGPVIPVYTSNSGTSVGPNAVSPSSGPSLTADSMWRPWRN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
92
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14469 基板として

Site PTM Type Enzyme
S10 Phosphorylation
S11 Phosphorylation
S12 Phosphorylation
T24 Phosphorylation
S32 Phosphorylation
S37 Phosphorylation P17252 (PRKCA)
S38 Phosphorylation P17252 (PRKCA)
S55 Phosphorylation
T59 Phosphorylation
K77 Ubiquitination
K86 Ubiquitination
T97 Phosphorylation
K109 Ubiquitination

研究背景

機能:

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family. Interacts (via WPRW motif) with TLE1, and more weakly with TLE2. Interacts with HES6 (By similarity). Interacts with SIRT1. Interacts with an FA complex, composed of FANCA, FANCF, FANCG and FANCL, but not of FANCC, nor FANCE.

タンパク質ファミリー:

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).

The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.

The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.

研究領域

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

参考文献

1). Notch1 contributes to TNF-α-induced proliferation and migration of airway smooth muscle cells through regulation of the Hes1/PTEN axis. International Immunopharmacology, 2020 (PubMed: 32871474) [IF=5.6]

Application: WB    Species: mouse    Sample: ASM cells

Fig. 5.| Notch1 knockdown decreased Hes1 and increased PTEN expression in TNF-α-induced ASM cells. ASM cells were transfected with NC or Notch1 siRNA for 48 h and then stimulated with TNF-α for 24 h. (D, E) Protein expression of Hes1 was examined by western blot.
2). Neural stem cell-derived exosomes regulate neural stem cell differentiation through miR-9-Hes1 axis. Frontiers in Cell and Developmental Biology, 2021 (PubMed: 34055767) [IF=5.5]

Application: WB    Species: mouse    Sample: NSCs

FIGURE 7 | Hes1 expression is negatively regulated by miR-9 in NSCs.(C) Representative western blotting results showing the expression of Hes1 and β-actin proteins in either agomiR-C- or agomiR-9-transfected NSCs.
3). TMT-based quantitative proteomics analysis reveals the role of Notch signaling in FAdV-4-infected LMH cell. Frontiers in Microbiology, 2022 (PubMed: 36187945) [IF=5.2]
4). The regulatory roles of VEGF-Notch signaling pathway on aplastic anemia with kidney deficiency and blood stasis. JOURNAL OF CELLULAR BIOCHEMISTRY, 2019 (PubMed: 30230583) [IF=4.0]

Application: WB    Species: human    Sample: HUVECs

FIGURE 5| The expression of VEGF‐Notch signaling pathway related proteins (VEGF, VEGFR, Notch‐1, Jagged1, Delta‐like1, and hes1)in HUVECs cocultured with VEGF/Notch shRNA transfected BMSCs (n = 3). *P < 0.05 vs HUVECs (normal control); #P < 0.05 vs BMSC + NC shRNA + HUVEC. BMSC, bone marrow mesenchymal stem cell; HUVEC, human umbilical vein endothelial cell; NC, normal control; shRNA, short hairpin RNA; VEGF, vascular endothelial growth factor; VEGFR, VEGF receptor
5). Minocycline Attenuates Sevoflurane-Induced Postoperative Cognitive Dysfunction in Aged Mice by Suppressing Hippocampal Apoptosis and the Notch Signaling Pathway-Mediated Neuroinflammation. Brain Sciences, 2023 (PubMed: 36979321) [IF=3.3]

Application: WB    Species: Mouse    Sample: hippocampal tissue

Figure 6 Minocycline alleviates sevoflurane-induced neuroinflammation via Notch signaling suppression: (A) Cell immunofluorescence was performed to assess Notch signaling-associated proteins in BV2 cells of each group. Scale bar, 20 μm; (B–G) Immunoblot detection of Notch1, cleaved Notch1, and Hes1 in BV2 cells and hippocampal tissue specimens. β-actin was utilized for normalizing data quantitated by densitometry with ImageJ. Data are mean ± SEM.
6). Astragalus Polysaccharide Promotes Neuronal Injury Repair via the Notch1/NFκB Signaling Axis in the Ventroposterior Thalamic Nucleus in Rats with Focal Cerebral Ischemia. Journal of integrative neuroscience, 2024 (PubMed: 38419443) [IF=1.8]

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