LEF1 Antibody - #DF7570

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製品: LEF1 Antibody
カタログ: DF7570
タンパク質の説明: Rabbit polyclonal antibody to LEF1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 55 kDa; 44kD(Calculated).
ユニプロット: Q9UJU2
RRID: AB_2841063

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
LEF1 Antibody detects endogenous levels of total LEF1.
RRID:
AB_2841063
引用形式: Affinity Biosciences Cat# DF7570, RRID:AB_2841063.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DKFZp586H0919; FLJ46390; LEF 1; LEF-1; Lef1; LEF1_HUMAN; Lymphoid enhancer binding factor 1; Lymphoid enhancer-binding factor 1; T cell specific transcription factor 1 alpha; T cell-specific transcription factor 1-alpha; TCF 1 alpha; TCF1 alpha; TCF1-alpha; TCF10; TCF1alpha; TCF7L3; Transcription factor T cell specific 1 alpha;

免疫原

免疫原:
Uniprot:

Q9UJU2(LEF1_HUMAN) >>Visit HPA database.

遺伝子(ID):
LEF1(51176)
発現特異性:
Q9UJU2 LEF1_HUMAN:

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

タンパク質配列:
MPQLSGGGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNNDPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPDIPTFYPLSPGGVGQITPPLGWQGQPVYPITGGFRQPYPSSLSVDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQESASGTGPRMTAAYI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UJU2 基板として

Site PTM Type Enzyme
S5 Phosphorylation
K36 Ubiquitination
S42 Phosphorylation P68400 (CSNK2A1)
K54 Ubiquitination
S61 Phosphorylation P68400 (CSNK2A1)
S79 Phosphorylation
Y83 Phosphorylation
K86 Acetylation
K86 Ubiquitination
K95 Acetylation
K95 Ubiquitination
Y102 Phosphorylation
Y114 Phosphorylation
S132 Phosphorylation Q9UBE8 (NLK)
T155 Phosphorylation Q9UBE8 (NLK)
T159 Phosphorylation
Y160 Phosphorylation
S161 Phosphorylation
S166 Phosphorylation Q9UBE8 (NLK)
T195 Phosphorylation
Y197 Phosphorylation
S200 Phosphorylation Q9UBE8 (NLK)
T208 Phosphorylation
R226 Methylation
S238 Phosphorylation
S240 Phosphorylation
R241 Methylation
T265 Phosphorylation Q9UBE8 (NLK)
K283 Ubiquitination
K295 Ubiquitination
K301 Ubiquitination
K311 Ubiquitination
K324 Ubiquitination
K347 Ubiquitination
Y348 Phosphorylation
Y349 Phosphorylation
Y363 Phosphorylation
S367 Phosphorylation
K382 Ubiquitination
S386 Phosphorylation
T390 Phosphorylation
R393 Methylation

研究背景

機能:

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

PTMs:

Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway.

細胞の位置付け:

Nucleus.
Note: Found in nuclear bodies upon PIASG binding.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines.

サブユニット構造:

Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds ALYREF/THOC4, MDFI and MDFIC. Interacts with NLK.

タンパク質ファミリー:

Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.

Belongs to the TCF/LEF family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

· Organismal Systems > Endocrine system > Melanogenesis.

参考文献

1). PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia. Journal of Translational Medicine, 2021 (PubMed: 34001134) [IF=7.4]

Application: WB    Species: human    Sample:

Fig. 7| High expression of PRICKEL1 accompanied with core PCP pathway components and the migration and invasion components up-regulation in AML patients. a The amounts of the indicated PCP proteins were analyzed by Western blotting in AML patients (n=5) and normal controls(n=3).
2). Liraglutide Regulates Bone Destruction and Exhibits Anti-inflammatory Effects in Periodontitis In vitro and In vivo. JOURNAL OF DENTISTRY, 2020 (PubMed: 32119967) [IF=4.4]

Application: WB    Species: human    Sample: hPDLCs

Fig. 4.| LIRA enhanced the osteogenic differentiation of hPDLCs in an inflammatory microenvironment via partial inhibition of the Wnt/β-catenin signaling pathway.(A) Expression levels of nuclear β-catenin (N-β-cat) and nuclear LEF1 (N-LEF1) in the four groups. *P < 0.05 vs. controls, **P < 0.01 vs. controls, #P < 0.05 vs. The LPS group, ##P < 0.01 vs. the LPS group.
3). NOX4 stimulates ANF secretion via activation of the Sirt1/Nrf2/ATF3/4 axis in hypoxic beating rat atria. Molecular Medicine Reports, 2022 (PubMed: 35029280) [IF=3.4]

Application: WB    Species: rat    Sample: atria

Figure 5.| Effect of nuclear factor erythroid‑2‑related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria.(A)Representative western blots.

Application: IF/ICC    Species: rat    Sample: atria

Figure 5.| Effect of nuclear factor erythroid‑2‑related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria. (C) LEF1 protein expression was detected using immunofluorescence staining (scale bar, 100 µm). Data are presented as the mean ± SE. n=5. ***P<0.001 vs. control; ###P<0.001 vs. hypoxia. TCF, T‑cell factor; LEF1, lymphoid enhancer factor 1; cont, control; hy, hypoxia; ML, ML385.

Application: WB    Species: Rat    Sample:

Figure 5. Effect of nuclear factor erythroid-2-related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria. (A) Representative western blots. (B) Quantification of protein expression levels. (C) LEF1 protein expression was detected using immunofluorescence staining (scale bar, 100 µm). Data are presented as the mean ± SE. n=5. ***P<0.001 vs. control; ###P<0.001 vs. hypoxia. TCF, T-cell factor; LEF1, lymphoid enhancer factor 1; cont, control; hy, hypoxia; ML, ML385.

Application: IF/ICC    Species: Rat    Sample:

Figure 5. Effect of nuclear factor erythroid-2-related factor 2 inhibitor on TCF3, TCF4 and LEF1 protein expression induced by hypoxia in beating rat atria. (A) Representative western blots. (B) Quantification of protein expression levels. (C) LEF1 protein expression was detected using immunofluorescence staining (scale bar, 100 µm). Data are presented as the mean ± SE. n=5. ***P<0.001 vs. control; ###P<0.001 vs. hypoxia. TCF, T-cell factor; LEF1, lymphoid enhancer factor 1; cont, control; hy, hypoxia; ML, ML385.
4). Src-IL-18 signaling regulates the secretion of atrial natriuretic factor in hypoxic beating rat atria. Kardiologia Polska, 2021 (PubMed: 34176112) [IF=3.3]

Application: WB    Species: rat    Sample: atria

Figure 4. | A, B. Effect of IL-18 on the protein levels of ATF3, TCF3, TCF4 and LEF1.

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