製品: Prohibitin Antibody
カタログ: DF7600
タンパク質の説明: Rabbit polyclonal antibody to Prohibitin
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 30 kDa; 30kD(Calculated).
ユニプロット: P35232
RRID: AB_2841091

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
Prohibitin Antibody detects endogenous levels of total Prohibitin.
RRID:
AB_2841091
引用形式: Affinity Biosciences Cat# DF7600, RRID:AB_2841091.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Epididymis luminal protein 215; Epididymis secretory sperm binding protein Li 54e; HEL 215; HEL S 54e; PHB; PHB_HUMAN; PHB1; Prohibitin;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P35232 PHB_HUMAN:

Widely expressed in different tissues.

タンパク質配列:
MAAKVFESIGKFGLALAVAGGVVNSALYNVDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSKDLQNVNITLRILFRPVASQLPRIFTSIGEDYDERVLPSITTEILKSVVARFDAGELITQRELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKAEQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAGQSVLLQLPQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35232 基板として

Site PTM Type Enzyme
A2 Acetylation
K4 Acetylation
K4 Methylation
K4 Ubiquitination
K11 Methylation
R41 Methylation
S82 Phosphorylation
K83 Ubiquitination
T91 Phosphorylation
R93 Methylation
S101 Phosphorylation
T108 Phosphorylation
S109 Phosphorylation
Y114 Phosphorylation P06213 (INSR)
S121 O-Glycosylation
T123 Phosphorylation
K128 Acetylation
K128 Ubiquitination
S151 Phosphorylation
K177 Ubiquitination
K186 Ubiquitination
K202 Acetylation
K202 Ubiquitination
K207 Acetylation
K208 Ubiquitination
S213 Phosphorylation
S218 Phosphorylation
K219 Ubiquitination
K240 Ubiquitination
Y249 Phosphorylation
S252 Phosphorylation
S254 Phosphorylation
T258 O-Glycosylation
T258 Phosphorylation P31749 (AKT1)
Y259 Phosphorylation
S265 Phosphorylation

研究背景

機能:

Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

細胞の位置付け:

Mitochondrion inner membrane. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed in different tissues.

サブユニット構造:

Interacts with PHB2. Interacts with STOML2.

タンパク質ファミリー:

Belongs to the prohibitin family.

Restrictive clause

 

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.