SREBP2 Antibody - #DF7601

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製品: SREBP2 Antibody
カタログ: DF7601
タンパク質の説明: Rabbit polyclonal antibody to SREBP2
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 124kDa; 124kD(Calculated).
ユニプロット: Q12772
RRID: AB_2841092

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
SREBP2 Antibody detects endogenous levels of total SREBP2.
RRID:
AB_2841092
引用形式: Affinity Biosciences Cat# DF7601, RRID:AB_2841092.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AI608257; bHLHd2; Class D basic helix-loop-helix protein 2; OTTHUMP00000028740; Processed sterol regulatory element-binding protein 2; SRBP2_HUMAN; SREBF 2; Srebf2; SREBF2 protein; SREBP 2; SREBP-2; SREBP2; SREBP2gc; sterol regulatory element binding factor 2; Sterol regulatory element binding protein 2; Sterol regulatory element binding transcription factor 2; Sterol regulatory element-binding transcription factor 2;

免疫原

免疫原:
Uniprot:

Q12772(SRBP2_HUMAN) >>Visit HPA database.

遺伝子(ID):
SREBF2(6721)
発現特異性:
Q12772 SRBP2_HUMAN:

Ubiquitously expressed in adult and fetal tissues.

タンパク質配列:
MDDSGELGGLETMETLTELGDELTLGDIDEMLQFVSNQVGEFPDLFSEQLCSSFPGSGGSGSSSGSSGSSSSSSNGRGSSSGAVDPSVQRSFTQVTLPSFSPSAASPQAPTLQVKVSPTSVPTTPRATPILQPRPQPQPQPQTQLQQQTVMITPTFSTTPQTRIIQQPLIYQNAATSFQVLQPQVQSLVTSSQVQPVTIQQQVQTVQAQRVLTQTANGTLQTLAPATVQTVAAPQVQQVPVLVQPQIIKTDSLVLTTLKTDGSPVMAAVQNPALTALTTPIQTAALQVPTLVGSSGTILTTMPVMMGQEKVPIKQVPGGVKQLEPPKEGERRTTHNIIEKRYRSSINDKIIELKDLVMGTDAKMHKSGVLRKAIDYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLKIEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDRSRILLCVLTFLCLSFNPLTSLLQWGGAHDSDQHPHSGSGRSVLSFESGSGGWFDWMMPTLLLWLVNGVIVLSVFVKLLVHGEPVIRPHSRSSVTFWRHRKQADLDLARGDFAAAAGNLQTCLAVLGRALPTSRLDLACSLSWNVIRYSLQKLRLVRWLLKKVFQCRRATPATEAGFEDEAKTSARDAALAYHRLHQLHITGKLPAGSACSDVHMALCAVNLAECAEEKIPPSTLVEIHLTAAMGLKTRCGGKLGFLASYFLSRAQSLCGPEHSAVPDSLRWLCHPLGQKFFMERSWSVKSAAKESLYCAQRNPADPIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALEVTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLLSLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCNDCQQMIVKLGGGTAIAAS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q12772 基板として

Site PTM Type Enzyme
S79 Phosphorylation
S80 Phosphorylation
S81 Phosphorylation
S101 Phosphorylation
S106 Phosphorylation
K115 Ubiquitination
S117 Phosphorylation
S120 Phosphorylation
T124 Phosphorylation
T153 Phosphorylation
T155 Phosphorylation
Y171 Phosphorylation
K249 Sumoylation
S252 Phosphorylation
K314 Ubiquitination
K321 Acetylation
K340 Ubiquitination
K349 Ubiquitination
K354 Ubiquitination
K363 Acetylation
K363 Ubiquitination
K366 Ubiquitination
K372 Ubiquitination
K378 Ubiquitination
K386 Ubiquitination
K395 Ubiquitination
K400 Ubiquitination
K405 Ubiquitination
K420 Sumoylation
S432 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S455 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
K464 Sumoylation
K464 Ubiquitination
S469 Phosphorylation
K579 Ubiquitination
K630 Acetylation
K640 Ubiquitination
K660 Ubiquitination
K731 Ubiquitination
S745 Phosphorylation
K768 Ubiquitination
K778 Ubiquitination
K782 Ubiquitination
K805 Ubiquitination
K817 Ubiquitination
K821 Ubiquitination
K823 Ubiquitination
Y840 Phosphorylation
S855 Phosphorylation
K865 Ubiquitination
K901 Ubiquitination
K907 Ubiquitination
K918 Ubiquitination
K934 Ubiquitination
K989 Ubiquitination
K1031 Ubiquitination
S1046 Phosphorylation
S1057 Phosphorylation
K1067 Ubiquitination
S1094 Phosphorylation
S1098 Phosphorylation
K1115 Ubiquitination
S1121 Phosphorylation

研究背景

機能:

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

PTMs:

At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression.

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Golgi apparatus membrane>Multi-pass membrane protein. Cytoplasmic vesicle>COPII-coated vesicle membrane>Multi-pass membrane protein.
Note: Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed in adult and fetal tissues.

サブユニット構造:

Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Component of SCAP/SREBP complex composed of SREBF2, SCAP and RNF139; the complex hampers the interaction between SCAP and SEC24B, thereby reducing SREBF2 proteolytic processing. Interacts (via C-terminal domain) with RNF139.

タンパク質ファミリー:

Belongs to the SREBP family.

参考文献

1). NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes. LIFE SCIENCES, 2020 (PubMed: 32976883) [IF=6.1]

Application: WB    Species: rat    Sample: BRL-3A hepatocytes

Fig. 3. |NPY promotes pSREBP2 expression and accelerates the processing of pSREBP2 to mSREBP2 both in vivo and in vitro.(A) Hepatic portal vein injection of NPY (100 μg/kg) significantly increased both pSREBP2 expression and mSREBP2 maturation after 1 h in rats.
2). Digeda-4 decoction and its disassembled prescriptions improve dyslipidemia and apoptosis by regulating AMPK/SIRT1 pathway on tyloxapol-induced nonalcoholic fatty liver disease in mice. Journal of Ethnopharmacology, 2023 (PubMed: 37348794) [IF=5.4]
3). Hypermethylation of the CTRP9 promoter region promotes Hcy induced VSMC lipid deposition and foam cell formation via negatively regulating ER stress. Scientific reports, 2023 (PubMed: 37945738) [IF=4.6]
4). Sleeve Gastrectomy Suppresses Hepatic De Novo Cholesterogenesis and Improves Hepatic Cholesterol Accumulation in Obese Rats with Type 2 Diabetes Mellitus. NUTRITION, 2022 (PubMed: 34952362) [IF=4.4]
5). MiR-204-5p promotes apoptosis and inhibits migration of osteosarcoma via targeting EBF2. BIOCHIMIE, 2019 (PubMed: 30529043) [IF=3.9]

Application: WB    Species: human    Sample: MG63 cells

Figure 4.| MiR-204-5p targets EBF2 .D. WB analysis of EBF2 protein levels in MG63 cells transfected with control miRNA (miR-NC), miR-204-5p, control antagomiR (anti-miR-NC) and antagomiR-204-5p (anti-miR-204-5p).

Application: IHC    Species: human    Sample: Migrated Saos-2 and MG63 cells

Figure 5. |EBF2 inhibits apoptosis and promotes migration and invasion of osteosarcoma.E. Migrated Saos-2 and MG63 cells transfected with control siRNA (si-NC) and EBF2 siRNA (si-EBF2). Lower panels are statistics of transwell results. Data were presented as means ± SD. *=P<0.05, **=P<0.01 and ***=P<0.001
6). The Hypolipidemic Effect of Dalbergia odorifera T. C. Chen Leaf Extract on Hyperlipidemic Rats and Its Mechanism Investigation Based on Network Pharmacology. Evidence-Based Complementary and Alternative Medicine, 2021 (PubMed: 34987591)

Application: WB    Species: Rat    Sample: liver

Figure 9 Western blotting. Effect of D. odorifera leaves on the protein levels of SCAP, SREBP-2, HMGCR, and LDLR in the liver.
7). The Hypolipidemic Effect of Hawthorn Leaf Flavonoids through Modulating Lipid Metabolism and Gut Microbiota in Hyperlipidemic Rats. Evidence-based complementary and alternative medicine : eCAM, 2021 (PubMed: 36425260)

Application: WB    Species: Rat    Sample:

Figure 14 The potential drug targets for antihyperlipidemia by HLF. (a) The level of CYP7A1 in the intestinal tissue; (b) the level of HMGCR in the plasma level; (c) the expression of related targets in the liver; (d) the expression of related targets in the intestine. These data are presented as the mean ± SD (n = 3).

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