EPN2 Antibody - #AF0682
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF0682, RRID:AB_2834390.
折りたたみ／展開
EHB21; EPN 2; Epn2; EPN2_HUMAN; EPS 15 interacting protein 2; EPS-15-interacting protein 2; Eps15 binding protein; Epsin 2; Epsin-2; Epsin2; KIAA1065; OTTHUMP00000065808; OTTHUMP00000065809; OTTHUMP00000065810; OTTHUMP00000065811; OTTHUMP00000065889;
免疫原
- O95208 EPN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTSSIRRQMKNIVNNYSEAEIKVREATSNDPWGPSSSLMTEIADLTYNVVAFSEIMSMVWKRLNDHGKNWRHVYKALTLLDYLIKTGSERVAQQCRENIFAIQTLKDFQYIDRDGKDQGINVREKSKQLVALLKDEERLKAERAQALKTKERMAQVATGMGSNQITFGRGSSQPNLSTSHSEQEYGKAGGSPASYHGSPEASLCPQHRTGAPLGQSEELQPLSQRHPFLPHLGLASRPNGDWSQPCLTCDRAARATSPRVSSELEQARPQTSGEEELQLQLALAMSREVAEQEERLRRGDDLRLQMALEESRRDTVKIPKKKEHGSLPQQTTLLDLMDALPSSGPAAQKAEPWGPSASTNQTNPWGGPAAPASTSDPWPSFGTKPAASIDPWGVPTGATVQSVPKNSDPWAASQQPASSAGKRASDAWGAVSTTKPVSVSGSFELFSNLNGTIKDDFSEFDNLRTSKKTAESVTSLPSQNNGTTSPDPFESQPLTVASSKPSSARKTPESFLGPNAALVNLDSLVTRPAPPAQSLNPFLAPGAPATSAPVNPFQVNQPQPLTLNQLRGSPVLGTSTSFGPGPGVESMAVASMTSAAPQPALGATGSSLTPLGPAMMNMVGSVGIPPSAAQATGTTNPFLL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O95208 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| R170 | Methylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| S195 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T249 | Phosphorylation | Uniprot | |
| T272 | Phosphorylation | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| T316 | Phosphorylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| K423 | Acetylation | Uniprot | |
| K423 | Methylation | Uniprot | |
| K423 | Ubiquitination | Uniprot | |
| S426 | Phosphorylation | Uniprot | |
| S486 | Phosphorylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| T508 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot |
研究背景
Plays a role in the formation of clathrin-coated invaginations and endocytosis.
Ubiquitinated.
Cytoplasm. Cytoplasmic vesicle>Clathrin-coated vesicle.
Note: In punctate structures throughout the cell, associated with clathrin-coated vesicles, and particularly concentrated in the region of the Golgi complex.
Highest expression is found in brain. Detected at lower levels in lung and liver.
Binds EPS15 (By similarity). Interacts with ITSN1 (By similarity). Binds AP-2 and clathrin. Interacts with UBQLN2.
The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP-2 and clathrin binding.
Belongs to the epsin family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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