製品: ADFP Antibody
カタログ: DF7603
タンパク質の説明: Rabbit polyclonal antibody to ADFP
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Rabbit, Dog
分子量: 48 kDa; 48kD(Calculated).
ユニプロット: Q99541
RRID: AB_2841094

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(83%), Rabbit(92%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
ADFP Antibody detects endogenous levels of total ADFP.
RRID:
AB_2841094
引用形式: Affinity Biosciences Cat# DF7603, RRID:AB_2841094.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ADFP; Adipophilin; Adipose differentiation related protein; Adipose differentiation-related protein; ADRP; MGC10598; Perilipin-2; PLIN2; PLIN2_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q99541 PLIN2_HUMAN:

Milk lipid globules.

タンパク質配列:
MASVAVDPQPSVVTRVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEPQIAVANTYACKGLDRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
92
Horse
83
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99541 基板として

Site PTM Type Enzyme
A2 Acetylation
K116 Ubiquitination
K140 Ubiquitination
S148 Phosphorylation
K153 Ubiquitination
S215 Phosphorylation
Y216 Phosphorylation
Y217 Phosphorylation
S222 Phosphorylation
S224 Phosphorylation
S237 Phosphorylation
S255 Phosphorylation
K273 Ubiquitination
S291 Phosphorylation
S362 Phosphorylation
K375 Ubiquitination

研究背景

機能:

May be involved in development and maintenance of adipose tissue.

PTMs:

Acylated; primarily with C14, C16 and C18 fatty acids.

細胞の位置付け:

Membrane>Peripheral membrane protein. Lipid droplet.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Milk lipid globules.

タンパク質ファミリー:

Belongs to the perilipin family.

研究領域

· Organismal Systems > Endocrine system > PPAR signaling pathway.

参考文献

1). Preventive Effects of Ilex Cornuta Aqueous Extract on High-Fat Diet-Induced Fatty Liver of Mice. Evidence-Based Complementary and Alternative Medicine, 2022 (PubMed: 35432557)

Application: WB    Species: Mouse    Sample: liver

Figure 6 Effects of ICAE on the protein expression of PPARγ and ADRP in the liver of HFD-induced mice. The protein levels of PPARγ (a) and ADRP (b) were detected by immunoblot. Images were representative of three independent experiments, and average protein levels were quantified as ratios to β-actin, n = 5–6. ∗P < 0.05, ∗∗P < 0.01vs control; #P < 0.05, ####P < 0.0001vs. HFD group.

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