Doublecortin Antibody - #DF7608
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製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7608, RRID:AB_2841099.
折りたたみ／展開
DBCN; Dbct; DC; DCX; DCX_HUMAN; Doublecortex; Doublin; FLJ51296; Lis X; Lis-X; Lissencephalin X; Lissencephalin-X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; OTTHUMP00000023859; OTTHUMP00000023860; OTTHUMP00000216315; OTTHUMP00000216316; SCLH; XLIS;
免疫原
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
- O43602 DCX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43602 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S28 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| K50 | Acetylation | Uniprot | |
| K51 | Acetylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| Y105 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| T203 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| T222 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| S335 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| K345 | Acetylation | Uniprot | |
| K347 | Acetylation | Uniprot |
研究背景
Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).
Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.
Cytoplasm. Cell projection>Neuron projection.
Note: Localizes at neurite tips.
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
Interacts with tubulin. Interacts with USP9X.
参考文献
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