Caspase 4/11 Antibody - #DF7609

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製品: Caspase 4/11 Antibody
カタログ: DF7609
タンパク質の説明: Rabbit polyclonal antibody to Caspase 4/11
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat, Monkey
分子量: 43 kDa(Observed); 43kD(Calculated).
ユニプロット: P49662
RRID: AB_2841100

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#DF7609-BP

Caspase 4/11 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
クローナリティ:
Polyclonal
特異性:
Caspase 11 Antibody detects endogenous levels of total Caspase 11.
RRID:
AB_2841100
引用形式: Affinity Biosciences Cat# DF7609, RRID:AB_2841100.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Apoptotic cysteine protease Mih1/TX; CASP-4; CASP4; CASP4_HUMAN; Caspase 4 apoptosis related cysteine peptidase; Caspase-4 subunit 2; Caspase4; ICE(rel)-II; ICE(rel)II; ICEREL II; ICH2; Mih1/TX; Protease ICH-2; Protease TX; TX; CASP11;caspase 11;

免疫原

免疫原:

A synthesized peptide derived from human Caspase 11.

Uniprot:

P49662(CASP4_HUMAN) >>Visit HPA database.

遺伝子(ID):
CASP7(840)
発現特異性:
P49662 CASP4_HUMAN:

Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.

タンパク質配列:
MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN

研究背景

機能:

Inflammatory caspase. Essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS. Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, and IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators. Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection. In later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which ultimately results in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity. Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains. Activated by direct binding to LPS without the need of an upstream sensor. Does not directly process IL1B. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.

PTMs:

In response to activation signals, including endoplasmic reticulum stress or treatment with amyloid-beta A4 protein fragments (such as amyloid-beta protein 40), undergoes autoproteolytic cleavage.

細胞の位置付け:

Cytoplasm>Cytosol. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Mitochondrion. Inflammasome. Secreted.
Note: Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level). Not detected in brain.

タンパク質ファミリー:

The CARD domain mediates LPS recognition and homooligomerization.

Belongs to the peptidase C14A family.

研究領域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

参考文献

1). Piceatannol protects against age-related hearing loss by inhibiting cellular pyroptosis and inflammation through regulated Caspase11-GSDMD pathway. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 37100013) [IF=6.9]
2). Xuebijing injection inhibited neutrophil extracellular traps to reverse lung injury in sepsis mice via reducing Gasdermin D. Frontiers in Pharmacology, 2022 (PubMed: 36467039) [IF=5.6]

Application: WB    Species: Mice    Sample: lung tissues

"FIGURE 6 XBJ reduced the expression of caspase-11 and GSDMD in septic mice. The lung tissues were collected at 24 h after CLP. (A–C). The expressions of GSDMD and caspase-11 were determined with Western blot (A) and quantified results (B,C), respectively (n ≥ 3) (D–F). The neutrophils in lung tissues were isolated and purified at 24 h after CLP. The expressions of caspase-11 and GSDMD were detected by immunofluorescent assay as described in the methods (D). the representative images of immunofluorescent assays (E,F). The quantification of caspase-11 (E) and GSDMD (F) expressions in neutrophils isolated from lung tissues. N > 3/group. The values were indicated as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the CLP group."
3). The protective effect of isoflurane pretreatment on liver IRI by suppressing noncanonical pyroptosis of liver macrophages. International Immunopharmacology, 2021 (PubMed: 34332342) [IF=4.8]

Application: WB    Species: Mice    Sample: liver tissues

Fig. 2. Isoflurance pretreatement can attenuate liver injury by reducing noncanonical pyroptosis in liver macrophage A Caspase-11 expression in liver tissues was detected by Western biotting. B. Serum levels of IL-1β and IL-18 were measured. All data are shown as the mean ± SD n (sham) = 5 mice per group, n(IRI) = 5 mice per group *indicates statistical difference at P < 0.05.

Application: IF/ICC    Species: Mice    Sample: liver tissues

Fig. 3. Isoflurance pretreatement can attenuate liver injury by reducing noncanonical pyroptosis in liver macrophage A Representative fluorescence image of caspase-11 expression from liver sections(200x). B. Serum levels of IL-1β and IL-18 were measured. All data are shown as the mean ± SD n (sham) = 5 mice per group, n(IRI) = 5 mice per group *indicates statistical difference at P < 0.05.
4). Proteomic analysis reveals that ACSL4 activation during reflux esophagitis contributes to ferroptosis-mediated esophageal mucosal damage. European Journal of Pharmacology, 2022 (PubMed: 35921957) [IF=4.2]
5). NLRP3/Caspase-1-Mediated Pyroptosis of Astrocytes Induced by Antipsychotics Is Inhibited by a Histamine H1 Receptor-Selective Agonist. Frontiers in Aging Neuroscience, 2022 (PubMed: 35615587) [IF=4.1]

Application: WB    Species: Human    Sample:

FIGURE 2 Olanzapine treatment activated NLRP3/caspase-1 signaling, and increased NLRC4, NLRP6, and IL-1β protein expression in cultured astrocytes. Cultured cells were treated with 0.1, 1, or 10 μM olanzapine for 24 h. (A–E) Western blot was used to evaluate the expression of NLRP3, caspase-1 (20 kDa), GSDMD (53 and 35 kDa), NLRC4, NLRP6, and IL-1β. The data are presented as the mean ± standard error of the mean. *p < 0.05, **p < 0.01, ***p < 0.001 vs. control (one-way analysis of variance and post hoc Dunnett’s multiple comparison test; n = 3–4 cultures/western blot group). Con, control; OLZ, olanzapine.
6). Esketamine mitigates endotoxin-induced acute lung injury by suppressing caspase-11-driven pyroptosis. BMC anesthesiology, 2025 (PubMed: 40691531) [IF=2.3]

Application: WB    Species: Rat    Sample: lung tissue

Fig. 4 A: The expression of caspase-11 and GSDMD-N protein in each group was detected by WB, caspase-11: CLP vs. Sham (P 

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