BIRC3 Antibody - #DF7625
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7625, RRID:AB_2841111.
折りたたみ/展開
AIP 1; AIP1; API 2; API2; Apoptosis inhibitor 2; Baculoviral IAP repeat containing 3; Baculoviral IAP repeat containing protein 3; Baculoviral IAP repeat-containing protein 3; BIRC 3; BIRC3; BIRC3_HUMAN; C IAP2; C-IAP2; CIAP 2; CIAP2; HAIP 1; HAIP1; HIAP 1; HIAP-1; HIAP1; IAP homolog C; IAP-1; Inhibitor of apoptosis protein 1; MALT 2; MALT2; Mammalian IAP homolog C; MIHC; RING finger protein 49; RNF49; TNFR2 TRAF signaling complex protein 1; TNFR2 TRAF signalling complex protein; TNFR2-TRAF-signaling complex protein 1;
免疫原
Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
- Q13489 BIRC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13489 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S7 | Phosphorylation | Uniprot | |
S11 | Phosphorylation | Uniprot | |
K63 | Ubiquitination | Uniprot | |
S141 | Phosphorylation | Uniprot | |
K211 | Ubiquitination | Uniprot | |
K218 | Ubiquitination | Uniprot | |
K231 | Ubiquitination | Uniprot | |
Y245 | Phosphorylation | Uniprot | |
S248 | Phosphorylation | Uniprot | |
T254 | Phosphorylation | Uniprot | |
K260 | Ubiquitination | Uniprot | |
S278 | Phosphorylation | Uniprot | |
K396 | Ubiquitination | Uniprot | |
T398 | Phosphorylation | Uniprot | |
K402 | Ubiquitination | Uniprot | |
K439 | Ubiquitination | Uniprot | |
K485 | Ubiquitination | Uniprot | |
S540 | Phosphorylation | Uniprot | |
K587 | Ubiquitination | Uniprot | |
K596 | Ubiquitination | Uniprot |
研究背景
Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, IKBKE, TRAF1, and BCL10. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8.
Auto-ubiquitinated and degraded by the proteasome in apoptotic cells.
Cytoplasm. Nucleus.
Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.
Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. The BIR motifs region interacts with TNF receptor associated factors 1 and 2 (TRAF1 and TRAF2) to form a heteromeric complex, which is then recruited to the tumor necrosis factor receptor 2 (TNFR2). Interaction with TRAF2 is required for ubiquitination of IKBKE, degradation of NFKBIA and activation of NF-kappa-B. Interacts with RIP1, RIP2, RIP3, RIP4 and USP19.
Belongs to the IAP family.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
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