PLEC Antibody - #DF7631

(1)  
製品: PLEC Antibody
カタログ: DF7631
タンパク質の説明: Rabbit polyclonal antibody to PLEC
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse
分子量: 531 KD; 532kD(Calculated).
ユニプロット: Q15149
RRID: AB_2841116

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%)
クローナリティ:
Polyclonal
特異性:
PLEC Antibody detects endogenous levels of total PLEC.
RRID:
AB_2841116
引用形式: Affinity Biosciences Cat# DF7631, RRID:AB_2841116.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

EBS1; EBSO; HD1; Hemidesmosomal protein 1; PCN; pleC; PLEC_HUMAN; PLEC1; PLEC1b; Plectin 1; Plectin 1 intermediate filament binding protein 500kDa; Plectin 6; Plectin; Plectin-1; PLTN;

免疫原

免疫原:
Uniprot:

Q15149(PLEC_HUMAN) >>Visit HPA database.

遺伝子(ID):
PLEC(5339)
発現特異性:
Q15149 PLEC_HUMAN:

Widely expressed with highest levels in muscle, heart, placenta and spinal cord.

タンパク質配列:
MVAGMLMPRDQLRAIYEVLFREGVMVAKKDRRPRSLHPHVPGVTNLQVMRAMASLRARGLVRETFAWCHFYWYLTNEGIAHLRQYLHLPPEIVPASLQRVRRPVAMVMPARRTPHVQAVQGPLGSPPKRGPLPTEEQRVYRRKELEEVSPETPVVPATTQRTLARPGPEPAPATDERDRVQKKTFTKWVNKHLIKAQRHISDLYEDLRDGHNLISLLEVLSGDSLPREKGRMRFHKLQNVQIALDYLRHRQVKLVNIRNDDIADGNPKLTLGLIWTIILHFQISDIQVSGQSEDMTAKEKLLLWSQRMVEGYQGLRCDNFTSSWRDGRLFNAIIHRHKPLLIDMNKVYRQTNLENLDQAFSVAERDLGVTRLLDPEDVDVPQPDEKSIITYVSSLYDAMPRVPDVQDGVRANELQLRWQEYRELVLLLLQWMRHHTAAFEERRFPSSFEEIEILWSQFLKFKEMELPAKEADKNRSKGIYQSLEGAVQAGQLKVPPGYHPLDVEKEWGKLHVAILEREKQLRSEFERLECLQRIVTKLQMEAGLCEEQLNQADALLQSDVRLLAAGKVPQRAGEVERDLDKADSMIRLLFNDVQTLKDGRHPQGEQMYRRVYRLHERLVAIRTEYNLRLKAGVAAPATQVAQVTLQSVQRRPELEDSTLRYLQDLLAWVEENQHRVDGAEWGVDLPSVEAQLGSHRGLHQSIEEFRAKIERARSDEGQLSPATRGAYRDCLGRLDLQYAKLLNSSKARLRSLESLHSFVAAATKELMWLNEKEEEEVGFDWSDRNTNMTAKKESYSALMRELELKEKKIKELQNAGDRLLREDHPARPTVESFQAALQTQWSWMLQLCCCIEAHLKENAAYFQFFSDVREAEGQLQKLQEALRRKYSCDRSATVTRLEDLLQDAQDEKEQLNEYKGHLSGLAKRAKAVVQLKPRHPAHPMRGRLPLLAVCDYKQVEVTVHKGDECQLVGPAQPSHWKVLSSSGSEAAVPSVCFLVPPPNQEAQEAVTRLEAQHQALVTLWHQLHVDMKSLLAWQSLRRDVQLIRSWSLATFRTLKPEEQRQALHSLELHYQAFLRDSQDAGGFGPEDRLMAEREYGSCSHHYQQLLQSLEQGAQEESRCQRCISELKDIRLQLEACETRTVHRLRLPLDKEPARECAQRIAEQQKAQAEVEGLGKGVARLSAEAEKVLALPEPSPAAPTLRSELELTLGKLEQVRSLSAIYLEKLKTISLVIRGTQGAEEVLRAHEEQLKEAQAVPATLPELEATKASLKKLRAQAEAQQPTFDALRDELRGAQEVGERLQQRHGERDVEVERWRERVAQLLERWQAVLAQTDVRQRELEQLGRQLRYYRESADPLGAWLQDARRRQEQIQAMPLADSQAVREQLRQEQALLEEIERHGEKVEECQRFAKQYINAIKDYELQLVTYKAQLEPVASPAKKPKVQSGSESVIQEYVDLRTHYSELTTLTSQYIKFISETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKELQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRIEEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVALETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAREEAERELERWQLKANEALRLRLQAEEVAQQKSLAQAEAEKQKEEAEREARRRGKAEEQAVRQRELAEQELEKQRQLAEGTAQQRLAAEQELIRLRAETEQGEQQRQLLEEELARLQREAAAATQKRQELEAELAKVRAEMEVLLASKARAEEESRSTSEKSKQRLEAEAGRFRELAEEAARLRALAEEAKRQRQLAEEDAARQRAEAERVLAEKLAAIGEATRLKTEAEIALKEKEAENERLRRLAEDEAFQRRRLEEQAAQHKADIEERLAQLRKASDSELERQKGLVEDTLRQRRQVEEEILALKASFEKAAAGKAELELELGRIRSNAEDTLRSKEQAELEAARQRQLAAEEERRRREAEERVQKSLAAEEEAARQRKAALEEVERLKAKVEEARRLRERAEQESARQLQLAQEAAQKRLQAEEKAHAFAVQQKEQELQQTLQQEQSVLDQLRGEAEAARRAAEEAEEARVQAEREAAQSRRQVEEAERLKQSAEEQAQARAQAQAAAEKLRKEAEQEAARRAQAEQAALRQKQAADAEMEKHKKFAEQTLRQKAQVEQELTTLRLQLEETDHQKNLLDEELQRLKAEATEAARQRSQVEEELFSVRVQMEELSKLKARIEAENRALILRDKDNTQRFLQEEAEKMKQVAEEAARLSVAAQEAARLRQLAEEDLAQQRALAEKMLKEKMQAVQEATRLKAEAELLQQQKELAQEQARRLQEDKEQMAQQLAEETQGFQRTLEAERQRQLEMSAEAERLKLRVAEMSRAQARAEEDAQRFRKQAEEIGEKLHRTELATQEKVTLVQTLEIQRQQSDHDAERLREAIAELEREKEKLQQEAKLLQLKSEEMQTVQQEQLLQETQALQQSFLSEKDSLLQRERFIEQEKAKLEQLFQDEVAKAQQLREEQQRQQQQMEQERQRLVASMEEARRRQHEAEEGVRRKQEELQQLEQQRRQQEELLAEENQRLREQLQLLEEQHRAALAHSEEVTASQVAATKTLPNGRDALDGPAAEAEPEHSFDGLRRKVSAQRLQEAGILSAEELQRLAQGHTTVDELARREDVRHYLQGRSSIAGLLLKATNEKLSVYAALQRQLLSPGTALILLEAQAASGFLLDPVRNRRLTVNEAVKEGVVGPELHHKLLSAERAVTGYKDPYTGQQISLFQAMQKGLIVREHGIRLLEAQIATGGVIDPVHSHRVPVDVAYRRGYFDEEMNRVLADPSDDTKGFFDPNTHENLTYLQLLERCVEDPETGLCLLPLTDKAAKGGELVYTDSEARDVFEKATVSAPFGKFQGKTVTIWEIINSEYFTAEQRRDLLRQFRTGRITVEKIIKIIITVVEEQEQKGRLCFEGLRSLVPAAELLESRVIDRELYQQLQRGERSVRDVAEVDTVRRALRGANVIAGVWLEEAGQKLSIYNALKKDLLPSDMAVALLEAQAGTGHIIDPATSARLTVDEAVRAGLVGPEFHEKLLSAEKAVTGYRDPYTGQSVSLFQALKKGLIPREQGLRLLDAQLSTGGIVDPSKSHRVPLDVACARGCLDEETSRALSAPRADAKAYSDPSTGEPATYGELQQRCRPDQLTGLSLLPLSEKAARARQEELYSELQARETFEKTPVEVPVGGFKGRTVTVWELISSEYFTAEQRQELLRQFRTGKVTVEKVIKILITIVEEVETLRQERLSFSGLRAPVPASELLASGVLSRAQFEQLKDGKTTVKDLSELGSVRTLLQGSGCLAGIYLEDTKEKVSIYEAMRRGLLRATTAALLLEAQAATGFLVDPVRNQRLYVHEAVKAGVVGPELHEQLLSAEKAVTGYRDPYSGSTISLFQAMQKGLVLRQHGIRLLEAQIATGGIIDPVHSHRVPVDVAYQRGYFSEEMNRVLADPSDDTKGFFDPNTHENLTYRQLLERCVEDPETGLRLLPLKGAEKAEVVETTQVYTEEETRRAFEETQIDIPGGGSHGGSTMSLWEVMQSDLIPEEQRAQLMADFQAGRVTKERMIIIIIEIIEKTEIIRQQGLASYDYVRRRLTAEDLFEARIISLETYNLLREGTRSLREALEAESAWCYLYGTGSVAGVYLPGSRQTLSIYQALKKGLLSAEVARLLLEAQAATGFLLDPVKGERLTVDEAVRKGLVGPELHDRLLSAERAVTGYRDPYTEQTISLFQAMKKELIPTEEALRLLDAQLATGGIVDPRLGFHLPLEVAYQRGYLNKDTHDQLSEPSEVRSYVDPSTDERLSYTQLLRRCRRDDGTGQLLLPLSDARKLTFRGLRKQITMEELVRSQVMDEATALQLREGLTSIEEVTKNLQKFLEGTSCIAGVFVDATKERLSVYQAMKKGIIRPGTAFELLEAQAATGYVIDPIKGLKLTVEEAVRMGIVGPEFKDKLLSAERAVTGYKDPYSGKLISLFQAMKKGLILKDHGIRLLEAQIATGGIIDPEESHRLPVEVAYKRGLFDEEMNEILTDPSDDTKGFFDPNTEENLTYLQLMERCITDPQTGLCLLPLKEKKRERKTSSKSSVRKRRVVIVDPETGKEMSVYEAYRKGLIDHQTYLELSEQECEWEEITISSSDGVVKSMIIDRRSGRQYDIDDAIAKNLIDRSALDQYRAGTLSITEFADMLSGNAGGFRSRSSSVGSSSSYPISPAVSRTQLASWSDPTEETGPVAGILDTETLEKVSITEAMHRNLVDNITGQRLLEAQACTGGIIDPSTGERFPVTDAVNKGLVDKIMVDRINLAQKAFCGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPLDEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEAAAQSTKGYYSPYSVSGSGSTAGSRTGSRTGSRAGSRRGSFDATGSGFSMTFSSSSYSSSGYGRRYASGSSASLGGPESAVA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15149 基板として

Site PTM Type Enzyme
R9 Methylation
Y16 Phosphorylation
K28 Ubiquitination
T113 Phosphorylation
S125 Phosphorylation
K128 Ubiquitination
K143 Ubiquitination
S149 Phosphorylation
T152 Phosphorylation
T159 Phosphorylation
K187 Acetylation
K187 Ubiquitination
K191 Acetylation
K191 Ubiquitination
K195 Ubiquitination
S201 Phosphorylation
Y204 Phosphorylation
S224 Phosphorylation
K236 Acetylation
K236 Ubiquitination
Y246 Phosphorylation
K253 Ubiquitination
S284 Phosphorylation
K300 Ubiquitination
S305 Phosphorylation
Y312 Phosphorylation
K338 Ubiquitination
K346 Acetylation
T351 Phosphorylation
S361 Phosphorylation
T370 Phosphorylation
K386 Ubiquitination
Y396 Phosphorylation
K462 Acetylation
K469 Ubiquitination
K477 Ubiquitination
Y480 Phosphorylation
S482 Phosphorylation
K493 Ubiquitination
K505 Acetylation
K567 Ubiquitination
K581 Ubiquitination
S584 Phosphorylation
K597 Ubiquitination
Y608 Phosphorylation
K630 Ubiquitination
T638 Phosphorylation
S647 Phosphorylation
R650 Methylation
R651 Methylation
S687 Phosphorylation
S701 Phosphorylation
S714 Phosphorylation
S720 Phosphorylation
K740 Acetylation
K740 Ubiquitination
K746 Ubiquitination
S757 Phosphorylation
S782 Phosphorylation
K792 Ubiquitination
S794 Phosphorylation
Y795 Phosphorylation
S796 Phosphorylation
K805 Ubiquitination
Y861 Phosphorylation
K877 Acetylation
K877 Ubiquitination
S887 Phosphorylation
S891 Phosphorylation
T893 Phosphorylation
K908 Acetylation
K908 Ubiquitination
Y914 Phosphorylation
K915 Methylation
K915 Ubiquitination
S919 Phosphorylation
K923 Ubiquitination
K926 Methylation
K932 Ubiquitination
C950 S-Nitrosylation
K953 Acetylation
K977 Methylation
S1029 Phosphorylation
S1047 Phosphorylation
T1050 Phosphorylation
Y1070 Phosphorylation
S1077 Phosphorylation
R1088 Methylation
C1098 S-Nitrosylation
S1124 Phosphorylation
C1136 S-Nitrosylation
K1150 Ubiquitination
K1165 Ubiquitination
K1175 Ubiquitination
K1186 Acetylation
K1186 Ubiquitination
S1194 Phosphorylation
S1202 Phosphorylation
K1210 Ubiquitination
S1218 Phosphorylation
Y1221 Phosphorylation
K1224 Acetylation
K1224 Ubiquitination
T1227 Phosphorylation
K1250 Ubiquitination
K1266 Acetylation
K1266 Ubiquitination
R1291 Methylation
S1352 Phosphorylation
K1410 Acetylation
K1410 Ubiquitination
Y1412 Phosphorylation
K1417 Ubiquitination
Y1419 Phosphorylation
Y1426 Phosphorylation
K1427 Ubiquitination
S1435 Phosphorylation
K1438 Ubiquitination
S1444 Phosphorylation
S1446 Phosphorylation
Y1453 Phosphorylation
S1475 Phosphorylation
T1477 Phosphorylation
K1508 Ubiquitination
K1552 Ubiquitination
S1554 Phosphorylation
S1565 Phosphorylation
K1573 Ubiquitination
R1605 Methylation
R1627 Methylation
R1637 Methylation
S1644 Phosphorylation
S1717 Phosphorylation
K1718 Ubiquitination
S1721 Phosphorylation
K1725 Acetylation
K1725 Ubiquitination
T1726 Phosphorylation
S1732 Phosphorylation
K1774 Acetylation
K1792 Ubiquitination
R1812 Methylation
K1896 Acetylation
S1907 Phosphorylation
K1908 Acetylation
R1942 Methylation
K1951 Acetylation
K1975 Acetylation
T1983 Phosphorylation
K2025 Ubiquitination
S2039 Phosphorylation
K2047 Ubiquitination
T2053 Phosphorylation
K2073 Acetylation
K2078 Ubiquitination
K2142 Ubiquitination
K2182 Acetylation
K2182 Ubiquitination
K2189 Acetylation
K2198 Ubiquitination
K2255 Acetylation
K2274 Acetylation
K2274 Ubiquitination
K2339 Ubiquitination
K2350 Ubiquitination
T2354 Phosphorylation
S2361 Phosphorylation
S2369 Phosphorylation
S2378 Phosphorylation
K2379 Acetylation
K2379 Ubiquitination
K2409 Ubiquitination
K2447 Ubiquitination
K2473 Ubiquitination
S2516 Phosphorylation
K2553 Acetylation
S2578 Phosphorylation
K2604 Ubiquitination
K2609 Ubiquitination
T2615 Phosphorylation
S2631 Phosphorylation
K2650 Ubiquitination
K2652 Ubiquitination
K2663 Acetylation
K2663 Ubiquitination
S2688 Phosphorylation
K2706 Ubiquitination
S2749 Phosphorylation
S2755 Phosphorylation
K2761 Ubiquitination
S2782 Phosphorylation
S2791 Phosphorylation
S2802 Phosphorylation
T2814 Phosphorylation
T2815 Phosphorylation
S2834 Phosphorylation
K2841 Acetylation
K2841 Ubiquitination
T2886 Phosphorylation Q96GD4 (AURKB)
K2915 Ubiquitination
K2931 Ubiquitination
T2949 Phosphorylation
Y2967 Phosphorylation
Y2971 Phosphorylation
S2984 Phosphorylation
K2988 Ubiquitination
T3000 Phosphorylation
Y3001 Phosphorylation
K3024 Ubiquitination
K3027 Ubiquitination
Y3033 Phosphorylation
T3034 Phosphorylation
S3036 Phosphorylation
K3044 Acetylation
K3044 Ubiquitination
K3053 Acetylation
K3053 Ubiquitination
T3084 Phosphorylation
K3091 Acetylation
S3116 Phosphorylation
S3126 Phosphorylation
Y3134 Phosphorylation
S3143 Phosphorylation
T3152 Phosphorylation
Y3178 Phosphorylation
K3183 Ubiquitination
S3188 Phosphorylation
S3210 Phosphorylation
K3231 Acetylation
K3231 Ubiquitination
K3237 Ubiquitination
S3252 Phosphorylation
S3276 Phosphorylation
T3277 Phosphorylation
S3284 Phosphorylation
K3285 Ubiquitination
C3295 S-Nitrosylation
C3299 S-Nitrosylation
S3309 Phosphorylation
K3316 Ubiquitination
T3328 Phosphorylation
Y3329 Phosphorylation
C3336 S-Nitrosylation
K3352 Ubiquitination
Y3362 Phosphorylation
S3363 Phosphorylation
K3373 Ubiquitination
T3374 Phosphorylation
K3384 Ubiquitination

研究背景

機能:

Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.

PTMs:

Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Cell junction>Hemidesmosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed with highest levels in muscle, heart, placenta and spinal cord.

サブユニット構造:

Homodimer or homotetramer. Interacts (via actin-binding domain) with SYNE3. Interacts (via calponin-homology (CH) 1 domain) with VIM (via rod region) (By similarity). Interacts (via N-terminus) with DST isoform 2 (via N-terminus). Interacts with FER. Interacts with TOR1A. Interacts with ANK3. Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Interacts with COL17A1.

タンパク質ファミリー:

The N-terminus interacts with actin, the C-terminus with vimentin, desmin, GFAP, cytokeratins, lamin B; whereas both the N- and the C-terminus can bind integrin beta-4.

Belongs to the plakin or cytolinker family.

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