CCNA2 Antibody - #DF7639
| 製品: | CCNA2 Antibody |
| カタログ: | DF7639 |
| タンパク質の説明: | Rabbit polyclonal antibody to CCNA2 |
| アプリケーション: | WB |
| 反応性: | Human |
| 分子量: | 60 kDa; 49kD(Calculated). |
| ユニプロット: | P20248 |
| RRID: | AB_2841120 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7639, RRID:AB_2841120.
折りたたみ／展開
CCN1; CCNA; Ccna2; CCNA2_HUMAN; Cyclin A2; Cyclin-A; Cyclin-A2;
免疫原
A synthesized peptide derived from human CCNA2, corresponding to a region within C-terminal amino acids.
- P20248 CCNA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLGNSAPGPATREAGSALLALQQTALQEDQENINPEKAAPVQQPRTRAALAVLKSGNPRGLAQQQRPKTRRVAPLKDLPVNDEHVTVPPWKANSKQPAFTIHVDEAEKEAQKKPAESQKIEREDALAFNSAISLPGPRKPLVPLDYPMDGSFESPHTMDMSIILEDEKPVSVNEVPDYHEDIHTYLREMEVKCKPKVGYMKKQPDITNSMRAILVDWLVEVGEEYKLQNETLHLAVNYIDRFLSSMSVLRGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL
PTMs - P20248 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K54 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K68 | Acetylation | Uniprot | |
| K76 | Acetylation | Uniprot | |
| K95 | Acetylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K112 | Acetylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K192 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | Uniprot | |
| Y225 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T380 | Phosphorylation | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K417 | Ubiquitination | Uniprot |
研究背景
Cyclin which controls both the G1/S and the G2/M transition phases of the cell cycle. Functions through the formation of specific serine/threonine protein kinase holoenzyme complexes with the cyclin-dependent protein kinases CDK1 or CDK2. The cyclin subunit confers the substrate specificity of these complexes and differentially interacts with and activates CDK1 and CDK2 throughout the cell cycle.
Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
Nucleus. Cytoplasm.
Note: Exclusively nuclear during interphase (PubMed:1312467). Detected in the nucleus and the cytoplasm at prophase (PubMed:1312467). Cytoplasmic when associated with SCAPER (PubMed:17698606).
Interacts with the CDK1 and CDK2 protein kinases to form serine/threonine kinase holoenzyme complexes. Interacts with CDK1 (hyperphosphorylated form in G1 and underphosphorylated forms in S and G2). Interacts with CDK2; the interaction increases from G1 to G2. Interacts (associated with CDK2 but not with CDK1) with SCAPER; regulates the activity of CCNA2/CDK2 by transiently maintaining CCNA2 in the cytoplasm. Forms a ternary complex with CDK2 and CDKN1B; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements. Interacts with INCA1.
(Microbial infection) Interacts with human cytomegalovirus protein UL32.
Belongs to the cyclin family. Cyclin AB subfamily.
研究領域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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