Caspase 5 Antibody - #DF7664

(1)   (3)  
製品: Caspase 5 Antibody
カタログ: DF7664
タンパク質の説明: Rabbit polyclonal antibody to Caspase 5
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 47 kDa; 50kD(Calculated).
ユニプロット: P51878
RRID: AB_2841138

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Caspase 5 Antibody detects endogenous levels of total Caspase 5.
RRID:
AB_2841138
引用形式: Affinity Biosciences Cat# DF7664, RRID:AB_2841138.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Apoptosis related cysteine protease; CASP-5; CASP5; CASP5_HUMAN; Caspase-5 subunit p10; ICE(rel)-III; ICERELIII; ICH 3; ICH 3 protease; Protease ICH-3; Protease TY; TY; TY protease;

免疫原

免疫原:
Uniprot:

P51878(CASP5_HUMAN) >>Visit HPA database.

遺伝子(ID):
CASP5(838)
発現特異性:
P51878 CASP5_HUMAN:

Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

タンパク質配列:
MAEDSGKKKRRKNFEAMFKGILQSGLDNFVINHMLKNNVAGQTSIQTLVPNTDQKSTSVKKDNHKKKTVKMLEYLGKDVLHGVFNYLAKHDVLTLKEEEKKKYYDTKIEDKALILVDSLRKNRVAHQMFTQTLLNMDQKITSVKPLLQIEAGPPESAESTNILKLCPREEFLRLCKKNHDEIYPIKKREDRRRLALIICNTKFDHLPARNGAHYDIVGMKRLLQGLGYTVVDEKNLTARDMESVLRAFAARPEHKSSDSTFLVLMSHGILEGICGTAHKKKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGEKHGELWVRDSPASLALISSQSSENLEADSVCKIHEEKDFIAFCSSTPHNVSWRDRTRGSIFITELITCFQKYSCCCHLMEIFRKVQKSFEVPQAKAQMPTIERATLTRDFYLFPGN

PTMs - P51878 基板として

Site PTM Type Enzyme
S44 Phosphorylation
Y74 Phosphorylation
Y103 Phosphorylation
Y104 Phosphorylation
Y228 Phosphorylation
T229 Phosphorylation
T237 Phosphorylation
K282 Ubiquitination

研究背景

機能:

Mediator of programmed cell death (apoptosis). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.

PTMs:

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

組織特異性:

Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle.

サブユニット構造:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits. Interacts with MEFV.Interacts with NOD2. Interacts with SERPINB1; this interaction regulates CASP5 activity.

タンパク質ファミリー:

Belongs to the peptidase C14A family.

研究領域

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

参考文献

1). RBM15-mediated N6-methyladenosine modification affects COVID-19 severity by regulating the expression of multitarget genes. Cell death & disease, 2021 (PubMed: 34301919) [IF=9.0]

Application: WB    Species: Human    Sample:

Fig. 2 Aberrant methylation triggered by SARS-CoV-2 regulates multiple targets related to immune response and cell death pathways. Heatmap of genes (P 1.5) related to the cell death (A) and immune response (B). Box plot (C) depicting the expression levels of CASP1, CASP5, DDX3X, TRIB1, IL17RB, and TLSP in patients with COVID-19 (20 severe and 19 mild patients) as compared with those in healthy controls (n = 20). And scatter plots revealed m6A enrichment of CASP1, CASP5, DDX3X, TRIB1, IL17RB, and TSLP in patients. D Total m6A levels of COVID-19 patients and healthy controls (n = 3). E The protein levels of candidate genes were detected by western blot in COVID-19 patients and healthy controls (HCs). F CASP1, CASP5, DDX3X, TRIB1, IL17RB, and TLSP expression levels in HuT 78 cells co-cultured with activated THP-1 cells were measured after being treated with S proteins separately for 0, 6, 12, and 24 h. HuT 78 cells were co-cultured with (Ctrl) or without (spike) spike proteins (50 ng/ml), and cell death was detected by apoptosis assay (G), quantitative analysis of positive signals was showed in (H), and the protein levels of cleaved–CASP3 were detected using western blot (I). CCK-8 assay (J) was applied to evaluate proliferation abilities of HuT 78 cells with spike stimulated or without spike. Data were shown as means ± SD (n = 3, *P 

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.