MEK5 Antibody - #DF7667
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| 製品: | MEK5 Antibody |
| カタログ: | DF7667 |
| タンパク質の説明: | Rabbit polyclonal antibody to MEK5 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 50kDa; 50kD(Calculated). |
| ユニプロット: | Q13163 |
| RRID: | AB_2841140 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7667, RRID:AB_2841140.
折りたたみ／展開
Dual specificity mitogen activated protein kinase kinase 5; Dual specificity mitogen-activated protein kinase kinase 5; EC 2.7.12.2; HsT17454; MAP kinase kinase 5; MAP kinase kinase MEK5b; MAP2K5; MAPK/ERK kinase 5; MAPKK 5; MAPKK5; MEK 5; mitogen-activated protein kinase kinase 5; MKK5; MP2K5_HUMAN; PRKMK5; Protein kinase, mitogen-activated, kinase 5; SAPKK5; SKK5;
免疫原
- Q13163 MP2K5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLWLALGPFPAMENQVLVIRIKIPNSGAVDWTVHSGPQLLFRDVLDVIGQVLPEATTTAFEYEDEDGDRITVRSDEEMKAMLSYYYSTVMEQQVNGQLIEPLQIFPRACKPPGERNIHGLKVNTRAGPSQHSSPAVSDSLPSNSLKKSSAELKKILANGQMNEQDIRYRDTLGHGNGGTVYKAYHVPSGKILAVKVILLDITLELQKQIMSELEILYKCDSSYIIGFYGAFFVENRISICTEFMDGGSLDVYRKMPEHVLGRIAVAVVKGLTYLWSLKILHRDVKPSNMLVNTRGQVKLCDFGVSTQLVNSIAKTYVGTNAYMAPERISGEQYGIHSDVWSLGISFMELALGRFPYPQIQKNQGSLMPLQLLQCIVDEDSPVLPVGEFSEPFVHFITQCMRKQPKERPAPEELMGHPFIVQFNDGNAAVVSMWVCRALEERRSQQGPP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13163 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T71 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| R125 | Methylation | Uniprot | |
| S129 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S133 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| S142 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| K146 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S149 | Phosphorylation | Q13164 (MAPK7) | Uniprot |
| K154 | Ubiquitination | Uniprot | |
| K298 | Ubiquitination | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| T315 | Phosphorylation | Uniprot |
研究背景
Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appears to play a critical role in protecting cells from stress-induced apoptosis, neuronal survival and cardiac development and angiogenesis.
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
Expressed in many adult tissues. Abundant in heart and skeletal muscle.
Interacts with PARD6A, MAP3K3 and MAPK7. Forms a complex with SQSTM1 and PRKCZ or PRKCI (By similarity). Interacts with Yersinia yopJ.
Binds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the PB1 domain. This domain also mediates interactions with SQSTM1 and PARD6A (By similarity).
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
参考文献
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