NEFM Antibody - #DF7673
| 製品: | NEFM Antibody |
| カタログ: | DF7673 |
| タンパク質の説明: | Rabbit polyclonal antibody to NEFM |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 150 KD; 102kD(Calculated). |
| ユニプロット: | P07197 |
| RRID: | AB_2841145 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7673, RRID:AB_2841145.
折りたたみ／展開
150kDa medium; NEF3; NEFM; Neurofilament 3; NF160; NFM;
免疫原
- P07197 NFM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSMLAPRLAYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVHYLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEEDIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNHEEEVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLTEAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQDTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSITGPLYTHRPPITISSKIQKPKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEELAVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEEDEGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEELVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEKKEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGEGGSEEEGSDKGAKGSRKEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPADEKKGGDKSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKVEKVTSHAIVKEVTQSD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P07197 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R21 | Methylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| R26 | Methylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| R42 | Methylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| R54 | Methylation | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| R111 | Methylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| R382 | Methylation | Uniprot | |
| Y384 | Phosphorylation | Uniprot | |
| Y401 | Phosphorylation | Uniprot | |
| K445 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| S467 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S545 | Phosphorylation | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| S615 | Phosphorylation | Uniprot | |
| S620 | Phosphorylation | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S641 | Phosphorylation | Uniprot | |
| S646 | Phosphorylation | Uniprot | |
| K653 | Acetylation | Uniprot | |
| S654 | Phosphorylation | Uniprot | |
| S659 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| S680 | Phosphorylation | Uniprot | |
| S685 | Phosphorylation | Uniprot | |
| K693 | Acetylation | Uniprot | |
| K698 | Acetylation | Uniprot | |
| S736 | Phosphorylation | Uniprot | |
| T748 | Phosphorylation | Uniprot | |
| T750 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| S788 | Phosphorylation | Uniprot | |
| S821 | Phosphorylation | Uniprot | |
| S837 | Phosphorylation | Uniprot | |
| K871 | Ubiquitination | Uniprot | |
| Y872 | Phosphorylation | Uniprot | |
| K875 | Ubiquitination | Uniprot |
研究背景
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.
Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Belongs to the intermediate filament family.
研究領域
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
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