製品: DNAJC3 Antibody
カタログ: DF7685
タンパク質の説明: Rabbit polyclonal antibody to DNAJC3
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 54 kDa; 58kD(Calculated).
ユニプロット: Q13217
RRID: AB_2841156

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(92%), Horse(100%), Sheep(100%), Dog(100%), Chicken(91%), Xenopus(91%)
クローナリティ:
Polyclonal
特異性:
DNAJC3 Antibody detects endogenous levels of total DNAJC3.
RRID:
AB_2841156
引用形式: Affinity Biosciences Cat# DF7685, RRID:AB_2841156.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

DnaJ (Hsp40) homolog subfamily C member 3; DnaJ homolog subfamily C member 3; DNAJC3; DNJC3_HUMAN; double-stranded RNA-activated protein kinase inhibitor; Endoplasmic reticulum DnaJ protein 6; ERdj6; HP58; Interferon induced, double stranded RNA activated protein kinase inhibitor; Interferon-induced; P58; p58 ipk; P58IPK; PRKRI; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58; Protein kinase interferon inducible double stranded RNA dependent inhibitor;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q13217 DNJC3_HUMAN:

Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.

タンパク質配列:
MVAPGSVTSRLGSVFPFLLVLVDLQYEGAECGVNADVEKHLELGKKLLAAGQLADALSQFHAAVDGDPDNYIAYYRRATVFLAMGKSKAALPDLTKVIQLKMDFTAARLQRGHLLLKQGKLDEAEDDFKKVLKSNPSENEEKEAQSQLIKSDEMQRLRSQALNAFGSGDYTAAIAFLDKILEVCVWDAELRELRAECFIKEGEPRKAISDLKAASKLKNDNTEAFYKISTLYYQLGDHELSLSEVRECLKLDQDHKRCFAHYKQVKKLNKLIESAEELIRDGRYTDATSKYESVMKTEPSIAEYTVRSKERICHCFSKDEKPVEAIRVCSEVLQMEPDNVNALKDRAEAYLIEEMYDEAIQDYETAQEHNENDQQIREGLEKAQRLLKQSQKRDYYKILGVKRNAKKQEIIKAYRKLALQWHPDNFQNEEEKKKAEKKFIDIAAAKEVLSDPEMRKKFDDGEDPLDAESQQGGGGNPFHRSWNSWQGFNPFSSGGPFRFKFHFN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Bovine
92
Xenopus
91
Chicken
91
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13217 基板として

Site PTM Type Enzyme
S6 Phosphorylation
T8 Phosphorylation
S9 Phosphorylation
K46 Methylation
K88 Ubiquitination
T95 Phosphorylation
K96 Ubiquitination
K117 Ubiquitination
K133 Ubiquitination
K142 Ubiquitination
K150 Ubiquitination
S159 Phosphorylation
Y170 Phosphorylation
K200 Ubiquitination
K212 Ubiquitination
K218 Ubiquitination
Y232 Phosphorylation
K270 Ubiquitination
S274 Phosphorylation
K290 Ubiquitination
K296 Ubiquitination
Y304 Phosphorylation
T305 Phosphorylation
K318 Ubiquitination
K344 Ubiquitination
K382 Ubiquitination
K388 Ubiquitination
K407 Methylation
K412 Ubiquitination
K416 Ubiquitination
K446 Ubiquitination
S450 Phosphorylation
K457 Ubiquitination

研究背景

機能:

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.

細胞の位置付け:

Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.

サブユニット構造:

Interacts with EIF2AK4/GCN2; this interaction occurs under endoplasmic reticulum (ER) stress, hypothermic and amino acid starving stress conditions and inhibits EIF2AK4/GCN2 kinase activity. Interacts with EIF2AK3 (By similarity). Interacts with EIF2AK2. Forms a trimeric complex with DNAJB1 and HSPA8. Interacts with THAP12.

タンパク質ファミリー:

The J domain mediates interaction with HSPA8.

Binding to misfolded proteins is mediated by a hydrophobic patch forming a large groove within the first two TPR repeats.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Influenza A.

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