MMP12 Antibody - #DF7686
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| 製品: | MMP12 Antibody |
| カタログ: | DF7686 |
| タンパク質の説明: | Rabbit polyclonal antibody to MMP12 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Monkey |
| 予測: | Rabbit |
| 分子量: | 54 kDa; 54kD(Calculated). |
| ユニプロット: | P39900 |
| RRID: | AB_2841157 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7686, RRID:AB_2841157.
折りたたみ／展開
EC 3.4.24.65; HME; Macrophage elastase; Macrophage metalloelastase; Macrophage metaloelastase; Matrix metallopeptidase 12 (macrophage elastase); Matrix metalloprotease 12; Matrix metalloproteinase-12; ME; MGC138506; MME; MMP 12; MMP-12; Mmp12; MMP12_HUMAN;
免疫原
- P39900 MMP12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKFLLILLLQATASGALPLNSSTSLEKNNVLFGERYLEKFYGLEINKLPVTKMKYSGNLMKEKIQEMQHFLGLKVTGQLDTSTLEMMHAPRCGVPDVHHFREMPGGPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINTGMADILVVFARGAHGDFHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFFFKDRFFWLKVSERPKTSVNLISSLWPTLPSGIEAAYEIEARNQVFLFKDDKYWLISNLRPEPNYPKSIHSFGFPNFVKKIDAAVFNPRFYRTYFFVDNQYWRYDERRQMMDPGYPKLITKNFQGIGPKIDAVFYSKNKYYYFFQGSNQFEYDFLLQRITKTLKSNSWFGC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P39900 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y113 | Phosphorylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| Y414 | Phosphorylation | Uniprot | |
| Y434 | Phosphorylation | Uniprot |
研究背景
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Secreted>Extracellular space>Extracellular matrix.
Found in alveolar macrophages but not in peripheral blood monocytes.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Belongs to the peptidase M10A family.
参考文献
Application: WB Species: Mouse Sample: NR8383 cells
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