IPO9 Antibody - #DF7690
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7690, RRID:AB_2841161.
折りたたみ／展開
Imp9; Imp9a; Imp9b; Importin-9; Importin-9a; Ipo9; IPO9_HUMAN; Ran-binding protein 9; RanBP9;
免疫原
- Q96P70 IPO9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAAAAGAASGLPGPVAQGLKEALVDTLTGILSPVQEVRAAAEEQIKVLEVTEEFGVHLAELTVDPQGALAIRQLASVILKQYVETHWCAQSEKFRPPETTERAKIVIRELLPNGLRESISKVRSSVAYAVSAIAHWDWPEAWPQLFNLLMEMLVSGDLNAVHGAMRVLTEFTREVTDTQMPLVAPVILPEMYKIFTMAEVYGIRTRSRAVEIFTTCAHMICNMEELEKGAAKVLIFPVVQQFTEAFVQALQIPDGPTSDSGFKMEVLKAVTALVKNFPKHMVSSMQQILPIVWNTLTESAAFYVRTEVNYTEEVEDPVDSDGEVLGFENLVFSIFEFVHALLENSKFKSTVKKALPELIYYIILYMQITEEQIKVWTANPQQFVEDEDDDTFSYTVRIAAQDLLLAVATDFQNESAAALAAAATRHLQEAEQTKNSGTEHWWKIHEACMLALGSVKAIITDSVKNGRIHFDMHGFLTNVILADLNLSVSPFLLGRALWAASRFTVAMSPELIQQFLQATVSGLHETQPPSVRISAVRAIWGYCDQLKVSESTHVLQPFLPSILDGLIHLAAQFSSEVLNLVMETLCIVCTVDPEFTASMESKICPFTIAIFLKYSNDPVVASLAQDIFKELSQIEACQGPMQMRLIPTLVSIMQAPADKIPAGLCATAIDILTTVVRNTKPPLSQLLICQAFPAVAQCTLHTDDNATMQNGGECLRAYVSVTLEQVAQWHDEQGHNGLWYVMQVVSQLLDPRTSEFTAAFVGRLVSTLISKAGRELGENLDQILRAILSKMQQAETLSVMQSLIMVFAHLVHTQLEPLLEFLCSLPGPTGKPALEFVMAEWTSRQHLFYGQYEGKVSSVALCKLLQHGINADDKRLQDIRVKGEEIYSMDEGIRTRSKSAKNPERWTNIPLLVKILKLIINELSNVMEANAARQATPAEWSQDDSNDMWEDQEEEEEEEEDGLAGQLLSDILATSKYEEDYYEDDEEDDPDALKDPLYQIDLQAYLTDFLCQFAQQPCYIMFSGHLNDNERRVLQTIGI
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96P70 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| K277 | Acetylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| T435 | Phosphorylation | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| S489 | Phosphorylation | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K876 | Ubiquitination | Uniprot | |
| K884 | Ubiquitination | Uniprot | |
| Y889 | Phosphorylation | Uniprot | |
| S890 | Phosphorylation | Uniprot | |
| T897 | Phosphorylation | Uniprot | |
| T909 | Phosphorylation | Uniprot | |
| T938 | Phosphorylation | Uniprot | |
| S971 | Phosphorylation | Uniprot |
研究背景
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates the nuclear import of RPS7, RPL18A, RPL6, histone H2A, histone H2B and histone. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. Mediates the nuclear import of actin (By similarity).
Cytoplasm. Nucleus.
Binds with high affinity to RPS7 and RPL18A. The binding is coupled to RanGTP cycles. May bind H2A, H3, H4 histones, RPL4 and RPL6 with low affinity. Interacts with PPP2R1A and PPP2R1B.
Belongs to the importin beta family.
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