LDLR Antibody - #DF7696
(1)
(3)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7696, RRID:AB_2841167.
折りたたみ／展開
FH; FHC; LDL R; LDL receptor; LDLCQ2; Ldlr; LDLR_HUMAN; Low Density Lipoprotein Receptor; Low density lipoprotein receptor class A domain containing protein 3; Low density lipoprotein receptor familial hypercholesterolemia; Low-density lipoprotein receptor;
免疫原
- P01130 LDLR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P01130 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K38 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| N156 | N-Glycosylation | Uniprot | |
| N272 | N-Glycosylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| Y465 | Phosphorylation | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| K596 | Ubiquitination | Uniprot | |
| K603 | Ubiquitination | Uniprot | |
| N657 | N-Glycosylation | Uniprot | |
| T713 | Phosphorylation | Uniprot | |
| T721 | O-Glycosylation | Uniprot | |
| T724 | O-Glycosylation | Uniprot | |
| S725 | O-Glycosylation | Uniprot | |
| T726 | O-Glycosylation | Uniprot | |
| K730 | Ubiquitination | Uniprot | |
| S732 | O-Glycosylation | Uniprot | |
| S733 | O-Glycosylation | Uniprot | |
| T734 | O-Glycosylation | Uniprot | |
| T738 | O-Glycosylation | Uniprot | |
| T741 | O-Glycosylation | Uniprot | |
| T742 | O-Glycosylation | Uniprot | |
| T743 | O-Glycosylation | Uniprot | |
| S750 | O-Glycosylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| Y828 | Phosphorylation | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| Y845 | Phosphorylation | Uniprot | |
| S846 | Phosphorylation | Uniprot | |
| Y847 | Phosphorylation | Uniprot |
研究背景
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins.
(Microbial infection) Acts as a receptor for Vesicular stomatitis virus.
(Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
N- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation.
Cell membrane>Single-pass type I membrane protein. Membrane>Clathrin-coated pit. Golgi apparatus. Early endosome. Late endosome. Lysosome.
Note: Rapidly endocytosed upon ligand binding.
Interacts (via NPXY motif) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif (By similarity). Interacts (via NPXY motif) with LDLRAP1 (via PID domain). Interacts with ARRB1. Interacts with SNX17. Interacts with the full-length immature form of PCSK9 (via C-terminus).
(Microbial infection) Interacts with vesicular stomatitis virus glycoprotein.
(Microbial infection) May interact with HIV-1 Tat.
The NPXY motif mediates the interaction with the clathrin adapter DAB2 and with LDLRAP1 which are involved in receptor internalization. A few residues outside the motif also play a role in the interaction.
Belongs to the LDLR family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Endocrine system > Ovarian steroidogenesis.
· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.
· Organismal Systems > Digestive system > Cholesterol metabolism.
参考文献
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