IRS4 Antibody - #DF7698
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7698, RRID:AB_2841169.
折りたたみ／展開
160 kDa phosphotyrosine protein; Insulin receptor substrate 4; IRS 4; PY160;
免疫原
- O14654 IRS4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASCSFTRDQATRRLRGAAAAAAAALAAVVTTPLLSSGTPTALIGTGSSCPGAMWLSTATGSRSDSESEEEDLPVGEEVCKRGYLRKQKHGHRRYFVLKLETADAPARLEYYENARKFRHSVRAAAAAAAAAASGAAIPPLIPPRRVITLYQCFSVSQRADARYRHLIALFTQDEYFAMVAENESEQESWYLLLSRLILESKRRRCGTLGAQPDGEPAALAAAAAAEPPFYKDVWQVIVKPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVVVQLLSIRRCGHSEQYFFLEVGRSTVIGPGELWMQVDDCVVAQNMHELFLEKMRALCADEYRARCRSYSISIGAHLLTLLSARRHLGLVPLEPGGWLRRSRFEQFCHLRAIGDGEDEMLFTRRFVTPSEPVAHSRRGRLHLPRGRRSRRAVSVPASFFRRLAPSPARPRHPAEAPNNGARLSSEVSGSGSGNFGEEGNPQGKEDQEGSGGDYMPMNNWGSGNGRGSGGGQGSNGQGSSSHSSGGNQCSGEGQGSRGGQGSNGQGSGGNQCSRDGQGTAGGHGSGGGQRPGGGHGSGGGQGPGDGHGSGGGKNSGGGKGSGSGKGSDGDGERGKSLKKRSYFGKLTQSKQQQMPPPPPPPPPPPPAGGTGGKGKSGGRFRLYFCVDRGATKECKEAKEVKDAEIPEGAARGPHRARAFDEDEDDPYVPMRPGVATPLVSSSDYMPMAPQNVSASKKRHSRSPFEDSRGYMMMFPRVSPPPAPSPPKAPDTNKEDDSKDNDSESDYMFMAPGAGAIPKNPRNPQGGSSSKSWSSYFSLPNPFRSSPLGQNDNSEYVPMLPGKFLGRGLDKEVSYNWDPKDAASKPSGEGSFSKPGDGGSPSKPSDHEPPKNKAKRPNRLSFITKGYKIKPKPQKPTHEQREADSSSDYVNMDFTKRESNTPAPSTQGLPDSWGIIAEPRQSAFSNYVNVEFGVPFPNPANDLSDLLRAIPRANPLSLDSARWPLPPLPLSATGSNAIEEEGDYIEVIFNSAMTPAMALADSAIRYDAETGRIYVVDPFSECCMDISLSPSRCSEPPPVARLLQEEEQERRRPQSRSQSFFAAARAAVSAFPTDSLERDLSPSSAPAVASAAEPTLALSQVVAAASALAAAPGIGAAAAAAGFDSASARWFQPVANAADAEAVRGAQDVAGGSNPGAHNPSANLARGDNQAGGAAAAAAAPEPPPRSRRVPRPPEREDSDNDDDTHVRMDFARRDNQFDSPKRGR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14654 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| T7 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| R384 | Methylation | Uniprot | |
| R398 | Methylation | Uniprot | |
| T401 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| R410 | Methylation | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| R442 | Methylation | Uniprot | |
| R444 | Methylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| S517 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| T552 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| T620 | Phosphorylation | Uniprot | |
| S622 | Phosphorylation | Uniprot | |
| R661 | Methylation | Uniprot | |
| K671 | Acetylation | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| R684 | Methylation | Uniprot | |
| Y700 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | Uniprot | |
| Y717 | Phosphorylation | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| R741 | Methylation | Uniprot | |
| Y743 | Phosphorylation | Uniprot | |
| R749 | Methylation | Uniprot | |
| S751 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | Uniprot | |
| S777 | Phosphorylation | Uniprot | |
| Y779 | Phosphorylation | Uniprot | |
| K803 | Ubiquitination | Uniprot | |
| S804 | Phosphorylation | Uniprot | |
| Y808 | Phosphorylation | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| S817 | Phosphorylation | Uniprot | |
| S826 | Phosphorylation | Uniprot | |
| Y828 | Phosphorylation | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| R839 | Methylation | Uniprot | |
| K843 | Ubiquitination | Uniprot | |
| S856 | Phosphorylation | Uniprot | |
| S859 | Phosphorylation | Uniprot | |
| S863 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| S872 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| S877 | Phosphorylation | Uniprot | |
| K897 | Ubiquitination | Uniprot | |
| R913 | Methylation | Uniprot | |
| S919 | Phosphorylation | Uniprot | |
| Y921 | Phosphorylation | Uniprot | |
| K928 | Ubiquitination | Uniprot | |
| T938 | Phosphorylation | Uniprot | |
| R952 | Methylation | Uniprot | |
| Y1038 | Phosphorylation | Uniprot | |
| Y1046 | Phosphorylation | Uniprot | |
| S1061 | Phosphorylation | Uniprot | |
| S1091 | Phosphorylation | Uniprot | |
| R1097 | Methylation | Uniprot | |
| S1107 | Phosphorylation | Uniprot | |
| R1176 | Methylation | Uniprot | |
| S1231 | Phosphorylation | Uniprot | |
| T1237 | Phosphorylation | Uniprot | |
| S1252 | Phosphorylation | Uniprot | |
| K1254 | Ubiquitination | Uniprot |
研究背景
Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.
Phosphorylated on tyrosine residues in response to both insulin and IGF1 signaling. Phosphorylated on Tyr-921 in response to FGF2 signaling. Phosphorylation of Tyr-921 is required for GRB2, phospholipase C-gamma and phosphatidylinositol 3-kinase interaction.
Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Expressed in myoblasts. Expressed in liver and hepatocellular carcinoma.
Interacts with SOCS6 in response to stimulation with either insulin or IGF1 (By similarity). Interacts with CRK and CRKL. Interaction with CRK is stronger than with CRKL. Interacts with CRK via the phosphorylated YXXM motifs. Interacts with GRB2 and PIK3R1. Interacts with PLC-gamma, SHC1, PTK6, PPP4C and NISCH.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.
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