Dicer Antibody - #DF7706
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7706, RRID:AB_2841176.
折りたたみ／展開
DCR; DCR1; Dicer 1 ribonuclease type III; Dicer; DICER_HUMAN; DICER1; Double-strand-specific ribonuclease; Endoribonuclease Dicer; Helicase MOI; Helicase with RNase motif; HERNA; KIAA0928; MNG1; RMSE2;
免疫原
- Q9UPY3 DICER_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UPY3 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S82 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| Y452 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| S643 | Phosphorylation | Uniprot | |
| K652 | Ubiquitination | Uniprot | |
| Y664 | Phosphorylation | Uniprot | |
| Y668 | Phosphorylation | Uniprot | |
| Y819 | Phosphorylation | Uniprot | |
| T820 | Phosphorylation | Uniprot | |
| S828 | Phosphorylation | Uniprot | |
| S962 | Phosphorylation | Uniprot | |
| T967 | Phosphorylation | Uniprot | |
| T1001 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot | |
| K1023 | Ubiquitination | Uniprot | |
| Y1091 | Phosphorylation | Uniprot | |
| Y1121 | Phosphorylation | Uniprot | |
| C1122 | S-Nitrosylation | Uniprot | |
| S1142 | Phosphorylation | Uniprot | |
| S1160 | Phosphorylation | Uniprot | |
| Y1204 | Phosphorylation | Uniprot | |
| S1252 | Phosphorylation | Uniprot | |
| S1255 | Phosphorylation | Uniprot | |
| S1280 | Phosphorylation | Uniprot | |
| T1329 | Phosphorylation | Uniprot | |
| Y1330 | Phosphorylation | Uniprot | |
| K1350 | Ubiquitination | Uniprot | |
| K1391 | Acetylation | Uniprot | |
| K1433 | Ubiquitination | Uniprot | |
| Y1438 | Phosphorylation | Uniprot | |
| Y1445 | Phosphorylation | Uniprot | |
| S1460 | Phosphorylation | Uniprot | |
| K1465 | Ubiquitination | Uniprot | |
| K1466 | Ubiquitination | Uniprot | |
| S1468 | Phosphorylation | Uniprot | |
| S1470 | Phosphorylation | Uniprot | |
| Y1479 | Phosphorylation | Uniprot | |
| K1595 | Ubiquitination | Uniprot | |
| S1612 | Phosphorylation | Uniprot | |
| K1615 | Ubiquitination | Uniprot | |
| S1728 | Phosphorylation | Uniprot | |
| S1852 | Phosphorylation | Uniprot | |
| S1868 | Phosphorylation | Uniprot | |
| S1922 | Phosphorylation | Uniprot |
研究背景
Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.
Cytoplasm.
Component of the RISC loading complex (RLC), or micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2; DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. Note that the trimeric RLC/miRLC is also referred to as RISC. Interacts with DHX9, AGO1, PIWIL1 and PRKRA. Associates with the 60S ribosome. Interacts with BCDIN3D. Interacts with AGO2, TARBP2, EIF6, MOV10 and RPL7A (60S ribosome subunit); they form a large RNA-induced silencing complex (RISC).
Belongs to the helicase family. Dicer subfamily.
研究領域
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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