IRE1 Antibody | Affinity Biosciences

製品:	IRE1 Antibody
カタログ:	DF7709
タンパク質の説明:	Rabbit polyclonal antibody to IRE1
アプリケーション:	WB IHC IF/ICC
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量:	110 kDa; 110kD(Calculated).
ユニプロット:	O75460
RRID:	AB_2841178

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:1000-3000, IF/ICC 1:200-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(86%)

クローナリティ:

Polyclonal

特異性:

IRE1 Antibody detects endogenous levels of total IRE1.

RRID:

AB_2841178
引用形式: Affinity Biosciences Cat# DF7709, RRID:AB_2841178.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

Endoplasmic reticulum (ER) to nucleus signalling 1; Endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Endoribonuclease; ER to nucleus signaling 1; ERN 1; Ern1; ERN1_HUMAN; hIRE 1p; hIRE1p; Inositol requiring 1; Inositol requiring 1, S. cerevisiae, homolog of; Inositol requiring enzyme 1, S. cerevisiae, homolog of; Inositol requiring protein 1; inositol-requiring enzyme 1; Inositol-requiring protein 1; IRE 1; IRE 1a; IRE 1P; Ire1 alpha; Ire1-alpha; IRE1a; Ire1alpha; IRE1P; MGC163277; MGC163279; Protein kinase/endoribonuclease; RGD1559716; Serine/threonine protein kinase/endoribonuclease IRE1;

免疫原

免疫原:

Uniprot:

O75460(ERN1_HUMAN) >>Visit HPA database.

遺伝子(ID):

ERN1(2081)

発現特異性:

O75460 ERN1_HUMAN:

Ubiquitously expressed. High levels observed in pancreatic tissue.

タンパク質配列:

O75460 ERN1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75460 基板として

O75460

Site	PTM Type	Enzyme	Source
T49	Phosphorylation		Uniprot
K88	Ubiquitination		Uniprot
K95	Ubiquitination		Uniprot
T132	Phosphorylation		Uniprot
Y161	Phosphorylation		Uniprot
Y166	Phosphorylation		Uniprot
Y179	Phosphorylation		Uniprot
T283	Phosphorylation		Uniprot
Y289	Phosphorylation		Uniprot
K374	Acetylation		Uniprot
K374	Ubiquitination		Uniprot
K485	Ubiquitination		Uniprot
S533	Phosphorylation		Uniprot
S536	Phosphorylation		Uniprot
S544	Phosphorylation		Uniprot
S548	Phosphorylation		Uniprot
S551	Phosphorylation		Uniprot
Y628	Phosphorylation	O75460 (ERN1)	Uniprot
K633	Ubiquitination		Uniprot
K706	Ubiquitination		Uniprot
S724	Phosphorylation	O75460 (ERN1)	Uniprot
S726	Phosphorylation		Uniprot
S729	Phosphorylation		Uniprot
K851	Ubiquitination		Uniprot
K860	Ubiquitination		Uniprot
T973	Phosphorylation		Uniprot

PTMs - O75460 酵素として

O75460

Substrate	Site	Source
O75460 (ERN1)	Y628	Uniprot
O75460 (ERN1)	S724	Uniprot

研究背景

O75460_ERN1_HUMAN

機能:

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A.

PTMs:

Autophosphorylated following homodimerization. Autophosphorylation promotes activation of the endoribonuclease domain.

ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities.

細胞の位置付け:

Endoplasmic reticulum membrane>Single-pass type I membrane protein.

組織特異性:

Ubiquitously expressed. High levels observed in pancreatic tissue.

サブユニット構造:

Monomer. Homodimer; disulfide-linked; homodimerization takes place in response to endoplasmic reticulum stress and promotes activation of the kinase and endoribonuclease activities. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Interacts (via the luminal region) with DNAJB9/ERdj4; interaction takes place in unstressed cells and promotes recruitment of HSPA5/BiP. Interacts (via the luminal region) with HSPA5/BiP; HSPA5/BiP is a negative regulator of the unfolded protein response (UPR) that prevents homodimerization of ERN1/IRE1 and subsequent activation of the protein. Interacts with PDIA6, a negative regulator of the UPR; the interaction is direct and disrupts homodimerization. Interacts with DAB2IP (via PH domain); the interaction occurs in a endoplasmic reticulum stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1/IRE1 (By similarity). Interacts with TAOK3 and TRAF2. Interacts with RNF13.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

参考文献

1). Upregulation of BCL-2 by acridone derivative through gene promoter i-motif for alleviating liver damage of NAFLD/NASH. NUCLEIC ACIDS RESEARCH, 2020 (PubMed: 32710621) [IF=14.9]

Application: WB Species: mouse Sample: liver

Figure 7. Effect of A22 on ameliorating apoptosis, ER stress, inflammation, metabolic syndrome, and fibrogenesis in HF diet-fed mice. (A) Effect of A22 on BCL-2 gene transcription. (B) Effect of A22 on BAX gene transcription. (C) Effect of A22 on expressions of apoptosis-related proteins in liver. The extracted proteins from the liver were immunoblotted with specific antibodies, and quantified based on the loading control of ACTIN. (D) Effect of A22 on ER stress. The UPR proteins (IRE-1, PERK, elF-2 and CHOP) were analyzed by using western Blot. (E) Effect of A22 on expressions of inflammatory factors. (F) Effect of A22 on expressions of fibrogenic proteins.

2). Menaquinone-4 prevents medication-related osteonecrosis of the jaw through the SIRT1 signaling-mediated inhibition of cellular metabolic stresses-induced osteoblast apoptosis. Free Radical Biology and Medicine, 2023 (PubMed: 37364692) [IF=7.4]

3). Aqueous Extract of Pepino Leaves Ameliorates Palmitic Acid-Induced Hepatocellular Lipotoxicity via Inhibition of Endoplasmic Reticulum Stress and Apoptosis. Antioxidants, 2021 (PubMed: 34204987) [IF=7.0]

Application: WB Species: Human Sample: HepG2 Cells

Figure 5 AEPL attenuated ER stress while PA exposure. (a) ER stress-related protein levels were analyzed by Western blotting. (b–d) GRP78, phosphorylated PERK, and phosphorylated IRE1αwere normalized to PERK, IRE1α, and β-actin of each sample. (e) SREBP-1 level in each group was normalized to β-actin. All data were presented as mean ± SD of at least three independent experiments. * p < 0.05 vs. control cells; # p < 0.05 vs. PA-treated cells.

4). Sesamol supplementation alleviates nonalcoholic steatohepatitis and atherosclerosis in high-fat, high carbohydrate and high-cholesterol diet-fed rats. Food & Function, 2021 (PubMed: 34606548) [IF=6.1]

Application: WB Species: Rat Sample:

Fig. 5 Sesamol regulated the hepatic ERS-IRE1 signaling pathway and NLRP3 expression in HF-HCC diet-fed rats. (A) Representative images of the western blot analyses from hepatic Bip, IRE1, phospho-IRE1, and NLRP3 expression. (B) The semi-quantitative analysis of Bip, and (C and D) representative images of the IHC analyses from hepatic phospho-IRE1 and NLRP3 expression (samples from 3 rats in each group were analyzed). Values are expressed as means ± SEM (n = 3); ###P < 0.001, versus control; ***P < 0.001, versus HF-HCC.

5). Pterostilbene Prevents Tunicamycin-Induced Intestinal Barrier Damage by Targeting Endoplasmic Reticulum Stress, Oxidative Stress, Autophagy, and Gut Microbiota. Journal of Agricultural and Food Chemistry, 2022 (PubMed: 36225099) [IF=6.1]

6). Icariin improves the cognitive function of APP/PS1 mice via suppressing endoplasmic reticulum stress. LIFE SCIENCES, 2019 (PubMed: 31400352) [IF=6.1]

Application: WB Species: mouse Sample: hippocampus

Fig.5| Effects of ICA on ER stress in the hippocampus of APP/PS1 mice. ER stress was activated in the hippocampus of APP/PS1 mice evidenced by the high levels of GRP78, ATF6, and p-IRE1α. ICA treatment decreased GRP78 level, not affected the ATF6, IRE1α and IRE1αphosphorylation. (A) Immunoblots.

7). Resveratrol protects against CIH-induced myocardial injury by targeting Nrf2 and blocking NLRP3 inflammasome activation. LIFE SCIENCES, 2020 (PubMed: 31996295) [IF=6.1]

Application: WB Species: Rat Sample: heart tissue

Fig. 4. Resveratrol treatment weakened cardiac ER stress in rats exposed to CIH. A. Representative western blot images related to ER stress. B. p-PERK/ERK ratio. C. p-IRE/IRE ratio. D. GRP78 expression. n = 6. *p < 0.05 vs. control group; #p < 0.05 vs. CIH group. △ There was a CIH × resveratrol interaction.

8). Flavonoids from Hippophae rhamnoides Linn. Revert Doxorubicin-Induced Cardiotoxicity through Inhibition of Mitochondrial Dysfunction in H9c2 Cardiomyoblasts In Vitro. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 36834585) [IF=5.6]

9). Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Frontiers in Pharmacology, 2019 (PubMed: 31632274) [IF=5.6]

Application: WB Species: rat Sample: liver

FIGURE 4 | PA treatment attenuated HFD-induced ER stress in rats. (A) Representative immunoreactive bands of GRP78, PERK, p-PERK, IRE1α, p-IRE1α, and ATF6

10). Involvement of miR‐27a‐3p in diabetic nephropathy via affecting renal fibrosis, mitochondrial dysfunction, and endoplasmic reticulum stress. JOURNAL OF CELLULAR PHYSIOLOGY, 2021 (PubMed: 32691413) [IF=5.6]

Application: WB Species: mice Sample: kidney

FIGURE 4 Effect of miR‐27a‐3p on endoplasmic reticulum stress and apoptosis in the renal cortex of db/db mice. (a) Western blot analysis of p‐IRE1α, IRE1α, XBP1s, p‐PERK, PERK, p‐eIF2α, eIF2α, and CHOP proteins in the kidney tissues of mice. (b) Apoptosis in renal cortex was visualized by TUNEL immunohistochemical staining. Data are presented as mean ± SD (n = 6). CHOP, C/EBP homologous protein; eIF2α, eukaryotic initiation factor 2 α; IRE1α, inositol‐requiring transmembrane kinase/endoribonuclease 1α; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; XBP1, X‐box binding protein 1

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IRE1 Antibody - #DF7709

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