NEDD4L Antibody - #DF7724
| 製品: | NEDD4L Antibody |
| カタログ: | DF7724 |
| タンパク質の説明: | Rabbit polyclonal antibody to NEDD4L |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 100~120kD; 112kD(Calculated). |
| ユニプロット: | Q96PU5 |
| RRID: | AB_2841192 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF7724, RRID:AB_2841192.
折りたたみ／展開
E3 ubiquitin protein ligase NEDD4 like protein; E3 ubiquitin-protein ligase NEDD4-like; KIAA0439; NED4L_HUMAN; NEDD4 2; NEDD4 2c; Nedd4-2; NEDD4-2a; NEDD4.2; NEDD4b; NEDD4L; NEDD4La; NEDD4Lb; NEDD4Lc; NEDD4Ld; NEDD4Le; NEDD4Lf; NEDD4Lg; NEDD4Lh; NEDL3; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase; Neural precursor cell expressed, developmentally down regulated 4 like; RSP5; Ubiquitin protein ligase Rsp5;
免疫原
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003).
- Q96PU5 NED4L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96PU5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | P17612 (PRKACA) , O00141 (SGK1) | Uniprot |
| T344 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| T367 | Phosphorylation | P17612 (PRKACA) , O00141 (SGK1) | Uniprot |
| T369 | Phosphorylation | Uniprot | |
| T375 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| Y380 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | P17612 (PRKACA) , O00141 (SGK1) , Q96BR1 (SGK3) | Uniprot |
| S449 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| Y485 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| K639 | Ubiquitination | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| Y660 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| T903 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Plays a role in dendrite formation by melanocytes. Involved in the regulation of TOR signaling. Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF.
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels.
Cytoplasm. Golgi apparatus. Endosome>Multivesicular body.
Note: May be recruited to exosomes by NDFIP1.
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Expressed in melanocytes.
Interacts with UBE2E3 (By similarity). Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase. Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A, SCN10A and CLCN5. Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with TNK2. Interacts with WNK1. Interacts with SGK1. Interacts (via C2 domain) with NPC2. Interacts with ARRDC4. Interacts with KCNQ1; promotes internalization of KCNQ1. Interacts (via domains WW1, 3 and 4) with USP36; the interaction inhibits ubiquitination of, at least, NTRK1, KCNQ2 and KCNQ3 by NEDD4L.
(Microbial infection) Interacts with Epstein-Barr virus LMP2A.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
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