製品: Choline Acetyltransferase Antibody
カタログ: DF7744
タンパク質の説明: Rabbit polyclonal antibody to Choline Acetyltransferase
アプリケーション: WB
反応性: Human
分子量: 83 kDa.; 83kD(Calculated).
ユニプロット: P28329
RRID: AB_2841211

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
Choline Acetyltransferase Antibody detects endogenous levels of total Choline Acetyltransferase.
RRID:
AB_2841211
引用形式: Affinity Biosciences Cat# DF7744, RRID:AB_2841211.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; Choline O acetyltransferase; Choline O-acetyltransferase; CLAT_HUMAN; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584;

免疫原

免疫原:

A synthesized peptide derived from human Choline Acetyltransferase, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MGLRTAKKRGLGGGGKWKREEGGGTRGRREVRPACFLQSGGRGDPGDVGGPAGNPGCSPHPRAATRPPPLPAHTPAHTPEWCGAASAEAAEPRRAGPHLCIPAPGLTKTPILEKVPRKMAAKTPSSEESGLPKLPVPPLQQTLATYLQCMRHLVSEEQFRKSQAIVQQFGAPGGLGETLQQKLLERQEKTANWVSEYWLNDMYLNNRLALPVNSSPAVIFARQHFPGTDDQLRFAASLISGVLSYKALLDSHSIPTDCAKGQLSGQPLCMKQYYGLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTNRDSLDMIERCICLVCLDAPGGVELSDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHVTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

PTMs - P28329 基板として

Site PTM Type Enzyme
K7 Acetylation
K16 Acetylation
K18 Acetylation
T25 Phosphorylation
T123 Phosphorylation
S125 Phosphorylation
S237 Phosphorylation
S244 Phosphorylation
T373 Phosphorylation P17252 (PRKCA) , P05771 (PRKCB) , P05129 (PRKCG)
S377 Phosphorylation
T462 Phosphorylation
S464 Phosphorylation Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB)
S465 Phosphorylation P17252 (PRKCA) , P05129 (PRKCG) , P05771 (PRKCB) , Q02156 (PRKCE)
S473 Phosphorylation
S475 Phosphorylation
T553 Phosphorylation
Y554 Phosphorylation
S558 Phosphorylation Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB) , Q02156 (PRKCE) , P17252 (PRKCA) , P05129 (PRKCG)
T574 Phosphorylation Q9UQM7 (CAMK2A) , Q13555 (CAMK2G)
S594 Phosphorylation Q02156 (PRKCE) , P05129 (PRKCG) , Q05513 (PRKCZ) , P17252 (PRKCA) , Q05655 (PRKCD) , P05771 (PRKCB)

研究背景

機能:

Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.

タンパク質ファミリー:

Belongs to the carnitine/choline acetyltransferase family.

研究領域

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Organismal Systems > Nervous system > Cholinergic synapse.

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